Natural genetic variation in the expression regulation of the chloroplast antioxidant system among <i>Arabidopsis thaliana</i> accessions

Juszczak, Ilona; Rudnik, Radoslaw; Pietzenuk, Björn; Baier, Margarete

doi:10.1111/j.1399-3054.2012.01602.x

Cited by 21 publications

(24 citation statements)

References 63 publications

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“…1). All tested accessions, except C24 (1.19 ± 0.19 in comparison with Col-0), accumulated at 20°C less Csd2 transcripts than Col-0, consistent with previous reports with different plant sets [13]. The strongest differences to Col-0 were detected for Cvi-0 (0.48 ± 0.07) and Ms-0 (0.56 ± 0.1), whose steady state Csd2 transcript levels were only half of that observed in Col-0 ( Fig.…”

Section: The Transcript Levels Encoding the Main Chloroplast Copper Psupporting

confidence: 92%

The strength of the miR398‐Csd2‐CCS1 regulon is subject to natural variation in Arabidopsis thaliana

Juszczak

Baier

2012

FEBS Letters

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Edited by Tamas DalmayKeywords: Chloroplast Cold Cu/Zn-superoxide dismutase miR398 Photooxidative stress a b s t r a c t miR398 links expression of the three major chloroplast copper proteins, plastocyanin, CCS1 and Csd2, to copper availability. miR398 abundance was stronger plastocyanin-controlled in accessions from cold and continental habitats (Kas-1, Ms-0, WS) than in Cvi-0 and Col-0. Target gene regulation was broken for Csd2 in Cvi-0 upon cold-treatment. Comparison of miR398 levels, target gene regulation as well as Ago1 and miR168 expression demonstrated that the miR398 regulon can be overwritten by accession specific transcriptional regulation in Cvi-0. It is concluded that the escape from the miRNA control of copper homeostasis is linked to adaptation of Cvi-0 to its harsh natural habitat.

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Section: The Transcript Levels Encoding the Main Chloroplast Copper Psupporting

confidence: 92%

The strength of the miR398‐Csd2‐CCS1 regulon is subject to natural variation in Arabidopsis thaliana

Juszczak

Baier

2012

FEBS Letters

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“…The plants were grown on soil as described previously (Heiber et al, 2007; Juszczak et al, 2012). Aseptic growth on plates was performed as described in Baier et al (2004).…”

Section: Methodsmentioning

confidence: 99%

“…2) or with QuantPrime-optimized primers (Arvidsson et al, 2008) spanning exon-intron borders (Suppl. 3) according to the MIQE standards, as described previously in Juszczak et al (2012), using actin cDNAs as references.…”

Section: Methodsmentioning

confidence: 99%

The radical induced cell death protein 1 (RCD1) supports transcriptional activation of genes for chloroplast antioxidant enzymes

et al. 2014

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The rimb1 (redox imbalanced 1) mutation was mapped to the RCD1 locus (radical-induced cell death 1; At1g32230) demonstrating that a major factor involved in redox-regulation genes for chloroplast antioxidant enzymes and protection against photooxidative stress, RIMB1, is identical to the regulator of disease response reactions and cell death, RCD1. Discovering this link let to our investigation of its regulatory mechanism. We show in yeast that RCD1 can physically interact with the transcription factor Rap2.4a which provides redox-sensitivity to nuclear expression of genes for chloroplast antioxidant enzymes. In the rimb1 (rcd1-6) mutant, a single nucleotide exchange results in a truncated RCD1 protein lacking the transcription factor binding site. Protein-protein interaction between full-length RCD1 and Rap2.4a is supported by H2O2, but not sensitive to the antioxidants dithiotreitol and ascorbate. In combination with transcript abundance analysis in Arabidopsis, it is concluded that RCD1 stabilizes the Rap2.4-dependent redox-regulation of the genes encoding chloroplast antioxidant enzymes in a widely redox-independent manner. Over the years, rcd1-mutant alleles have been described to develop symptoms like chlorosis, lesions along the leaf rims and in the mesophyll and (secondary) induction of extra- and intra-plastidic antioxidant defense mechanisms. All these rcd1 mutant characteristics were observed in rcd1-6 to succeed low activation of the chloroplast antioxidant system and glutathione biosynthesis. We conclude that RCD1 protects plant cells from running into reactive oxygen species (ROS)-triggered programs, such as cell death and activation of pathogen-responsive genes (PR genes) and extra-plastidic antioxidant enzymes, by supporting the induction of the chloroplast antioxidant system.

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“…Signal transduction takes place via CBF1-mediated induction of DELLA-protein expression and activation of gibberellin catabolism and arrests growth and development (Achard et al, 2008 ). Acclimation re-establishes photostasis and antagonizes ROS accumulation (Strand et al, 1999 ; Ensminger et al, 2006 ; Juszczak et al, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Natural Variation of Cold Deacclimation Correlates with Variation of Cold-Acclimation of the Plastid Antioxidant System in Arabidopsis thaliana Accessions

et al. 2016

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Temperature variations impact on the balance between photosynthetic electron transport and electron-consuming assimilation reactions and transiently increase generation of reactive oxygen species (ROS). Previous studies demonstrated that the expression of C-repeat binding factors (CBFs), which activate cold acclimation reactions, respond to chloroplast ROS signals and that cold deacclimation is partly halted for days after the transfer of acclimated plants to optimal growth conditions in four Arabidopsis accessions from cold-continental habitats. We hypothesized that these accessions differ from others in the regulation of the plastid antioxidant system (PAS). In the present study, we compared the expression intensity of the 12 most prominent PAS genes for peroxidases, superoxide dismutase and low molecular weight antioxidant regenerating enzymes in 10 Arabidopsis accessions with regulation of CBF and COR (cold regulated genes) transcript levels and cold-regulated metabolite levels prior to cold, after 2 week long cold acclimation and during the first 3 days of deacclimation. In the accessions with prolonged activation of cold responses, by trend, weaker induction of various cold-inducible PAS genes and stronger decreases in the expression of negatively cold-regulated PAS genes were observed. Low PAS gene expression delayed the post-cold decrease in H2O2 levels after transfer of the plants from cold to optimal growth conditions. We conclude that weaker expression of various PAS genes in the cold is an adapted strategy of the Arabidopsis accessions N14, N13, Ms-0, and Kas-1 to avoid full inactivation of cold-responses in the first days after the end of the cold period.

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Natural genetic variation in the expression regulation of the chloroplast antioxidant system among Arabidopsis thaliana accessions

Cited by 21 publications

References 63 publications

The strength of the miR398‐Csd2‐CCS1 regulon is subject to natural variation in Arabidopsis thaliana

The strength of the miR398‐Csd2‐CCS1 regulon is subject to natural variation in Arabidopsis thaliana

The radical induced cell death protein 1 (RCD1) supports transcriptional activation of genes for chloroplast antioxidant enzymes

Natural Variation of Cold Deacclimation Correlates with Variation of Cold-Acclimation of the Plastid Antioxidant System in Arabidopsis thaliana Accessions

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