Natural products of traditional Chinese medicine treat atherosclerosis by regulating inflammatory and oxidative stress pathways

Meng, Tianwei; Li, Xinghua; Li, Chengjia; Liu, Jiawen; Chang, Hong; Jiang, Nan; Li, Jiarui; Zhou, Yonggang; Liu, Zhiping

doi:10.3389/fphar.2022.997598

Cited by 14 publications

(7 citation statements)

References 112 publications

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“…When reperfusion injury occurs, the expression of pro-inflammatory factors, adhesive molecules, cytokines, and chemokines can also be up-regulated and then induce cell apoptosis. Our results also suggest that serum TNF-α, IL-6 and IL-1β levels were immediately upregulated at the beginning of reperfusion and subsequently decreased, but the levels of these inflammatory cytokines still remained higher than those in the sham group until 24 h. Many studies suggest that TLR4 signaling pathway plays an important role in the transmission of inflammatory signals [ 32 , 33 ]. A large number of pro-inflammatory factors, chemokines and adhesion factors interact with TLR4 to promote the development of the disease [ 34 ].…”

Section: Discussionsupporting

confidence: 62%

Evaluation of time-dependent phenotypes of myocardial ischemia-reperfusion in mice

Meng,

Yuan,

Zhou

et al. 2023

Aging

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Background: A mouse model of myocardial ischemia-reperfusion (I/R) is widely used to study myocardial ischemia-reperfusion injury (I/RI). However, few studies focus on the direct comparison of the extent of pathological events resulting from variant durations of ischemia and reperfusion process. Methods: A mouse model of I/RI was established by ligation and perfusion of the left anterior descending coronary artery (LAD), and the dynamic changes were recorded by electrocardiogram at different stages of I/R. Subsequently, reperfusion duration was used as a variable to directly compare the phenotypes of different myocardial injury degrees induced by 3 h, 6 h and 24 h reperfusion from myocardial infarct size, myocardial apoptosis, myocardial enzyme, and inflammatory cytokine levels. Results: All mice subjected to myocardial I/R surgery showed obvious myocardial infarction, extensive myocardial apoptosis, dynamic changes in serum myocardial enzyme and inflammatory cytokines, at least for the first 24 h of reperfusion. The infarct size and apoptosis rates gradually increased with the extension of reperfusion time. The peaks of serum myocardial enzyme and inflammatory cytokines occurred at 6 h and 3 h of reperfusion, respectively. We also established I/R mice models with 30 and 60 mins of ischemia. After 21 days of remodeling, longer periods of ischemia increased the degree of fibrosis and reduced cardiac function. Conclusions: In summary, we conclude that reperfusion durations of 3 h, 6 h, and 24 h induces different injury phenotypes in ischemia-reperfusion mouse model. At the same time, the ischemia duration before reperfusion also affects the degree of cardiac remodeling.

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Section: Discussionsupporting

confidence: 62%

Evaluation of time-dependent phenotypes of myocardial ischemia-reperfusion in mice

Meng,

Yuan,

Zhou

et al. 2023

Aging

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“…The JAK–STAT pathway is involved in almost all immunomodulatory processes and is a common pathway for a variety of cytokines and growth factors ( 78 ). IL-17 and IFN-γ activate TYK2 and JAK1 heterodimers.…”

Section: Iκbζ Is Induced Via Inflammatory Signal P...mentioning

confidence: 99%

The central inflammatory regulator IκBζ: induction, regulation and physiological functions

Feng

Chen

et al. 2023

Front. Immunol.

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IκBζ (encoded by NFKBIZ) is the most recently identified IkappaB family protein. As an atypical member of the IkappaB protein family, NFKBIZ has been the focus of recent studies because of its role in inflammation. Specifically, it is a key gene in the regulation of a variety of inflammatory factors in the NF-KB pathway, thereby affecting the progression of related diseases. In recent years, investigations into NFKBIZ have led to greater understanding of this gene. In this review, we summarize the induction of NFKBIZ and then elucidate its transcription, translation, molecular mechanism and physiological function. Finally, the roles played by NFKBIZ in psoriasis, cancer, kidney injury, autoimmune diseases and other diseases are described. NFKBIZ functions are universal and bidirectional, and therefore, this gene may exert a great influence on the regulation of inflammation and inflammation-related diseases.

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“…Traditional Chinese medicine is extensively used in clinical settings due to its minimal toxicity and low side effects [87]. In a clinical study, it was found that the Yiqi Huaju Qingli formula had a positive effect on patients with metabolic syndrome.…”

Section: Discussionmentioning

confidence: 99%

Huaju Xiaoji Formula Regulates ERS-lncMGC/miRNA to Enhance the Renal Function of Hypertensive Diabetic Mice with Nephropathy

Zhang,

Fu,

Zhou

et al. 2024

Journal of Diabetes Research

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Background. Better therapeutic drugs are required for treating hypertensive diabetic nephropathy. In our previous study, the Huaju Xiaoji (HJXJ) formula promoted the renal function of patients with diabetes and hypertensive nephropathy. In this study, we investigated the therapeutic effect and regulation mechanism of HJXJ in hypertensive diabetic mice with nephropathy. Methods. We constructed a mouse hypertensive diabetic nephropathy (HDN) model by treating mice with streptozotocin (STZ) and nomega-nitro-L-arginine methyl ester (LNAME). We also constructed a human glomerular mesangial cell (HGMC) model that was induced by high doses of sugar (30 mmol/mL) and TGFβ1 (5 ng/mL). Pathological changes were evaluated by hematoxylin and eosin (H&E) staining, periodic acid Schiff (PAS) staining, and Masson staining. The fibrosis-related molecules (TGFβ1, fibronectin, laminin, COL I, COL IV, α-SMA, and p-smad2/3) were detected by enzyme-linked immunosorbent assay (ELISA). The mRNA levels and protein expression of endoplasmic reticulum stress, fibrosis molecules, and their downstream molecules were assessed using qPCR and Western blotting assays. Results. Administering HJXJ promoted the renal function of HDN mice. HJXJ reduced the expression of ER stress makers (CHOP and GRP78) and lncMGC, miR379, miR494, miR495, miR377, CUGBP2, CPEB4, EDEM3, and ATF3 in HDN mice and model HGMCs. The positive control drugs (dapagliflozin and valsartan) also showed similar effects after treatment with HJXJ. Additionally, in model HGMCs, the overexpression of CHOP or lncMGC decreased the effects of HJXJ-M on the level of fibrosis molecules and downstream target molecules. Conclusion. In this study, we showed that the HJXJ formula may regulate ERS-lncMGC/miRNA to enhance renal function in hypertensive diabetic mice with nephropathy. This study may act as a reference for further investigating whether combining HJXJ with other drugs can enhance its therapeutic effect. The findings of this study might provide new insights into the clinical treatment of hypertensive diabetic nephropathy with HJXJ.

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Natural products of traditional Chinese medicine treat atherosclerosis by regulating inflammatory and oxidative stress pathways

Cited by 14 publications

References 112 publications

Evaluation of time-dependent phenotypes of myocardial ischemia-reperfusion in mice

Evaluation of time-dependent phenotypes of myocardial ischemia-reperfusion in mice

The central inflammatory regulator IκBζ: induction, regulation and physiological functions

Huaju Xiaoji Formula Regulates ERS-lncMGC/miRNA to Enhance the Renal Function of Hypertensive Diabetic Mice with Nephropathy

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