Natural Killer and Dendritic Cell Contact in Lesional Atopic Dermatitis Skin –Malassezia-Influenced Cell Interaction

Buentke, Eva; Heffler, Lena C.; Wilson, Julia L.; Wallin, Robert P. A.; Löfman, Carl; Chambers, Benedict J.; Ljunggren, Hans‐Gustaf; Scheynius, Annika

doi:10.1046/j.1523-1747.2002.00132.x

Cited by 130 publications

(117 citation statements)

References 46 publications

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“…Nevertheless, this preliminary experiment provides novel evidence that NK cells are found in the skin of calves and therefore suggests that NK cells can migrate from the skin, through afferent lymphatic vessels and into the LNs. In humans, CD56 bright CD16 − NK cells are present in the dermis of healthy skin and were also found in skin from patients with atopic eczema/dermatitis, where they were in close contact with CD1a + dendritic cells 34, 35…”

Section: Discussionmentioning

confidence: 99%

Frequency and phenotype of natural killer cells and natural killer cell subsets in bovine lymphoid compartments and blood

et al. 2017

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SummaryNatural killer (NK) cells are widely distributed in lymphoid and non‐lymphoid tissues, but little is known about the recirculation of NK cells between blood and tissues. This is relevant to understanding recirculation in the steady‐state and also for determining the roles for NK cells in vaccine‐induced immunity and responses to infection. Therefore, the percentage of NK cells and their phenotype across peripheral blood, afferent lymph and lymph nodes in steady‐state conditions was investigated in cattle using the pseudo‐afferent lymphatic cannulation model. CD2+ CD25lo NK cells were the predominant subset of NK cells within the blood. In contrast, CD2− CD25hi NK cells were the main subset present within the skin‐draining afferent lymphatic vessels and lymph nodes, indicating that CD2− NK cells are the principal NK cell subset trafficking to lymph nodes via the afferent lymphatic vessel. Furthermore, a low percentage of NK cells were present in efferent lymph, which were predominantly of the CD2− subset, indicating that NK cells can egress from lymph nodes and return to circulation in steady‐state conditions. These compartmentalization data indicate that NK cells represent a population of recirculating lymphocytes in steady‐state conditions and therefore may be important during immune responses to vaccination or infection.

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Section: Discussionmentioning

confidence: 99%

Frequency and phenotype of natural killer cells and natural killer cell subsets in bovine lymphoid compartments and blood

et al. 2017

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“…Sites of NK-DC interactions may be either lymphoid organs (20 -24) or nonlymphoid peripheral tissues (25,26). In tissues, immature DCs (iDCs) have efficient mechanisms for the detection and uptake of pathogens.…”

Section: Nkp46 and Nkg2d Recognition Of Infected Dendritic Cells Is Nmentioning

confidence: 99%

NKp46 and NKG2D Recognition of Infected Dendritic Cells Is Necessary for NK Cell Activation in the Human Response to Influenza Infection

Draghi

Pashine

Sanjanwala

et al. 2007

The Journal of Immunology

181

170

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At an early phase of viral infection, contact and cooperation between dendritic cells (DCs) and NK cells activates innate immunity, and also influences recruitment, when needed, of adaptive immunity. Influenza, an adaptable fast-evolving virus, annually causes acute, widespread infections that challenge the innate and adaptive immunity of humanity. In this study, we dissect and define the molecular mechanisms by which influenza-infected, human DCs activate resting, autologous NK cells. Three events in NK cell activation showed different requirements for soluble mediators made by infected DCs and for signals arising from contact with infected DCs. IFN-α was mainly responsible for enhanced NK cytolysis and also important for CD69 up-regulation, whereas IL-12 was necessary for enhancing IFN-γ production. Increased CD69 expression and IFN-γ production, but not increased cytolysis, required recognition of influenza-infected DCs by two NK cell receptors: NKG2D and NKp46. Abs specific for these receptors or their known ligands (UL16-binding proteins 1–3 class I-like molecules for NKG2D and influenza hemagglutinin for NKp46) inhibited CD69 expression and IFN-γ production. Activation of NK cells by influenza-infected DCs and polyinosinic:polycytidylic acid (poly(I:C))-treated DCs was distinguished. Poly(I:C)-treated DCs did not express the UL16-binding protein 3 ligand for NKG2D, and in the absence of the influenza hemagglutinin there was no involvement of NKp46.

