Natural killer cells and dendritic epidermal γδ T cells orchestrate type 1 conventional DC spatiotemporal repositioning toward CD8+ T cells

Ghilas, Sonia; Ambrosini, Marc; Cancel, Jean Charles; Brousse, Carine; Massé, Marion; Lelouard, Hugues; Dalod, Marc; Crozat, Karine

doi:10.1016/j.isci.2021.103059

Cited by 25 publications

(32 citation statements)

References 98 publications

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“…Cluster 3 DETCs, displaying hallmarks of recent activation in unchallenged mice, could represent Skint1-licensed natural immunosurveillance responses to spontaneous epithelial perturbations from scratching/grooming or mutagenesis. Intriguingly, DETC-derived XCL1 reportedly recruits cross-presenting dendritic cells (DCs) to the epidermis 43 . Thus, XCL1 release from cluster 3 DETCs responding to episodic, low-level KC dysregulation might, in cases of infection or genotoxicity, facilitate neoantigen uptake by DCs, thereby initiating cytotoxic CD8 + T cell immunosurveillance.…”

Section: Discussionmentioning

confidence: 99%

Normality sensing licenses local T cells for innate-like tissue surveillance

et al. 2022

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The increasing implication of lymphocytes in general physiology and immune surveillance outside of infection poses the question of how their antigen receptors might be involved. Here, we show that macromolecular aggregates of intraepidermal γδ T cell antigen receptors (TCRs) in the mouse skin aligned with and depended on Skint1, a butyrophilin-like (BTNL) protein expressed by differentiated keratinocytes (KCs) at steady state. Interruption of TCR-mediated ‘normality sensing’ had no impact on γδ T cell numbers but altered their signature phenotype, while the epidermal barrier function was compromised. In addition to the regulation of steady-state physiology, normality sensing licensed intraepidermal T cells to respond rapidly to subsequent tissue perturbation by using innate tumor necrosis factor (TNF) superfamily receptors. Thus, interfering with Skint1-dependent interactions between local γδ T cells and KCs at steady state increased the susceptibility to ultraviolet B radiation (UVR)-induced DNA damage and inflammation, two cancer-disposing factors.

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Section: Discussionmentioning

confidence: 99%

Normality sensing licenses local T cells for innate-like tissue surveillance

et al. 2022

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“…However, there are other potential mechanisms of NK cell-mediated DC activation. GM-CSF produced by NK cells can activate cDC1 to mediate Th1 CD4 + T cell inflammatory responses in the lymph nodes ( 41 ). To determine whether NK cells also produce GM-CSF that may contribute to DC activation, we PO inoculated mice with T1L or PBS as a mock control and stained NK cells for intracellular GM-CSF expression.…”

Section: Resultsmentioning

confidence: 99%

NK cells contribute to reovirus-induced IFN responses and loss of tolerance to dietary antigen

Brigleb¹,

Kouame²,

Fiske³

et al. 2022

JCI Insight

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Celiac disease is an immune-mediated intestinal disorder that results from loss of oral tolerance (LOT) to dietary gluten. Reovirus elicits inflammatory Th1 cells and suppresses Treg responses to dietary antigen in a strain-dependent manner. Strain type 1 Lang (T1L) breaks oral tolerance, while strain type 3 Dearing reassortant virus (T3D-RV) does not. We discovered that intestinal infection by T1L in mice leads to the recruitment and activation of NK cells in mesenteric lymph nodes (MLNs) in a type I IFN–dependent manner. Once activated following infection, NK cells produce type II IFN and contribute to IFN-stimulated gene expression in the MLNs, which in turn induces inflammatory DC and T cell responses. Immune depletion of NK cells impairs T1L-induced LOT to newly introduced food antigen. These studies indicate that NK cells modulate the response to dietary antigen in the presence of a viral infection.

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“…MZ cDC1 functionally interact with CD169 hi marginal metallophillic macrophages (MMM) during bacterial infection [57][58][59] and with NK cells during viral infection. 60 CD169 hi MMM also facilitate cDC1 capture of circulating apoptotic cells in a process that contributes to maintenance of self-tolerance. 61,62 In a remarkable recent finding, during influenza infection a subset of cDC emigrated from lung mediastinal LN and trafficked to the spleen to prime splenic CD8 T cell responses.…”

Section: Gene Expression Data Indicate Minimal Expression Of S1pr1 Bymentioning

confidence: 99%

“…In adult mice, the role of cDC in the MZ maybe better defined for cDC1. MZ cDC1 functionally interact with CD169 hi marginal metallophillic macrophages (MMM) during bacterial infection 57‐59 and with NK cells during viral infection 60 . CD169 hi MMM also facilitate cDC1 capture of circulating apoptotic cells in a process that contributes to maintenance of self‐tolerance 61,62 …”

Section: Positioning the Sentinelsmentioning

confidence: 99%

Chemo‐ and mechanosensing by dendritic cells facilitate antigen surveillance in the spleen*

Liu

Duan

Cyster

2022

Immunological Reviews

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Spleen dendritic cells (DC) are critical for initiation of adaptive immune responses against blood‐borne invaders. Key to DC function is their positioning at sites of pathogen entry, and their abilities to selectively capture foreign antigens and promptly engage T cells. Focusing on conventional DC2 (cDC2), we discuss the contribution of chemoattractant receptors (EBI2 or GPR183, S1PR1, and CCR7) and integrins to cDC2 positioning and function. We give particular attention to a newly identified role in cDC2 for adhesion G‐protein coupled receptor E5 (Adgre5 or CD97) and its ligand CD55, detailing how this mechanosensing system contributes to splenic cDC2 positioning and homeostasis. Additional roles of CD97 in the immune system are reviewed. The ability of cDC2 to be activated by circulating missing self‐CD47 cells and to integrate multiple red blood cell (RBC)‐derived inputs is discussed. Finally, we describe the process of activated cDC2 migration to engage and prime helper T cells. Throughout the review, we consider the insights into cDC function in the spleen that have emerged from imaging studies.

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Natural killer cells and dendritic epidermal γδ T cells orchestrate type 1 conventional DC spatiotemporal repositioning toward CD8+ T cells

Cited by 25 publications

References 98 publications

Normality sensing licenses local T cells for innate-like tissue surveillance

Normality sensing licenses local T cells for innate-like tissue surveillance

NK cells contribute to reovirus-induced IFN responses and loss of tolerance to dietary antigen

Chemo‐ and mechanosensing by dendritic cells facilitate antigen surveillance in the spleen*

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