Natural Production of Organohalide Compounds in the Environment

Field, James A.

doi:10.1007/978-3-662-49875-0_2

Cited by 28 publications

(20 citation statements)

References 119 publications

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“…S13E, F). Such resistance of marine OHRB to oxygen may enable them to occupy niches close to halogenating organisms/enzymes that nearly all use oxygen or peroxides as reactants [71]. For instance, the marine sponge A. aerophoba from which D. spongiiphila AA1 T was isolated [20] harbors bacteria with a variety of FADH 2dependent halogenases [72], and produces a variety of brominated secondary metabolites [54].…”

Section: Potential Oxygen Defense In Desulfoluna Strainsmentioning

confidence: 99%

Organohalide-respiring Desulfoluna species isolated from marine environments

Peng

Goris

et al. 2020

The ISME Journal

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The genus Desulfoluna comprises two anaerobic sulfate-reducing strains, D. spongiiphila AA1 T and D. butyratoxydans MSL71 T , of which only the former was shown to perform organohalide respiration (OHR). Here we isolated a third strain, designated D. spongiiphila strain DBB, from marine intertidal sediment using 1,4-dibromobenzene and sulfate as the electron acceptors and lactate as the electron donor. Each strain harbors three reductive dehalogenase gene clusters (rdhABC) and corrinoid biosynthesis genes in their genomes, and dehalogenated brominated but not chlorinated organohalogens. The Desulfoluna strains maintained OHR in the presence of 20 mM sulfate or 20 mM sulfide, which often negatively affect other organohalide-respiring bacteria. Strain DBB sustained OHR with 2% oxygen in the gas phase, in line with its genetic potential for reactive oxygen species detoxification. Reverse transcription-quantitative PCR revealed differential induction of rdhA genes in strain DBB in response to 1,4-dibromobenzene or 2,6-dibromophenol. Proteomic analysis confirmed expression of rdhA1 with 1,4-dibromobenzene, and revealed a partially shared electron transport chain from lactate to 1,4-dibromobenzene and sulfate, which may explain accelerated OHR during concurrent sulfate reduction. Versatility in using electron donors, de novo corrinoid biosynthesis, resistance to sulfate, sulfide and oxygen, and concurrent sulfate reduction and OHR may confer an advantage to marine Desulfoluna strains.

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Section: Potential Oxygen Defense In Desulfoluna Strainsmentioning

confidence: 99%

Organohalide-respiring Desulfoluna species isolated from marine environments

Peng

Goris

et al. 2020

The ISME Journal

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“…CF is synthetically produced in chemical industries for various applications [4]. However, overall anthropogenic sources were estimated to contribute to less than 10% of the global CF production of 700-820 Gg/y [5]. Natural CF emissions have been reported from numerous terrestrial and aquatic environments such as forest soils [6][7][8][9], rice fields [10], groundwater [11], oceans [12], and hypersaline lakes [13,14].…”

Section: Introductionmentioning

confidence: 99%

Metagenomic- and Cultivation-Based Exploration of Anaerobic Chloroform Biotransformation in Hypersaline Sediments as Natural Source of Chloromethanes

Peng

Bosma

et al. 2020

Microorganisms

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Chloroform (CF) is an environmental contaminant that can be naturally formed in various environments ranging from forest soils to salt lakes. Here we investigated CF removal potential in sediments obtained from hypersaline lakes in Western Australia. Reductive dechlorination of CF to dichloromethane (DCM) was observed in enrichment cultures derived from sediments of Lake Strawbridge, which has been reported as a natural source of CF. No CF removal was observed in abiotic control cultures without artificial electron donors, indicating biotic CF dechlorination in the enrichment cultures. Increasing vitamin B12 concentration from 0.04 to 4 µM in enrichment cultures enhanced CF removal and reduced DCM formation. In cultures amended with 4 µM vitamin B12 and 13C labelled CF, formation of 13CO2 was detected. Known organohalide-respiring bacteria and reductive dehalogenase genes were neither detected using quantitative PCR nor metagenomic analysis of the enrichment cultures. Rather, members of the order Clostridiales, known to co-metabolically transform CF to DCM and CO2, were detected. Accordingly, metagenome-assembled genomes of Clostridiales encoded enzymatic repertoires for the Wood-Ljungdahl pathway and cobalamin biosynthesis, which are known to be involved in fortuitous and nonspecific CF transformation. This study indicates that hypersaline lake microbiomes may act as a filter to reduce CF emission to the atmosphere.

