Natural products as a novel source for antileishmanial drug development

Gouri, Vinita; Pandey, Satish Chandra; Joshi, Diksha; Pande, Veni; Upreti, Shobha; Samant, Mukesh

doi:10.1016/b978-0-12-822800-5.00011-1

Cited by 3 publications

(2 citation statements)

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“…[ 33 ] The ethnomedical evaluation for the leishmanicidal activity of the P. abrotanoides has been recently confirmed by methanolic and ethanolic extract of its roots in vitro . [ 34 ] Also, its phytochemical evaluations showed the presence of terpenoids such as diterpenes, sesquiterpenes, and monoterpenes, e.g., camphene, 1, 8-Cineol, myrcene, pinene, camphor, caryophyllene, and humulene in plant extract. [ 35 ] On the other hand, the effect of terpenoid compounds against different species of Leishmania has been sufficiently strong to be considered as lead compounds for drug development.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Study of the Leishmanicidal Activity of Perovskia Abrotanoides Terpenoid-Rich Fractions Against Leishmania Major (MRHO/IR/75/ER)

Sadeghi Dinani,

Emarati Noushabadi,

Namdar

et al. 2023

Advanced Biomedical Research

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Background: Cutaneous leishmaniasis (CL) is an ulcerative skin disease caused by some species of the genus Leishmania . Evidence shows that Perovskia abrotanoides is an important herbal medicine against Leishmania . This study was conducted to investigate the killing effect of terpenoid-rich fractions on promastigotes of L. major (MRHO/IR/75/ER). Material and Method: The eluates of reverse phased medium pressure liquid chromatography (RP-MPLC) of the extract were subjected to thin-layer chromatography (TLC) and categorized into six final fractions. Primary proton nuclear magnetic resonance (H-NMR) spectroscopy confirmed fractions' nature. Fractions 4, 5, and 6 (F4, F5, F6) were identified as terpenoid-rich content. Two concentrations of 50 and 100 μg/ml were prepared to test leishmanicidal activity. Followed by treating promastigotes of L. major by the fractions in incubation times of 12, 24, and 48 hours, their viability was determined using a cell proliferation MTS ((3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay. Result: F4, F5, and F6 showed significant killing activity on promastigotes of L. major in a concentration-dependent manner. The viability of promastigotes was significantly reduced at a concentration of 100 μg/ml compared to 50 μg/ml (P-value <0.05). Also, over time a significant decreasing trend in the viability of promastigotes confirmed the time-dependent manner of the fractions (P-value <0.01). Furthermore, F5 had the highest leishmanicidal activity at the first incubation time compared with other fractions. Conclusion: Terpenoid-rich fractions of the P. abrotanoides have a leishmanicidal activity that depends on time and concentration. Among them, F5 has the highest potency that may contain potent terpenoid constituents.

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Section: Discussionmentioning

confidence: 99%

In Vitro Study of the Leishmanicidal Activity of Perovskia Abrotanoides Terpenoid-Rich Fractions Against Leishmania Major (MRHO/IR/75/ER)

Sadeghi Dinani,

Emarati Noushabadi,

Namdar

et al. 2023

Advanced Biomedical Research

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“…In recent years, there has been growing interest in natural products such as plants and their derivatives for the treatment of leishmaniasis [7][8][9]. Antileishmanial activity of various plants has been attributed to the presence of compounds such as favonoids, alkaloids, steroids, triterpenoids, naphthoquinones, quinones, terpenes, lignans, and saponins [10].…”

Section: Introductionmentioning

confidence: 99%

Topically Applied Luteolin /Quercetin-Capped Silver Nanoparticle Ointment as Antileishmanial Composite: Acceleration Wound Healing in BALB/c Mice

Ebrahimzadeh

Karamian

Abedi

et al. 2023

Advances in Materials Science and Engineering

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Cutaneous leishmaniasis is a major health problem in several parts of the world. However, existing drugs have various therapeutic effects on different Leishmania species and also cause many side effects. This study focuses on the use of the aqueous extract of quercetin in producing silver nanoparticles for the experimental treatment of cutaneous leishmaniasis in laboratory mice. Silver nanoparticles (AgNPs) were synthesized using quercetin and characterized by UV-Vis spectroscopy, DLS, FTIR, and FESEM. We investigated the antileishmanial activity of green synthesized AgNPs in vitro and in vivo. Synthesized AgNPs from quercetin macromolecules showed promising antileishmanial activity at an IC50 of 125 μg/ml against promastigotes in vitro. In the in vivo study, L. major-infected BALB/c mice were treated topically with luteolin/quercetin-capped silver nanoparticle (AgNPQct) ointment for 21 consecutive days. As a result, this ointment reduced the inflammatory response compared to the untreated group ( p < 0.05). The size of lesions in the luteolin/AgNPQct group showed a slight increase compared to other groups ( p < 0.01). Biogenic silver nanoparticles along with the luteolin macromolecule can be an effective and reliable candidate for the design of antileishmanial drugs in the future.

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Ficus carica hairy roots

Khademvatan

Amani

Mohebodini

et al. 2022

Asian Pacific Journal of Tropical Medicine

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Objective: To investigate the biochemical capacity, and in vitro inhibitory effects of hairy roots from two cultivars of Ficus carica L. (Sabz and Siah) on Leishmania major promastigotes and amastigotes. Methods: In the hairy roots, the activity of antioxidant enzymes compared to normal leaves and roots, and the presence of some phenolic compounds in comparison with fruits were investigated. The IC50 values of hairy roots in promastigotes was determined by tetrazolium-dye 3-(4, 5-dimethylthiazol-2- yl)-2, 5-diphenyltetrazolium bromide and trypan blue assays. By calculating the infectivity index of peripheral blood mononuclear cells (PBMCs), the leishmanicidal activity (IC50 values) of hairy roots for amastigotes was estimated. The effects of hairy roots (IC50 values) treatment on the levels of IFN-γ and iNOS expression, intracellular reactive oxygen species, and iNOS protein expression in infected-PBMCs were determined. Results: Based on antioxidant enzyme assays and high performance liquid chromatography analysis, hairy roots exhibited high antioxidant capacity and contained high levels of phenolic compounds. According to the results of tetrazolium-dye 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide and trypan blue assays, the hairy root extracts of both cultivars showed considerable dose-dependent inhibitory effects against Leishmania major promastigotes. Depending on the concentration and exposure time, treatment of infected-PBMCs with hairy root extracts caused the generation of a significant reactive oxygen species, up- regulation of IFN-γ and iNOS genes expression, and high value of iNOS protein compared to controls. Conclusions: The findings of this study suggest that the hairy roots of Ficus carica can be considered as a promising natural source of antileishmanial agents.

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Natural products as a novel source for antileishmanial drug development

Cited by 3 publications

References 69 publications

In Vitro Study of the Leishmanicidal Activity of Perovskia Abrotanoides Terpenoid-Rich Fractions Against Leishmania Major (MRHO/IR/75/ER)

In Vitro Study of the Leishmanicidal Activity of Perovskia Abrotanoides Terpenoid-Rich Fractions Against Leishmania Major (MRHO/IR/75/ER)

Topically Applied Luteolin /Quercetin-Capped Silver Nanoparticle Ointment as Antileishmanial Composite: Acceleration Wound Healing in BALB/c Mice

Ficus carica hairy roots

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