Natural killer cell activity in long term survivors of testicular cancer. Influence of cytostatic therapy and initial stage of disease

Krainer, Michael; Wolf, Herrmann; Michl, I.; Wiltschke, Christoph; Budinsky, Alexandra C.; Kaider, Alexandra; Kratzik, Christian; Eibl, Martha M.; Zielinski, Christoph

doi:10.1002/1097-0142(19950115)75:2<539::aid-cncr2820750217>3.0.co;2-4

Cited by 4 publications

(1 citation statement)

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“…Other reports have stated that tumours propagated as a result of NK activity depression, and that metastasis could be inhibited by elevating NK activity [41±43]. There have also been a number of cases of metastatic-free survival extension in tumour patients with high NK activity [44,45]. Since the effects of immune surveillance and the antitumour activities of NK cells can affect tumour growth, it is possible that the NK cells activated by DCs possess potent antitumour activity, and may be applicable to the clinical treatment of cancer patients.…”

Section: Discussionmentioning

confidence: 99%

Mechanism of NK cell activation induced by coculture with dendritic cells derived from peripheral blood monocytes

Amakata

Fujiyama

Andoh

et al. 2001

Clinical and Experimental Immunology

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SUMMARYDendritic cells (DCs) have been regarded as one of the effective antigen-presenting cells, but the relationship between DCs and lymphocytes, in particular natural killer (NK) cells, remains unclear. In this study, we evaluated how DCs interact with both lymphocytes and NK cells using a coculture system. The number of lymphocytes increased significantly when cocultured with DCs (1´8-fold increase). In particular, the proliferation of NK cells was prominent. Furthermore, the coculture of DCs with lymphocytes induced a marked increase in IL-12 and IFN-g secretion. When contact between the DCs and lymphocytes was prevented, the secretion of both IL-12 and IFN-g was markedly reduced. IFN-g production was completely blocked by an anti-IL-12 antibody, indicating that IFN-g secretion was dependent on IL-12 secretion. The stimulating effect of the DCs on the proliferation of the lymphocytes was partially suppressed by anti-IL-12 antibodies, and was completely attenuated when cellular contact was prevented. Furthermore, the NK cell proliferation induced by coculture with DCs was significantly blocked by the inhibition of the interaction of either CD40±CD40L or CD28±B7 molecule. The coculture with DCs enhanced NK activity by 40%, and this was partially suppressed by anti-IL-12 antibodies and was completely blocked by the inhibition of cell-to-cell contact. These results indicate that the activation of NK cells by DCs is partially mediated by IL-12 secretion, and that direct contact between DCs and NK cells play a major role in this response.

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Section: Discussionmentioning

confidence: 99%