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“…In turn, NK cells cocultured with pDCs stimulated via TLR9 (with CpG-A) acquired cytolytic Freshly isolated NK cells were cocultured with autologous pDCs and CpG-A in the presence or in the absence of a mixture of neutralizing antibodies specific for IFN-␣ and for its receptor (indicated as aIFN). After 24 hours of coculture NK cells were assessed for their cytotoxic activity against allogeneic, freshly blood-derived pDCs (A) or against allogeneic iMDDCs (B) in a 4-hour 51 Cr-release assay. As a control, the same NK cells, activated or not with rhIL-2 for 24 hours, were tested for cytotoxicity against either pDCs or iMDDCs.…”

Section: Discussionmentioning

confidence: 99%

“…NK cells that had been cultured for 24 hours with autologous pDCs in the presence or in the absence of CpG-A, with the addition or not of neutralizing mAbs specific for IFN-␣ and for its receptor, were tested for cytolytic activity against allogeneic iMDDCs or allogeneic freshly bloodderived pDCs in a 4-hour 51 Cr-release assay as previously described. 8 In other experiments polyclonally activated NK cells were tested for their ability to lyse allogeneic iMDDCs or LPS-matured MDDCs or allogeneic pDCs, activated or not with rhIL-3 for 48 hours, in 4-hour 51 Cr-release assays.…”

Section: Cytolytic Assays and Flow Cytofluorimetric Analysismentioning

confidence: 99%

“…8 In other experiments polyclonally activated NK cells were tested for their ability to lyse allogeneic iMDDCs or LPS-matured MDDCs or allogeneic pDCs, activated or not with rhIL-3 for 48 hours, in 4-hour 51 Cr-release assays. For masking experiments mAbs of IgM isotype were added at the concentration of 10 g/mL.…”

Section: Cytolytic Assays and Flow Cytofluorimetric Analysismentioning

confidence: 99%

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Multidirectional interactions are bridging human NK cells with plasmacytoid and monocyte-derived dendritic cells during innate immune responses

Chiesa

Romagnani

Thiel

et al. 2006

Blood

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During innate immune responses, natural killer (NK) cells may interact with both plasmacytoid dendritic cells (pDCs) and monocyte-derived dendritic cells (MDDCs). We show that freshly isolated NK cells promote the release by pDCs of IFN-␣, in a CpG-dependent manner, whereas they induce IL-6 production in a CpG-independent manner. In turn pDCderived IFN-␣ up-regulates NK-mediated killing, whereas IL-6 could promote B-cell differentiation. We also show that expo- IntroductionInnate immune responses play a crucial role not only in controlling pathogen invasion, but also in instructing the adaptive immune system to initiate a specific response against infecting pathogens. 1 Natural killer (NK) cells and dendritic cells (DCs) represent crucial effector cells of the innate immune system. 2,3 Several reports have highlighted the importance of the reciprocal interaction between NK cells and DCs during the early stages of innate immune responses. [4][5][6][7][8][9] The NK/DC cross talk can occur within inflamed peripheral tissues and results in a potent DC-induced activation of NK-cell functions, which, in turn, exert a modulatory role on DC activity. Pathogen clearance mediated by innate effector mechanisms is, at least in part, the result of the DC-induced up-regulation of NK-cell cytotoxicity and of the release of antimicrobial cytokines from various cell types recruited at the inflammatory sites. 10,11 The reciprocal interactions between NK and DCs could regulate the quality of DCs undergoing maturation. For example, because NK cells are capable of killing immature DC (iDCs), the existence of an "editing" process has been suggested, by which DCs may or may not acquire the ability to prime Th1 immune responses. 12,13 Most reports regarding the cross talk between NK cells and DCs in humans have been focused on the interactions with monocyte-derived dendritic cells (MDDCs), whereas only limited information exists on the interaction with plasmacytoid DCs (pDCs). 14,15 pDCs are capable of producing large amounts of IFN-␣ in response to viral DNA or RNA. 16 This suggests that, together with NK cells, they play an important role in sensing viral infections and in promoting antiviral innate responses. We have previously shown that pDCs, on stimulation through TLR9, can activate NK cells to kill various types of tumor cells. Moreover, following TLR9 engagement, pDCs could induce a selective proliferation of the small NK-cell subset characterized by the CD56 bright phenotype. 15 In addition, IL-2-activated NK cells have been shown to promote pDC maturation and IFN-␣ release. 14 On the other hand, it is not clear whether, in humans, this event can actually occur during the early phases of innate immune responses in which T cells (ie, the only IL-2-producing cells) are not involved.In the present study we show that the regulatory effect of NK cells on pDC function does not strictly require exposure to IL-2, because also freshly isolated NK cells promoted pDC maturation and cytokine release. Moreover, an abundant IFN-␣ release b...

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Natural Killer and Dendritic Cell Contact in Lesional Atopic Dermatitis Skin –Malassezia-Influenced Cell Interaction

Cited by 130 publications

References 46 publications

Frequency and phenotype of natural killer cells and natural killer cell subsets in bovine lymphoid compartments and blood

Frequency and phenotype of natural killer cells and natural killer cell subsets in bovine lymphoid compartments and blood

NKp46 and NKG2D Recognition of Infected Dendritic Cells Is Necessary for NK Cell Activation in the Human Response to Influenza Infection

Multidirectional interactions are bridging human NK cells with plasmacytoid and monocyte-derived dendritic cells during innate immune responses

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