Natural lupeol level variation among castor accessions and the upregulation of lupeol synthesis in response to light

Li, Donghai; Zaman, Wajid; Lu, Jianjun; Niu, Qingqing; Zhang, Xuanhao; Ayaz, Asma; Saqib, Saddam; Yang, Baiming; Zhang, Jixing; Zhao, Huayan; Lü, Shiyou

doi:10.1016/j.indcrop.2022.116090

Cited by 17 publications

(9 citation statements)

References 33 publications

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“…The fragments per kilobase of exon model per million mapped reads (FPKM) technique was used to standardise the data. Utilising the ABI 7500 rapid Real-Time PCR instrument, qRT-PCR was carried out (Applied Biosystems, USA) [ 35 ]. The primers used for qRT-PCR are shown in Table S1 Primer pairs of four PC genes were designed using Oligo 7 [ 36 ], and the 2 −ΔΔCt method was used to calculate the gene expressions [ 37 , 38 ].…”

Section: Methodsmentioning

confidence: 99%

Identification and Characterization of Phytocyanin Family Genes in Cotton Genomes

Tufail

Yasir

Zuo

et al. 2023

Genes

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Phytocyanins (PCs) are a class of plant-specific blue copper proteins that have been demonstrated to play a role in electron transport and plant development. Through analysis of the copper ligand residues, spectroscopic properties, and domain architecture of the protein, PCs have been grouped into four subfamilies: uclacyanins (UCs), stellacyanins (SCs), plantacyanins (PLCs), and early nodulin-like proteins (ENODLs). The present study aimed to identify and characterise the PCs present in three distinct cotton species (Gossypium hirsutum, Gossyium arboreum, and Gossypium raimondii) through the identification of 98, 63, and 69 genes respectively. We grouped PCs into four clades by using bioinformatics analysis and sequence alignment, which exhibit variations in gene structure and motif distribution. PCs are distributed across all chromosomes in each of the three species, with varying numbers of exons per gene and multiple conserved motifs, and with a minimum of 1 and maximum of 11 exons found on one gene. Transcriptomic data and qRT-PCR analysis revealed that two highly differentiated PC genes were expressed at the fibre initiation stage, while three highly differentiated PCs were expressed at the fibre elongation stage. These findings serve as a foundation for further investigations aimed at understanding the contribution of this gene family in cotton fibre production.

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Section: Methodsmentioning

confidence: 99%

Identification and Characterization of Phytocyanin Family Genes in Cotton Genomes

Tufail

Yasir

Zuo

et al. 2023

Genes

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“…Furthermore, orthologue genes were identified by following the same procedure for Arabidopsis thaliana , Oryza sativa , and Glycine max . All collected sequences were checked using the NCBI Conserved Domain Database (CDD) [ 52 ] and the Pfam database [ 53 ] to confirm the presence of domains related to the SULTRs [ 54 ]. The physiochemical properties, including molecular weight (MW), instability index, isoelectric point (pI), and GRAVY value, of the SULTRs were predicted using the ProtParam tool [ 55 ].…”

Section: Methodsmentioning

confidence: 99%

Genome-Wide Characterization of the Sulfate Transporter Gene Family in Oilseed Crops: Camelina sativa and Brassica napus

Heidari

Hasanzadeh

Faraji

et al. 2023

Plants

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Sulfate transporters (SULTRs) are responsible for the uptake of sulfate (SO42−) ions in the rhizosphere by roots and their distribution to plant organs. In this study, SULTR family members in the genomes of two oilseed crops (Camelina sativa and Brassica napus) were identified and characterized based on their sequence structures, duplication events, phylogenetic relationships, phosphorylation sites, and expression levels. In total, 36 and 45 putative SULTR genes were recognized in the genomes of C. sativa and B. napus, respectively. SULTR proteins were predicted to be basophilic proteins with low hydrophilicity in both studied species. According to the observed phylogenetic relationships, we divided the SULTRs into five groups, out of which the SULTR 3 group showed the highest variation. Additionally, several duplication events were observed between the SULTRs. The first duplication event occurred approximately five million years ago between three SULTR 3.1 genes in C. sativa. Furthermore, two subunits were identified in the 3D structures of the SULTRs, which demonstrated that the active binding sites differed between C. sativa and B. napus. According to the available RNA-seq data, the SULTRs showed diverse expression levels in tissues and diverse responses to stimuli. SULTR 3 was expressed in all tissues. SULTR 3.1 was more upregulated in response to abiotic stresses in C. sativa, while SULTR 3.3 and SULTR 2.1 were upregulated in B. napus. Furthermore, SULTR 3 and SULTR 4.1 were upregulated in response to biotic stresses in B. napus. Additionally, the qPCR data showed that the SULTRs in C. sativa were involved in the plant’s response to salinity. Based on the distribution of cis-regulatory elements in the promoter region, we speculated that SULTRs might be controlled by phytohormones, such as ABA and MeJA. Therefore, it seems likely that SULTR genes in C. sativa have been more heavily influenced by evolutionary processes and have acquired further diversity. The results reveal new insights of the structures and functions of SULTRs in oilseed crops. However, further analyses, related to functional studies, are needed to uncover the role of SULTRs in the plants’ development and growth processes, as well as in their response to stimuli.

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“…Life represents a contradictory unity of growth and stress response. During the growth, development, and reproduction of plants they may suffer from a variety of abiotic and biotic stresses, such as drought, salinity, cold, heat, toxic metals, and diseases [ 1 , 2 ]. To adapt to changes in the natural environment, plants carry out the simultaneous regulation of growth and stress through a series of signal transduction.…”

Section: Introductionmentioning

confidence: 99%

Advances in Receptor-like Protein Kinases in Balancing Plant Growth and Stress Responses

Zhu

Feng

Jiao

et al. 2023

Plants

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Accompanying the process of growth and development, plants are exposed to ever-changing environments, which consequently trigger abiotic or biotic stress responses. The large protein family known as receptor-like protein kinases (RLKs) is involved in the regulation of plant growth and development, as well as in the response to various stresses. Understanding the biological function and molecular mechanism of RLKs is helpful for crop breeding. Research on the role and mechanism of RLKs has recently received considerable attention regarding the balance between plant growth and environmental adaptability. In this paper, we systematically review the classification of RLKs, the regulatory roles of RLKs in plant development (meristem activity, leaf morphology and reproduction) and in stress responses (disease resistance and environmental adaptation). This review focuses on recent findings revealing that RLKs simultaneously regulate plant growth and stress adaptation, which may pave the way for the better understanding of their function in crop improvement. Although the exact crosstalk between growth constraint and plant adaptation remains elusive, a profound study on the adaptive mechanisms for decoupling the developmental processes would be a promising direction for the future research.

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Natural lupeol level variation among castor accessions and the upregulation of lupeol synthesis in response to light

Cited by 17 publications

References 33 publications

Identification and Characterization of Phytocyanin Family Genes in Cotton Genomes

Identification and Characterization of Phytocyanin Family Genes in Cotton Genomes

Genome-Wide Characterization of the Sulfate Transporter Gene Family in Oilseed Crops: Camelina sativa and Brassica napus

Advances in Receptor-like Protein Kinases in Balancing Plant Growth and Stress Responses

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