Natural Plasmids in a Swiss Fermented Milk and Colostrum Product assessed by Microbiome Array

Abstract

Natural plasmids are present in fermented foods ranging from yogurts to sourdoughs, sausages, kimchi or pickling crabs. In this study, we describe the natural plasmids present in a product obtained by fermentation of bovine milk and colostrum. We used a microarray consisting in a chip covered in short DNA sequences that are specific to target microorganisms for a total of approximately 12,000 species. As far as plasmids are concerned, the array yielded 48 iterations, each one corresponding to a unique plasmid target. We discuss the putative role of some of these plasmids in contributing to the known properties of probiotics in supporting health and longevity.

Significance of hydrophobic and charged sequence similarities in sodium-bile acid cotransporter and vitamin D-binding protein macrophage activating factor

“…We chose to study this protein because of its role in DBP-MAF function and we found that there is a high degree of similarity with the other two proteins, DBP-MAF and NTCP, with particular reference to stretches of hydrophobic aminoacids. Based on these evidence, we asked an independent laboratory to perform in vitro experiments to assess the binding of DBP-MAF to NAGAB_HUMAN using, as ligands, purified DBP-MAF and PMF (6,8).…”

Color Doppler Evaluation of Isovolumetric Relaxation Time and of Signals Arising from Axons of the Median Nerve as a Means to Evaluate Mitochondrial Functionality in the Context of Immunotherapy of Cancer and Chronic Conditions Associated with Mitochondrial Dysfunction

“…Similar, albeit not identical in size, couples can be observed for the signals circled in "B" and "C", whereas the signals in "D" and "E" do not show repetitive patterns. It is also interesting to notice that, if the signals in "A" are associated with mitochondrial quantum entanglement, they may also be indicative of the occurrence of a wormhole; this is a structure enabling non-conventional transfer of information between separate points in time and space, independently of spatial contiguity or direction of the arrow of time as we recently discussed in the context of virus-associated information transfer (Pacini and Ruggiero, 2019). The signals evidenced for the first time in Fig.…”

Experimental evidence for the role of natural radioactivity in influencing viability of commensal microorganisms

“…Such a biodiversity reproduces the complexity of the human microbiota, thus reconstituting an organ that is essential for human health (52). The probiotic blend contains plasmids that are known to transfer biological information between bacteria (30), and this feature is essential for exchanging biological information pertaining to radiation protection. In addition, the probiotic blend contains phages that are known to stimulate immunity, repair DNA and transfer biological information to human cells (31).…”

“…The blend contains also lyophilized kefir grains that are characterized by a complex composition. The unique microbial composition of this probiotic blend was fully described in two recent articles (30,31). Human consumption of this blend is associated with detoxification of non-metal toxicants (32), decrease of serum alpha-N-acetylgalactosaminidase (nagalase) activity (32,33), decrease of serum C-reactive protein (CRP) (33), decrease of markers specific for multiple myeloma (34), and decrease of viral load in Hepatitis B (35).…”