Natural myocardial ECM patch drives cardiac progenitor based restoration even after scarring

Sarig, Udi; Sarig, Hadar; de-Berardinis, Elio; Chaw, Su-Yin; Nguyen, Evelyne Bao-Vi; Ramanujam, Vaibavi Srirangam; Vu, Thang Duc; Al-Haddawi, Muthafar; Liao, Susan; Seliktar, Dror; Kofidis, Theo; Boey, Freddy Yin Chiang; Venkatraman, Subbu S.; Machluf, Marcelle

doi:10.1016/j.actbio.2016.08.031

Cited by 75 publications

(70 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…[68] Altogether, these findings strongly suggest that our gel formulations not only provide the mechanical support needed to stop deterioration and preserve cardiac function, but also induce an ongoing biological process which possibly leads to tissue repair, and consequently, improved cardiac function.…”

Section: Discussionmentioning

confidence: 91%

Biohybrid cardiac ECM-based hydrogels improve long term cardiac function post myocardial infarction

et al. 2017

Self Cite

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 91%

Biohybrid cardiac ECM-based hydrogels improve long term cardiac function post myocardial infarction

et al. 2017

Self Cite

View full text Add to dashboard Cite

“…One study on a rat model with chronic myocardial infarction was repaired with an adult ventricular porcine dcECM that showed recruitment of host cardiac progenitors and CMs into the scaffold, vascularization penetrating the scaffold, as well as some positive remodeling responses (Sarig et al, 2016). Adult ventricular zebrafish dcECM also holds promising potential as a scaffold for mammalian cardiac regeneration.…”

Section: Natural Engineering In Vitro Approaches To Drive Cardiomyocymentioning

confidence: 99%

Naturally Engineered Maturation of Cardiomyocytes

Scuderi

Butcher

2017

Front. Cell Dev. Biol.

166

202

View full text Add to dashboard Cite

Ischemic heart disease remains one of the most prominent causes of mortalities worldwide with heart transplantation being the gold-standard treatment option. However, due to the major limitations associated with heart transplants, such as an inadequate supply and heart rejection, there remains a significant clinical need for a viable cardiac regenerative therapy to restore native myocardial function. Over the course of the previous several decades, researchers have made prominent advances in the field of cardiac regeneration with the creation of in vitro human pluripotent stem cell-derived cardiomyocyte tissue engineered constructs. However, these engineered constructs exhibit a functionally immature, disorganized, fetal-like phenotype that is not equivalent physiologically to native adult cardiac tissue. Due to this major limitation, many recent studies have investigated approaches to improve pluripotent stem cell-derived cardiomyocyte maturation to close this large functionality gap between engineered and native cardiac tissue. This review integrates the natural developmental mechanisms of cardiomyocyte structural and functional maturation. The variety of ways researchers have attempted to improve cardiomyocyte maturation in vitro by mimicking natural development, known as natural engineering, is readily discussed. The main focus of this review involves the synergistic role of electrical and mechanical stimulation, extracellular matrix interactions, and non-cardiomyocyte interactions in facilitating cardiomyocyte maturation. Overall, even with these current natural engineering approaches, pluripotent stem cell-derived cardiomyocytes within three-dimensional engineered heart tissue still remain mostly within the early to late fetal stages of cardiomyocyte maturity. Therefore, although the end goal is to achieve adult phenotypic maturity, more emphasis must be placed on elucidating how the in vivo fetal microenvironment drives cardiomyocyte maturation. This information can then be utilized to develop natural engineering approaches that can emulate this fetal microenvironment and thus make prominent progress in pluripotent stem cell-derived maturity toward a more clinically relevant model for cardiac regeneration.

show abstract

“…Based on this concept, Sarig et al developed a natural myocardial ECM patch for cardiac regeneration [136]. The bioactive patches, which were fabricated with decellularized porcine cardiac extracellular matrix (pcECM), were able to recruit cardiomyocyte progenitor cells (GATA4 + , c-kit + ) and myocytes (MYLC + , TRPI + ) in both acute and chronic MI models.…”

Section: Cardiovascular Regeneration Using Stem Cell Recruitment Smentioning

confidence: 99%

Strategies to develop endogenous stem cell-recruiting bioactive materials for tissue repair and regeneration

Pacelli

Basu

Whitlow

et al. 2017

Advanced Drug Delivery Reviews

132

View full text Add to dashboard Cite

A leading strategy in tissue engineering is the design of biomimetic scaffolds that stimulate the body’s repair mechanisms through the recruitment of endogenous stem cells to sites of injury. Approaches that employ the use of chemoattractant gradients to guide tissue regeneration without external cell sources are favored over traditional cell-based therapies that have limited potential for clinical translation. Following this concept, bioactive scaffolds can be engineered to provide a temporally and spatially controlled release of biological cues, with the possibility to mimic the complex signaling patterns of endogenous tissue regeneration. Another effective way to regulate stem cell activity is to leverage the inherent chemotactic properties of extracellular matrix (ECM)-based materials to build versatile cell-instructive platforms. This review introduces the concept of endogenous stem cell recruitment, and provides a comprehensive overview of the strategies available to achieve effective cardiovascular and bone tissue regeneration.

show abstract

Natural myocardial ECM patch drives cardiac progenitor based restoration even after scarring

Cited by 75 publications

References 42 publications

Biohybrid cardiac ECM-based hydrogels improve long term cardiac function post myocardial infarction

Biohybrid cardiac ECM-based hydrogels improve long term cardiac function post myocardial infarction

Naturally Engineered Maturation of Cardiomyocytes

Strategies to develop endogenous stem cell-recruiting bioactive materials for tissue repair and regeneration

Contact Info

Product

Resources

About