Natural mixed infections on Phalaenopsis species by groundnut bud necrosis orthotospovirus, cymbidium mosaic virus and odontoglossum ringspot virus in India

Pant, R. P.; Basavaraj, Y. B.; Srivastava, Nishant; Bhattarai, Ajay; Kumar, Ashwani; Sailo, Ngurzampuii; Rampal,; Barman, Dipankar

doi:10.1007/s42360-021-00356-w

Cited by 3 publications

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References 16 publications

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“…These finding suggested that CymMV and ORSV are the most prevalent viruses of orchids in India. Similar results were also observed by many workers (Beetham and Moran 1988, Zettler et al 1990, Wong et al 1994, Pant et al 2010, 2021.…”

Section: Resultssupporting

confidence: 89%

“…Besides, these two viruses, some new viruses like orchid fleck virus (OFV; genus Dichorhavirus, family Rhabdoviridae), calanthe mild mosaic virus (CalMMV; genus Potyvirus, family Potyviridae), groundnut bud necrosis virus (GBNV; genus Orthotospovirus, family Tospoviridae) were also found infecting different orchid species/hybrids, thereby, posing a serious threat to orchid cultivation in India. Mixed infection of three viruses from Phalaenopsis orchid has also been reported from India recently (Pant et al 2021). Present investigation reports the associations of four viruses and molecular characterization of the coat protein (CP) gene of four ORSV isolates collected from Sikkim and Darjeeling hills.…”

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confidence: 83%

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Detection of orchid viruses and molecular characterization of odontoglossum ringspot virus (ORSV) isolates

et al. 2021

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During a survey (2018), 44 orchid samples collected from Sikkim and Darjeeling Hills of West Bengal. The samples were examined under electron microscope (EM) followed by DAC-ELISA and RT-PCR assays were performed. Electron microscopy showed the presence of rigid rods (300 × 18 nm), flexuous rods (475 nm × 13 nm and 800 nm × 12 nm), bacilliform or bullets shaped particles (40 × 100-140 nm) and enveloped quasi-spherical particles (80-110 nm). Based on the particle morphology, DAC-ELISA was performed using the antibodies specific to odontoglossum ringspot virus (ORSV), cymbidium mosaic virus (CymMV), calanthe mild mosaic virus (CalMMV) and groundnut bud necrosis virus (GBNV), which revealed the presence of four viruses namely CymMV, ORSV, GBNV and CalMMV. RT-PCR assays also confirmed the presence of four viruses using their specific primers. The cloning, sequencing and sequence analysis of the coat protein gene sequences of the four ORSV isolates was performed. The sequence analyses revealed up to 100% sequence identity among the ORSV isolates of this study and more than 99% identity with the different ORSV isolates available at NCBI GenBank database. The higher level of sequence homology among ORSV population suggests that they are under least evolutionary pressure. The occurrence of the multiple viruses in different orchids also reflects that the orchid cultivation is under major threat.

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Section: Resultssupporting

confidence: 89%

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confidence: 83%