Natural killer activity of kurloff cells: A direct demonstration on purified kurloff cell suspensions

Debout, Claire; Quillec, M.; Izard, Juan Francisco Martín

doi:10.1016/0008-8749(84)90034-0

Cited by 35 publications

(11 citation statements)

References 6 publications

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“…Previous reports have shown that Kurloff cells develop a cytotoxic natural killer activity (EREMIN et al, 1980;DEBOUT et al, 1984) and have a pan-T lymphocyte immunophenotype (DEBOUT et al, 1991). Their inclusion bodies exhibit both intense acid phosphatase (LANDEMORE et al, 1987) and alpha naphthyl acetate esterase activity (BURT et al, 1988).…”

Section: Discussionmentioning

confidence: 99%

Afferent Migration of the Kurloff Cells via Lymphatics into the Thymus of Estradiol-Treated Guinea Pigs.

Kato

Shimoda

1997

Archives of Histology and Cytology

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Section: Discussionmentioning

confidence: 99%

Afferent Migration of the Kurloff Cells via Lymphatics into the Thymus of Estradiol-Treated Guinea Pigs.

Kato

Shimoda

1997

Archives of Histology and Cytology

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“…Nevertheless, evidence is accumulating concerning their role in the immunological defense of the guinea pig (Cavia porcellus). At least in this species, KCs have natural killer activity (Debout et al 1984, Eremin et al 1980b. Moreover, increased numbers of KC appear associated with antileukemic effects in vivo (Debout et al 1995).…”

Section: Introductionmentioning

confidence: 98%

Dynamics of health of wild capybaras: biochemical and physiological parameters

et al. 2016

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Assessments of generic indices of health in wildlife populations increase our knowledge about the natural history of animal species and provide useful information for ecoepidemiological studies. However, there have been minimal studies on the parameters of hematological and biochemical parameters in wildlife. The goal of this study was to evaluate physiological dynamics of wild capybaras (Hydrochoerus hydrochaeris). To this aim, we evaluated biochemical and physiological parameters (blood cell counts, serum protein fractions, and spleen mass) and assessed their variability with sex, body condition, pregnancy status, and season. Samples (n = 67) were obtained from a managed population of free-ranging capybaras from Esteros del Iberá (Northeastern Argentina). The main findings reported were i) Kurloff cells (KC) were found in peripheral blood of both sexes, but levels were higher in females; ii) KC, eosinophil (E), and basophil (B) counts were positively associated with body condition; iii) pregnant females had different values of KC, B, and spleen mass than nonpregnant females; iv) albumin and KC (in females) and E and neutrophil (N) counts in males showed a seasonal pattern; and v) protein fractions of capybaras are reported for the first time. Life history traits such as pregnancy, seasonal processes, nutritional status, are reflected in some of the biochemical and physiological parameters evaluated here.

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“…After Triton X-100 extraction of KC AcPase from highly purified KC, a zymographic study has shown the sialidasesensitivity of these AcPases but their sialylation type was not determined [ 171. Using the same highly purified KC, the crude soluble cellular extracts obtained after dissociative extraction in 7 M urea were subjected to ion-exchange chromatography and the macromolecules responsible for the main histochemical affinities of the KB were separated and characterized: i) toluidine blue metachromatic and protease-resistant chondroitin-4-sulphate proteoglycans eluted at 0.5 M NaCl [16, 211; and ii) unbound on the anion exchange matrix, PAS-positive N-glycosylproteins (30-35 kDa) designated as KC major glycoproteins (mGP) [14, 201 among which a fraction containing NeuSa (2,6)Galp ( 1,4)GalcNAc oligosaccharide sequences was suggested to correspond to AcPase subunits [18]. However, after such an extraction in the presence of 7 M urea, no AcPase activity was detected.…”

Section: Introductionmentioning

confidence: 99%

Relationship between the acid phosphatases of the Kurloff body and the major 30–35 kDa glycoproteins of the Kurloff cell

Oulhaj

Taouji

Izard

et al. 1995

Biology of the Cell

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This study deals with the acid phosphatase (AcPase) of the Kurloff body (KB), a large (10 microns diameter) periodic acid-Schiff-positive lysosomal inclusion body present in Kurloff cells (KC). KC AcPase were extracted, with similar yields, either with non-ionic detergent solution or after Dounce homogenization in low ionic strength buffer suggesting that they mainly correspond to hydrosoluble AcPase. After DEAE-cellulose chromatography of such crude Dounce-extracts, 97% of KC AcPase activity was recovered in the unbound glycoprotein fraction (peak I)1). The main protein content consisted of, as testified by SDS-PAGE analysis, major KC glycoproteins of 30-35 kDa. Thus, KC AcPase, and particularly sialoAcPase, may be assumed to correspond to these N-glycosylproteins among which the presence of alpha (2,6) sialoglycoproteins was previously established. Following electrophoresis on a 4-15% gradient native polyacrylamide gel or isoelectric focusing, the two size populations (200 kDa and 500 kDa) and up to 20 isoforms of KC AcPase were respectively detected by zymography in peak I. After Clostridium-derived sialidase digestion of peak I, the highly active bands observed at pH 3.5-5.2 disappeared. These zymographic patterns were similar to those obtained with crude extracts. After Concanavalin A (ConA)-Sepharose chromatography of peak I, the single ConA-bound glucosamine-labelled peak, eluted at 200 methyl-alpha-D-mannopyranose, contained the AcPase activity while the ConA-unbound peak was devoid of any acid phosphatase activity. After SDS-PAGE analysis, the ConA-bound fraction appeared to correspond only to a single broad protein band in the 30-35 kDa zone.(ABSTRACT TRUNCATED AT 250 WORDS)

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Natural killer activity of kurloff cells: A direct demonstration on purified kurloff cell suspensions

Cited by 35 publications

References 6 publications

Afferent Migration of the Kurloff Cells via Lymphatics into the Thymus of Estradiol-Treated Guinea Pigs.

Afferent Migration of the Kurloff Cells via Lymphatics into the Thymus of Estradiol-Treated Guinea Pigs.

Dynamics of health of wild capybaras: biochemical and physiological parameters

Relationship between the acid phosphatases of the Kurloff body and the major 30–35 kDa glycoproteins of the Kurloff cell

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