Natural product formation by plant cell biotechnology

Alfermann, A. Wilhelm; Petersen, Maike

doi:10.1007/bf00052176

Cited by 99 publications

(40 citation statements)

References 34 publications

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“…Thus, the large-scale production in vitro of bioactive compounds or extracts used as phytotherapics, pharmaceutical products, food additives and cosmetics should be encouraged because of their scientific, economical or ecological importance (1,2,4,5,11). However, the production in vitro of bioactive metabolites or extracts can be regarded as the result of the interaction of environmental conditions and the genotype of the cultured plant cells.…”

Section: Introductionmentioning

confidence: 99%

Comparative study of antibacterial and antifugal activity of callus culture and adult plants extracts from Alternanthera maritima (Amaranthaceae)

Salvador¹,

Pereira²,

França³

et al. 2004

Braz. J. Microbiol.

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The aim of this study was to evaluate the antibacterial and antifungal activity of callus culture (two different hormonal combination culture medium) and adult plants (two collect) extracts from Alternanthera maritima (Amaranthaceae) investigating the maintenance of antimicrobial activity in vivo and in vitro. The antibacterial and antifungal activity was determined by the agar-well diffusion method against thirty strains of microorganisms including Gram-positive and Gram-negative bacteria, yeasts and dermatophytes. All the organic crude extracts studied were bioactive. Extracts of aerial parts and roots of adult plants collected during the same period of years of 1995 and 1998 (Restinga de Maricá (RJ), collect 1 and 2) inhibited the growth of several microorganisms (bacteria, yeasts and dermatophytes) with inhibition halo between 6 and 20 mm. Plant cell callus culture extracts obtained from two culture conditions were also bioactive. Thus, the positive results suggest that the A. maritima extracts should be further studied to determine the bioactive chemical compounds as well as to understand the possible mechanisms of action and evaluate their toxicity looking toward a pharmaceutical employment.

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Section: Introductionmentioning

confidence: 99%

Comparative study of antibacterial and antifugal activity of callus culture and adult plants extracts from Alternanthera maritima (Amaranthaceae)

Salvador¹,

Pereira²,

França³

et al. 2004

Braz. J. Microbiol.

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“…They provide a way to study the biosynthesis of secondary metabolites and the factors that influence it giving some possibilities of controlled production. Detailed studies on the optimization of the culture medium, identification of the full range of plant secondary metabolites produced, stability of production and definition of biosynthetic pathways by in vitro cultures have been reported ( [1][2][3][4], and refs. therein).…”

Section: Introductionmentioning

confidence: 99%

Xanthone biosynthesis and accumulation in calli and suspended cells of Hypericum androsaemum

et al. 2000

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Calli and suspended cells of Hypericum androsaemum accumulated high levels of 1,3,5,6 and 1,3,6,7 oxygenated xanthones. The major compounds include simple oxygenated xanthones or derivatives with prenyl, pyran or methoxyl groups, four of them being new natural compounds. A hypothetical biosynthetic scheme is proposed based on the isolated compounds and statistical analysis. Xanthone accumulation was influenced greatly by medium factors, namely hormone supplementation. Calli grown with 4.5 mM a-naphtaleneacetic acid (NAA) +2.3 mM kinetin (KIN) had the highest specific xanthone production (1.3% biomass dry weight (DW)), whereas suspended cells grown in similar medium accumulated a lower amount (0.87% DW). Calli displayed a negative linear relationship between total xanthone accumulation and NAA concentration, in the range of 4.5 -22.5 mM. However, in this range the xanthone 1,3,6,7/1,3,5,6 ratio and the biomass production showed a positive linear relationship with NAA concentration. Substitution of 4.5 mM of NAA by the same molar amount of 2,4-dichlorophenoxyacetic acid (2,4-D), in the presence of 2.3 mM of KIN, caused a decrease in xanthone accumulation in calli. The use of N 6 -benzyladenine (BA) instead of KIN reduced xanthone production, independently of the auxin used. This effect was attenuated when both hormones were present.

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“…These factors have ensured the need to use novel biotechnological approaches for producing plant based chemicals naturally. Use of plant tissue culture systems is a better alternative for producing high-value secondary metabolites [65][66][67][68]70]. Plant cells exhibit the property of totipotency, i.e.…”

Section: Need Of Biotechnological Approachesmentioning

confidence: 99%

In vitro production of capsaicin through plant tissue culture

Swetnisha

2017

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Capsaicin, a secondary metabolite produced in capsicum, is in high demand in pharmaceutical industry because of its various medicinal properties. Currently, the supply of capsaicin depends upon its extraction from capsicum fruits. This limits the production of capsaicin as it depends upon agricultural produce. The current review has compiled information from various literature published on chemistry and importance of capsaicin along with its method of production. It also reviews the process of in vitro production of capsaicin through plant tissue culture, strategies of increasing capsaicin accumulation and its advantages over extraction from fruits and artificial synthesis.

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Natural product formation by plant cell biotechnology

Cited by 99 publications

References 34 publications

Comparative study of antibacterial and antifugal activity of callus culture and adult plants extracts from Alternanthera maritima (Amaranthaceae)

Comparative study of antibacterial and antifugal activity of callus culture and adult plants extracts from Alternanthera maritima (Amaranthaceae)

Xanthone biosynthesis and accumulation in calli and suspended cells of Hypericum androsaemum

In vitro production of capsaicin through plant tissue culture

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