Natural Products from Plant-Associated Microorganisms:  Distribution, Structural Diversity, Bioactivity, and Implications of Their Occurrence

Gunatilaka, A. A. Leslie

doi:10.1021/np058128n

Cited by 954 publications

(584 citation statements)

References 130 publications

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“…11,12,14 It is widely believed that the potential of secondary metabolites with biological activities from endophytic actinomycetes is just as great as that achieved from soil actinomycetes. [16][17][18][19][20] As results of our exploration, we have already found several novel compounds from endophytic actinomycetes. In a similar vein, many new genera of actinomycetes, such as Allonocardiopsis from the fruit of a medical plant, 21 Actinophytocola from Thai glutinous roots of rice plants, 22 Plantactinospora from root of a medical plant, 23 had been discovered and promising endophytes with unknown function may arise from plants.…”

Section: Introductionmentioning

confidence: 92%

Endophytic actinomycetes: promising source of novel bioactive compounds

2017

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Endophytic actinomycetes associated with plant roots are a relatively untapped source of potential new bioactive compounds. This is becoming increasingly important, as the returns from discovery research on soil-dwelling microbes, have been continuously diminishing. We have isolated more than 1000 strains of actinomycetes from plant roots in our search for novel bioactive compounds, identified and assayed their bioactive metabolites, as well as investigated their biosynthetic genes for generating secondary metabolites. This has resulted in the discovery of several interesting compounds. Creation of plant root clone libraries enabled us to confirm that we had, indeed, isolated endophytes. In this paper, we introduce our approach to this promising line of research, incorporating data from other publications, and illustrate the potential that endophytic actinomycetes offer as a new source of novel lead compounds.

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Section: Introductionmentioning

confidence: 92%

Endophytic actinomycetes: promising source of novel bioactive compounds

2017

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“…It is generally recognized that endophytes represent an important and largely untapped reservoir of unique chemical structures that have been modified through evolution (Gunatilaka 2006;Kharwar et al 2011) and exhibit the capability to produce the same functional compounds as their hosts, some examples include taxol (Stierle et al 1993(Stierle et al , 1995, podophyllotoxin , hypericin (Kusari et al 2008), and azadirachtin (Findlay et al 1997). In 2005, Puri et al reported the first discovery of CPT-producing endophytic fungus Entrophospora infrequens from the inner bark of N. foetida, which set the stage for a more comprehensive examination of other plants for the presence of CPT-producing endophytes Rehman et al 2008Rehman et al , 2009Kusari et al 2009;Shweta et al 2010Shweta et al , 2013Gurudatt et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Camptothecin-producing endophytic fungus Trichoderma atroviride LY357: isolation, identification, and fermentation conditions optimization for camptothecin production

Xiang

Xing

Chen

et al. 2013

Appl Microbiol Biotechnol

154

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Camptothecin (CPT), the third largest anticancer drug, is produced mainly by Camptotheca acuminata and Nothapodytes foetida. CPT itself is the starting material for clinical CPT-type drugs, but the plant-derived CPT cannot support the heavy demand from the global market. Research efforts have been made to identify novel sources for CPT. In this study, three CPT-producing endophytic fungi, Aspergillus sp. LY341, Aspergillus sp. LY355, and Trichoderma atroviride LY357, were isolated and identified from C. acuminata. Most CPT produced by these fungi was found in the fermentation broth, and their corresponding CPT yields were 7. 93, 42.92, and 197.82 μg l −1 , respectively. The CPT-producing capability of LY341 and LY355 was completely lost after repeat subculturing. A substantial decrease of CPT production was also observed in the second generation of LY357. However, a stable and sustainable production of CPT was found from the second generation through the eighth generation of LY357. The fermentation medium, time, pH, temperature, and agitation rate were optimized for CPT production. Methyl jasmonate and XAD16 were proven to be an optimum elicitor and adsorbent resin, respectively, in view of that CPT yield was increased 3.4-and 11-fold through their use. A 50-to 75-fold increase of CPT yield was obtained when the optimized fermentation conditions, elicitor, and adsorbent resin were combined and applied to the culture of the seventh and eighth generations of LY357, and the highest CPT yield was 142.15 μg l −1 . The CPTproducing T. atroviride LY357 paves a potential to uncover the mysteries of CPT biosynthesis.

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“…Endophytic fungi living within plant tissues have proven to be a promising source for the production of structurally novel and pharmacologically active secondary metabolites [1]. As a result of our ongoing efforts to identify new compounds from endophytes, new metabolites were reported from strains of Xylaria sp.…”

Section: Introductionmentioning

confidence: 99%

Fusaristatins A and B, Two New Cyclic Lipopeptides from an Endophytic Fusarium sp.

et al. 2007

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Two new cyclic lipopeptides, fusaristatins A (1) and B (2) were isolated from rice cultures of a Fusarium sp. YG-45 in the course of a screening of endophytic fungi. Their structures of 1 and 2 were determined by spectroscopic methods. 2 showed a moderate inhibitory effect on topoisomerases I (IC 50 : 73 mM) and II (IC 50 : 98 mM) without cleavable complexes. Furthermore, 1 and 2 showed the growth-inhibitory activity toward lung cancer cells LU 65 with IC 50 values of 23 and 7 mM, respectively.

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Natural Products from Plant-Associated Microorganisms: Distribution, Structural Diversity, Bioactivity, and Implications of Their Occurrence

Cited by 954 publications

References 130 publications

Endophytic actinomycetes: promising source of novel bioactive compounds

Endophytic actinomycetes: promising source of novel bioactive compounds

Camptothecin-producing endophytic fungus Trichoderma atroviride LY357: isolation, identification, and fermentation conditions optimization for camptothecin production

Fusaristatins A and B, Two New Cyclic Lipopeptides from an Endophytic Fusarium sp.

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