Natural gastric infection with Helicobacter pylori in monkeys: A model for spiral bacteria infection in humans

Dubois, A.; Fiala, Nancy; Heman-Ackah, Lillie M.; Drazek, E. S.; Tarnawski, Andrzej S.; Fishbein, William N.; Pérez-Pérez, Guillermo I.; Blaser, Martin J.

doi:10.1016/0016-5085(94)90392-1

Cited by 149 publications

(149 citation statements)

References 46 publications

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“…Antigenic cross-reactivity between H. pylori and H. heilmannii could limit application of conventional serology in monkeys, but was not observed in one study. 15 In our experience, immunocytochemical staining with anti-H. pylori antibodies did not discriminate between H. pylori and H. heilmannii because both organisms stained positive. Similar cross-reactivity was observed with human biopsy material by other investigators.…”

Section: Discussionmentioning

confidence: 59%

“…20 50 Gastric disease has been attributed to both organisms, but incidence and intensity of gastric disease tends to be greatest in primates infected with H. pylori. 7,15,18,25,34,35 An additional Helicobacter species, H. nemestrinae, has been isolated from the stomach of a pigtailed macaque and was considered to be nonpathogenic. 3 Several reports have documented the natural occurrence of Helicobacter-related infections, particularly H. pylori, in nonhuman primates.…”

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confidence: 99%

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An Epizootic of Lymphoplasmacytic Gastritis Attributed to Helicobacter pylori Infection in Cynomolgus Monkeys (Macaca fascicularis)

Reindel¹,

Fitzgerald²,

Breider³

et al. 1999

Vet Pathol

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Abstract. An epizootic of subclinical lymphoplasmacytic gastritis occurred in cynomolgus monkeys maintained at our research facility. Gastric pathology data and histologic sections of 63 adolescent monkeys (2.5-3.5 years old) sacrificed during the epizootic were reviewed. Localized to multifocal reddening of the gastric mucosa was noted grossly in 7 of 44 (16%) monkeys harboring Helicobacter pylori, but not in any of 19 monkeys in which these bacteria were not seen. Gastritis, characterized by accentuation of lymphoplasmacytic infiltrates in antral and to a lesser degree cardiac mucosa, occurred in 42 of 63 (67%) monkeys evaluated and in 42 of 44 (93%) monkeys in which H. pylori was observed microscopically. Two monkeys with H. pylori infection had infiltrate scores that overlapped with the upper limit of scores of H. pylori-negative animals. Coincident with accentuated infiltrates were gastric gland epithelial hyperplasia, reduction in mucin content of surface and gland epithelia, and comparatively minor infiltrates of neutrophils in superficial lamina propria and gastric glands. Antral mucosa thickness often exceeded 1.5 to 2 times normal. Antral mucosal erosions occurred in 7 of 44 (16%) monkeys with H. pylori. Argyrophilic bacteria morphologically consistent with H. pylori were present in antral and less commonly cardiac mucosal glands. Intensity of bacterial colonization correlated with lymphoplasmacytic infiltrates (r ϭ 0.754) and hyperplasia (r ϭ 0.700), although responses were quite variable. These bacteria were not detected in fundic mucosa except in instances where parietal cells were substantially depleted in glands coincident with localized increases in lamina propria inflammatory cell infiltrates. Helicobacter heilmannii-like organisms (HHLOs) were present in fundic glands of all 63 monkeys; colonization was often pronounced. Scores for fundic mucosal inflammation did not correlate with presence or intensity of colonization with HHLOs (r ϭ 0.005). Rather, fundic inflammation scores positively correlated with the antral inflammation scores (r ϭ 0.548). Bacteria morphologically, biochemically, and genetically consistent with H. pylori were cultured from gastric mucosal specimens confirming bacterial identification. These findings demonstrate that adolescent cynomolgus monkeys are susceptible to natural infection with H. pylori and develop many morphologic hallmarks of H. pylori-related gastritis in humans.Key words: Animal model; bacteria; gastritis; Gastrospirillum hominis; Helicobacter; Helicobacter heilmannii; Helicobacter pylori; monkey; nonhuman primate; spirochetes; stomach.Spirochetes or spiral-shaped bacteria have long been recognized as constituents of gastric microflora in various mammalian species. 8,29,36,48,55,61,62 38 Most Helicobacter species do not have strict host specificity. Rather, certain species have been isolated from multiple host species and experimental transmission among animal species has been shown. 19,22,31,37,41,52 Suspected zoonotic transmission has also been reported. ...

