Natural phytosanitary products effects on Bacillus Thuringiensis SUBSP. Kurstaki (Berliner)

Lozano, Everton Ricardi; Alves, Luís Francisco Angeli; Martinelo, Leonardo; Fromentini, Marina Andressa; Marchese, Luiz Paulo Calixto; Pinto, Fabiana Gisele da Silva; Potrich, Michele; Neves, Pedro Manuel Oliveira Janeiro

doi:10.5433/1679-0359.2012v33supl1p2891

Cited by 7 publications

(11 citation statements)

References 11 publications

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“…The effects of the NPs on the toxicity of Btk S-1905 on A. gemmatalis in this study were not in agreement with those of Silva et al (2012), who did not observe an effect of Ecolife ® on the toxicity of Btk HD-1 in Dipel WP ® . This difference may be explained by the fact that in the present study, a purified bacterium was used at a lower concentration known to be effective (LC 50 ), while Silva et al (2012) used Btk HD-1 at the RC, half of the RC, and the twice the RC.…”

Section: Treatmentcontrasting

confidence: 99%

“…kurstaki HD-1 spores obtained from the commercial product Dipel WP ® and NPs. According to Silva et al (2012), these products significantly reduced the CFU ml −1 , regardless of concentration. The authors also observed a significant reduction in CFUs (24.0%) using the Pironim product at the RC, in contrast with the results of this study (3.8%) (table 2).…”

Section: Discussionmentioning

confidence: 93%

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Action of natural phytosanitary products onBacillus thuringiensissubsp.kurstakiS-1905

Lozano

Neves

Alves

et al. 2017

Bull. Entomol. Res.

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The objective of this study was to evaluate the effects of natural phytosanitary products (NPs) on spores and crystals of Bacillus thuringiensis subsp. kurstaki S-1905 (Btk S-1905). For the spore assay, NPs and bacteria were applied in combination and individually. For the combined application, Btk S-1905 + NP mixtures were inoculated on nutrient agar (NA), and for the separate applications, the NPs were spread on NA plates, which were later inoculated with the pathogen. The number of colony-forming units (CFU) per milliliter was quantified after 18 h of incubation. For the crystal protein degradation assay, the Btk S-1905 + NP mixtures were added to the diet of Anticarsia gemmatalis (Lepidoptera: Erebidae), and mortality was evaluated at the following time points: 12, 24, 48, and 72 h. Scanning electron microscopy and agarose gel electrophoresis were carried out. Biogermex and Ecolife® reduced the CFU ml-1 in both combined and separate applications. Biogermex, Ecolife®, and Planta Clean were antagonistic to the action of bacterial toxins, and no product affected the morphology or resulted in the degradation of the crystal proteins. The remaining products evaluated did not reduce the CFU ml-1 and had additive effect when combined with the crystal toxin.

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Section: Treatmentcontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 93%

Action of natural phytosanitary products onBacillus thuringiensissubsp.kurstakiS-1905

Lozano

Neves

Alves

et al. 2017

Bull. Entomol. Res.

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“…In addition, as any liquid biofertilizer nowadays, Soil-Set • has insecticide, insect-repellent, acaricide, and fungicide action (GALLO et al, 2002). This contrasts from the results with the product Soil-Set • applied on Anticarsia gemmatalis caterpillars (Lepidoptera: Noctuidae), whose mortality rate ranged between 0.8 and 3.2% (SILVA et al, 2012),…”

Section: Effect Of Alternative Phytosanitary Products On the Biology contrasting

confidence: 73%

Alternative phytosanitary products against to white muscardine in Bombyx mori L. (Lepidoptera: Bombycidae)

et al. 2017

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This work aimed to evaluate the effects of the products Biogermex®, Agro-mos®, Soil-Set® and Planta Clean® on the development of white muscardine in Bombyx mori. Products have been tested at two stages for effects on biology of caterpillars and on silk production (1), and then they were evaluated to know their potential in the prevention/control of white muscardine (2). Products were sprayed on mulberry leaves (preventive treatment) and also on the integument of silkworms (curative treatment). At the first stage, the products were applied on four consecutive days, in one of the daily feeds. Planta Clean® - curative treatment - and Soil-Set® - preventive treatment - caused mortality in 63.3% and 30% of silk worms, respectively. The fungus Beauveria bassiana was inoculated at the concentration 5 × 107 conidia/mL on the leaves and insects. The products were applied three hours after application on the fungus, in a single application, and also for four days in the other groups. A group of silkworms sprayed only with the fungus served as control. Biogermex® - curative treatment applied once and for four times - was the most effective, reducing mortality by 25 and 26.7%, respectively, as compared with the control group. None of the evaluated products acts preventively against white muscardine.

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“…Along with the possible effect of pH, the reduction of CFU mL -1 could also be related to the compounds in the extracts, which could also cause deleterious effects on the spores. According to Silva et al (2012) the secondary metabolites found in the extracts can prevent spore germination or destroy the bacterial membrane after germination. This could have been a possibility in this study.…”

Section: Activity Of Aqueous Plant Extracts On Crystals Of B Thuringmentioning

confidence: 99%

“…Bacillus thuringiensis may be used alone or associated with plant extracts for the control of insect pests (SILVA et al, 2012.). This association can be important, especially in situations that required control of different insects species and/or in various development stages.…”

Section: Introductionmentioning

confidence: 99%

Activity of plant aqueous extracts on Bacillus thuringiensis and their interactions on Anticarsia gemmatalis (Lepidoptera: Erebinae)

Vilani

Lozano

Potrich

et al. 2017

SCA

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The association of plant extracts and B. thuringiensis may be important in situations that control of different insects species is required and/or control of insects in various development stages. However, extracts may have different effects on B. thuringiensis, compromising their mode of action. Thus, the objective of this study was to evaluate the activity of aqueous plant extracts on Bacillus thuringiensis subsp. kurstaki (Btk) and their interactions on Anticarsia gemmatalis (Lepidoptera: Erebinae). Cherry plant extracts (Eugenia uniflora), pepper (Capsicum baccatum), trumpet (Brugmansia suaveolens) and grape japan (Hovenia dulcis), were used at 5% concentrations. For compatibility studies, the extracts were mixed with Thuricide® (Btk) and the parameters evaluated were Colony Formation Units (CFU mL-1) for spores and, the A. gemmatalis mortality for crystals. The cherry extracts, pepper, and japan grape completely inhibited the CFU ml-1 of Btk and the cherry extract alone negatively affected the toxicity of Btk crystals, with significantly lower mortality of A. gemmatalis (20.40%) than that observed in the control with only Btk (79.44%). In the association between cherry, pepper, and grape japan extracts with B. thuringiensis, the extracts exhibited a negative effect in the formation of CFU. The cherry extract demonstrated a negative effect on crystals action.

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Natural phytosanitary products effects on Bacillus Thuringiensis SUBSP. Kurstaki (Berliner)

Cited by 7 publications

References 11 publications

Action of natural phytosanitary products onBacillus thuringiensissubsp.kurstakiS-1905

Action of natural phytosanitary products onBacillus thuringiensissubsp.kurstakiS-1905

Alternative phytosanitary products against to white muscardine in Bombyx mori L. (Lepidoptera: Bombycidae)

Activity of plant aqueous extracts on Bacillus thuringiensis and their interactions on Anticarsia gemmatalis (Lepidoptera: Erebinae)

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