All types of stones have been used as building stones, depending on their durability, visual harmony with the intended construction and availability. In the Hellenistic period, as in other periods, tuff was preferred as a building stone due to its convenience -it was easy to extract, transport and use for building. In the present study, three ancient quarries that were the possible tuff sources for the Apollon Smintheus Temple in Ç anakkale are investigated by comparing the durability properties of stones in the temple and in the quarries. These properties are determined using physical and physico-mechanical tests, comparing fresh and artificially weathered samples. Microstructural and elemental correlations were found using optical microscopy, stereomicroscopy, X-ray diffraction, scanning electron microscopy, methylene blue adsorption and X-ray fluorescence analyses. The results indicate that temple tuffs and two of three quarries have similar geological engineering and microstructural properties with the strong claim that those two quarries could have been the source of building stone for the temple.