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Section: Discussionmentioning

confidence: 59%

mentioning

confidence: 99%

An Epizootic of Lymphoplasmacytic Gastritis Attributed to Helicobacter pylori Infection in Cynomolgus Monkeys (Macaca fascicularis)

Reindel¹,

Fitzgerald²,

Breider³

et al. 1999

Vet Pathol

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“…Children are often infected by a strain which a genetic fingerprint identical to that of their parents, and they maintain this genotype even after moving to a different environment [10]. Animals harbor organisms that resemble H. pylori, but with the exception of nonhuman primates [11] and, under particular circumstances, perhaps cats [12] and houseflies (Musca domestica) [13], none harbor H. pylori. In the same way, food-borne transmission has not been substantiated [14].…”

Section: General Characteristicsmentioning

confidence: 99%

Molecular Epidemiology of Helicobacter pylori in Brazilian Patients with Early Gastric Cancer and a Review to Understand the Prognosis of the Disease

Röesler¹,

Zeitune²

2014

Trends in Helicobacter Pylori Infection

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“…In the last few years animal models have been developed to study the pathogenesis of H. pylori infection, in gnotobiotic piglets, mice, mongolian gerbils and primates (4)(5)(6)(7)(8)(9).…”

Section: Introductionmentioning

confidence: 99%

RAPD-PCR, Histopathological and Serological Analysis of Four Mouse Strains Infected with Multiple Strains ofHelicobacter pylori

Wang

1999

Microbial Ecology in Health and Disease

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The aims of this study were to investigate: i) if a specific strain(s) became predominant in the stomach of mice infected with multiple strains of freshly isolated H. pylori; ii) if a time dependent selection of strains occurs; iii) to compare the degree of gastric inflammation in C57BL/6, CBA/Ca, BALB/cA and NMRI mice; and iv) to follow the serological response to the infection. Six weeks old mice were inoculated orally 3 times at 2-day intervals with 9 freshly isolated H. pylori strains. Five animals of each mouse strain were sacrificed at 2, 4, 10 and 16 weeks post-inoculation. Stomach smears were cultured on GAB-Camp agar at 37°C for 5 -10 days under microaerophilic conditions. Seven to 10 single H. pylori colonies from each animal were subcultured and identified by random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) analysis. Histopathology of the gastric mucosa and serum antibody response were also analysed for each animal.Most mice were found to be infected by 1 strain (119/95). In only a few animals were multiple strains recovered. Strain 119/95 constituted 90.5% of the 577 mouse-passaged isolates and when tested it expressed both vacuolating cytotoxin antigen (VacA) and cytotoxin associated antigen (CagA). The inflammation scores of murine stomachs and duodenum samples of infected animals were significantly higher than in non-infected control animals for all 4 mouse strains. No significant differences in inflammation score were observed for the mouse strains 16 weeks post-infection. The immune response was also higher in infected versus non-infected animals for all mouse strains. The specific serum anti H. pylori immune response was highest for the NMRI mice followed by BALB/cA, C57BL/6, and CBA/Ca mice.

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Natural gastric infection with Helicobacter pylori in monkeys: A model for spiral bacteria infection in humans

Cited by 149 publications

References 46 publications

An Epizootic of Lymphoplasmacytic Gastritis Attributed to Helicobacter pylori Infection in Cynomolgus Monkeys (Macaca fascicularis)

An Epizootic of Lymphoplasmacytic Gastritis Attributed to Helicobacter pylori Infection in Cynomolgus Monkeys (Macaca fascicularis)

Molecular Epidemiology of Helicobacter pylori in Brazilian Patients with Early Gastric Cancer and a Review to Understand the Prognosis of the Disease

RAPD-PCR, Histopathological and Serological Analysis of Four Mouse Strains Infected with Multiple Strains ofHelicobacter pylori

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