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“…Polyhalogenated organohalogens usually have low solubility in water and persistent under oxic conditions (Field andSierra-Alvarez 2008, Nikel et al 2013). Reductive dehalogenation is the only documented microbial process for dehalogenation of these organohalogens that has been observed in suboxic and anoxic environments such as subsurface soil, groundwater and marine environments (Atashgahi et al 2016).…”

Section: Reductive Dehalogenationmentioning

confidence: 99%

“…S3.12E, F). Such resistance of marine OHRB to oxygen may enable them to occupy niches close to halogenating organisms/enzymes that nearly all use oxygen or peroxides as reactants (Field 2016). For instance, the marine sponge A. aerophoba from which D. spongiiphila AA1 T was isolated (Ahn et al 2009) harbors bacteria with a variety of FADH2dependent halogenases (Bayer et al 2013), and produces a variety of brominated secondary metabolites (Turon et al 2000).…”

Section: Potential Oxygen Defense In Desulfoluna Strainsmentioning

confidence: 99%

“…CF is synthetically produced in chemical industries as an anesthetic, as an intermediate for the production of refrigerants, and as a degreasing agent and fumigant (ATSDR 1997). However, anthropogenic sources were estimated to contribute to less than 10% of the annual 700,000-820,000 tons global CF production (Field 2016). Natural CF emissions have been reported from numerous terrestrial and aquatic environments such as forest soils (Albers et al 2010, Breider et al 2013, Osswald et al 2016, rice fields (Khalil et al 1998), groundwater (Hunkeler et al 2012), oceans (Nightingale 1991), and hypersaline lakes (Ruecker et al 2014, Weissflog et al 2005.…”

Section: Introductionmentioning

confidence: 99%

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Microbial transformation of organic and inorganic halogen compounds

Peng¹

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Haloalkanoates are environmental pollutants that can be degraded aerobically by microorganisms producing hydrolytic dehalogenases. However, there is lack of information about anaerobic degradation of haloalkanoates. Genome analysis of Pseudomonas chloritidismutans AW-1 T , a facultative anaerobic chlorate-reducing bacterium, showed presence of two putative haloacid dehalogenase genes, the L-DEX gene and dehI, encoding an L-2-haloacid dehalogenase (L-DEX) and a halocarboxylic acid dehydrogenase (DehI). Hence, we studied concurrent degradation of haloalkanoates and chlorate as a yet unexplored trait of strain AW-1 T. The deduced amino acid sequences of L-DEX and DehI revealed 33−37% and 26−86% similarities with biochemically/structurally characterized L-DEX and D-, DL-2haloacid dehalogenase enzymes, respectively. Physiological experiments confirmed that strain AW-1 T can grow on chloroacetate, bromoacetate and both Land D-α-halogenated propionates with chlorate as an electron acceptor. Interestingly, growth and haloalkanoates degradation were generally faster with chlorate as an electron acceptor than with oxygen. In line with this, analyses of L-DEX and DehI dehalogenase activities using cell free extract (CFE) of strain AW-1 T grown on DL-2-chloropropionate under chlorate-reducing condition showed up to 3.5-fold higher dehalogenase activity than the CFE obtained from cells grown on DL-2chloropropionate under aerobic condition. Reverse transcription quantitative PCR showed that the L-DEX gene was expressed constitutively independent of the electron donor (haloalkanoates or acetate) or acceptor (chlorate or oxygen), whereas expression of dehI was induced by haloalkanoates. Concurrent degradation of organic and inorganic halogenated compounds by strain AW-1 T represents a unique metabolic capacity in a single bacterium, providing a new piece in the puzzle of the microbial halogen cycle.

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Natural Production of Organohalide Compounds in the Environment

Cited by 28 publications

References 119 publications

Organohalide-respiring Desulfoluna species isolated from marine environments

Organohalide-respiring Desulfoluna species isolated from marine environments

Metagenomic- and Cultivation-Based Exploration of Anaerobic Chloroform Biotransformation in Hypersaline Sediments as Natural Source of Chloromethanes

Microbial transformation of organic and inorganic halogen compounds

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