Natural polyelectrolyte films based on layer-by layer deposition of collagen and hyaluronic acid

Zhang, Jun; Senger, Bernard; Vautier, Dominique; Picart, Catherine; Schaaf, Pierre; Voegel, Jean‐Claude; Lavalle, Philippe

doi:10.1016/j.biomaterials.2004.08.019

Cited by 209 publications

(189 citation statements)

References 45 publications

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“…Through their ability to spontaneously organize into a well-defined structure without external instruction, these membranes have the potential to complement minimally invasive surgical procedures, which differ from other prefabricated collagen-HyA membranes. 5 Furthermore, these membranes have the potential to promote highly localized regeneration and support healing at interfaces between various types of tissues, such as in the bone-tendon junction. Moreover, collagen-HyA membranes could also be used to deliver other growth factors for different regenerative medicine targets such as their use as bioactive dressings for wound-healing applications or as adjuncts to enhance the bioactivity of current graft implants.…”

Section: Bmp-2-releasing Self-assembled Membranesmentioning

confidence: 99%

“…[1][2][3][4][5][6] For instance, collagen alone accounts for *30% of all vertebrate body proteins and more than 90% of the extracellular proteins in connective tissues. 7 The use of collagen-based scaffolds has been shown to be advantageous because of their inherent cell-binding sites, availability, accessibility, and structural strength.…”

Section: Introductionmentioning

confidence: 99%

“…16,17 With these advantages in mind, we have recently reported on the self-assembly between collagen and HyA into membrane structures. 18 Unlike previous studies that have taken advantage of these oppositely charged molecules to build up collagen-HyA membranes using a layer-by-layer multistep deposition process, 5 the collagen-HyA membranes described here are fabricated using a one-step procedure. 18 CollagenHyA membranes are self-assembled by simply layering an equal volume of collagen solution on top of the denser HyA solution.…”

Section: Introductionmentioning

confidence: 99%

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Osteogenic Potential of BMP-2-Releasing Self-Assembled Membranes

Chung

Chien

Aguado

et al. 2013

Tissue Engineering Part A

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We report here the use of novel self-assembling collagen-hyaluronic acid (HyA) membranes to deliver bone morphogenetic protein-2 (BMP-2) for orthopedic applications. Prior work has demonstrated that collagen-HyA membranes are formed initially through electrostatic interactions between the oppositely charged collagen and HyA molecules, and that membrane growth is driven by osmotic pressure imbalances between the collagen and HyA solutions. The purpose of this study was to investigate the potential of incorporating charged growth factors such as BMP-2 within the membrane for regenerative medicine applications. Membrane material properties, protein mass loss, and release kinetics of BMP-2, as well as biocompatibility and osteogenic potential in vitro and in vivo using a subcutaneous mouse model were assessed. Scanning electron microscopy and mechanical testing confirmed no loss of structural or mechanical integrity upon BMP-2 incorporation into the membranes. Slow and steady release of the growth factor was demonstrated with 17% of total loaded BMP-2 released over the course of 49 days. To test biocompatibility and osteogenic potential in vitro, human mesenchymal stem cells were cultured on collagen-HyA membranes and showed greater proliferation rates (for up to 28 days) on membranes without BMP-2, but a greater alkaline phosphatase activity and osteocalcin production on membranes releasing BMP-2. In vivo subcutaneous implantation of the membranes showed a minimal immune response with osteoblasts and mineral deposits present in the ectopic site for BMP-2-releasing membranes, further demonstrating the potential of the BMP-2-releasing membranes to induce osteogenic differentiation. This study presents a novel strategy to create self-assembled membranes using two biocompatible molecules that can deliver bioactive agents in a sustained manner to induce a local regenerative response.

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Section: Bmp-2-releasing Self-assembled Membranesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Osteogenic Potential of BMP-2-Releasing Self-Assembled Membranes

Chung

Chien

Aguado

et al. 2013

Tissue Engineering Part A

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“…These biomacromolecules form physically or chemically crosslinked networks, regulating the expression of phenotype of the osteoblast and supporting osteogenesis both in vitro and in vivo [23,24]. Therefore, many natural extracellular matrixes (ECM)-like macromolecules, such as hyaluronic acid (HA) or collagen, have been incorporated into the 3-D scaffolds for bone regeneration [24][25][26].…”

Section: Introductionmentioning

confidence: 99%

“…Col I, an important ECM component of native bone tissue, has been verified as possessing osteoprotective ability [24,25]. Moreover, it is positively charged in solution below isoelectric point [26], which allows Col I to be used as a polycation in a LBL assembly system, on which various polyanions can be adsorbed. In addition, HA is a major component of the extracellular matrix and of the synovial fluid, is also a negatively charged polyelectrolyte which has been used widely in a LBL assembly system [26,27].…”

Section: Introductionmentioning

confidence: 99%

LBL coating of type I collagen and hyaluronic acid on aminolyzed PLLA to enhance the cell-material interaction

Zhao¹,

Zhou²,

Li³

et al. 2014

Express Polym. Lett.

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Abstract. The aim of the present work is to assemble extracellular matrix components onto poly (L-lactic acid) (PLLA) films using layer-by-layer (LBL) depositing method to enhance the cell-material interaction. To introduce charges onto the hydrophobic and neutral PLLA surface so that the electronic assembly can be processed, poly (ethylene imine) (PEI) was covalently bonded to modify the PLLA films. Positively charged collagen I (Col I) was then deposited onto the aminolyzed PLLA film surface in a LBL assembly manner using hyaluronic acid (HA) as a negatively charged polyelectrolyte. The PEI modification efficiency was monitored via X-ray photoelectron spectroscopy (XPS) measurements. The results of Surface Plasmon Resonance (SPR) and Water contact angle (WCA) monitoring the LBL assemble process presented that the HA/Col I deposited alternately onto the PLLA surface. The surface topography of the films was observed by Atomic force microscope (AFM). In vitro osteoblast culture found that the presence of Col I layer greatly improved the cytocompatibility of the PLLA films in terms of cell viability, cell proliferation and Alkaline Phosphatase (ALP) expression. Furthermore, osteoblast extensions were found to be directed by contact guidance of the aligned Col I fibrils. Thus, these very flexible systems may allow broad applications for improve the bioactivity of polymeric materials, which might be a potential application for bone tissue engineering.

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Natural‐Based and Stimuli‐Responsive Polymers for Tissue Engineering and Regenerative Medicine

Oliveira

2014

Polymers in Regenerative Medicine

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Natural polyelectrolyte films based on layer-by layer deposition of collagen and hyaluronic acid

Cited by 209 publications

References 45 publications

Osteogenic Potential of BMP-2-Releasing Self-Assembled Membranes

Osteogenic Potential of BMP-2-Releasing Self-Assembled Membranes

LBL coating of type I collagen and hyaluronic acid on aminolyzed PLLA to enhance the cell-material interaction

Natural‐Based and Stimuli‐Responsive Polymers for Tissue Engineering and Regenerative Medicine

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