Natural transovarial transmission of dengue virus 4 in Aedes aegypti from Cuiabá, State of Mato Grosso, Brazil

Cruz, Lucinéia Claudia de Toni Aquino da; Serra, Otacília Pereira; Santos, Fábio Alexandre Leal dos; Ribeiro, Ana Lúcia Maria; Slhessarenko, Renata Dezengrini; Santos, Marina Atanaka dos

doi:10.1590/0037-8682-0264-2014

Cited by 33 publications

(25 citation statements)

References 41 publications

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“…This is corroborated by Baak-Baak et al 23 , who found 1.2% (2/166) positivity for dengue virus in churches. Meanwhile, in Belo Horizonte, the State capital of Minas Gerais, Eiras et al 24 found 4.9% (4/82 pools) and 8.5% (7/82 pools) infectivity to dengue virus and Zika virus, respectively, at the university Moreover, the possibility of transovarian transmission of dengue 25 , chikungunya 26 and Zika 27,28 in Ae. aegypti shows that a varied percentage of mosquitoes can be infected with these viruses.…”

Section: Discussionmentioning

confidence: 99%

Prevalence of dengue, Zika and chikungunya viruses in Aedes (Stegomyia) aegypti (Diptera: Culicidae) in a medium-sized city, Amazon, Brazil

Monteiro

Mourão

Ribeiro

et al. 2020

Rev. Inst. Med. trop. S. Paulo

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Aedes aegypti is associated with epidemic diseases in Brazil, such as urban yellow fever, dengue, and more recently, chikungunya and Zika viruses infections. More information about Ae. aegypti infestation is fundamental to virological surveillance in order to ensure the effectiveness of control measures in use. Thus, the present study aims to identify and compare infestation and infectivity of Ae. aegypti females in Macapa city, Amapa State (Amazon region), Brazil, between the epidemiological weeks 2017/02 and 2018/20. A total number of 303 Ae. aegypti females were collected at 21 fixed collection points, 171 at the 10 collection points in the Marabaixo neighborhood and 132 at the 11 collection points in the Central neighborhood. Among the collected samples, only two were positive for dengue virus, with a 2.08% (2/96 pools) infectivity rate for Marabaixo. The difference between the medians of Ae. aegypti females captured in Central and Marabaixo sites was not statistically significant. The findings indicate similar mosquito infestation levels between the neighborhoods, and a low-level of mosquito infectivity, although dengue virus was found only in Marabaixo. Virological surveillance of Ae. aegypti was important to identify sites of infection and determine possible routes of transmission to enable health surveillance teams to adopt preventive strategies where infected mosquitoes are present and act faster.

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Section: Discussionmentioning

confidence: 99%

Prevalence of dengue, Zika and chikungunya viruses in Aedes (Stegomyia) aegypti (Diptera: Culicidae) in a medium-sized city, Amazon, Brazil

Monteiro

Mourão

Ribeiro

et al. 2020

Rev. Inst. Med. trop. S. Paulo

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“…vertical transmission) in arthropods or by horizontal transmission via infected vertebrates. As horizontal transmission is dependent on the density of susceptible hosts and their intervals of patent infection, the transovarial and/or transstadial pathways may be more reliable routes to transmit intracellular parasites4041. Although transovarial and/or transstadial transmission has been documented in members of the Rickettsiaceae 15174243, this process is not thought to occur to a significant degree in those Anaplasma and Ehrlichia bacteria documented to date, which may in part be due to lack of the aldolase/adducing domain protein44.…”

Section: Discussionmentioning

confidence: 99%

Extensive genetic diversity of Rickettsiales bacteria in multiple mosquito species

Guo

Tian

Lin³

et al. 2016

Sci Rep

101

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Rickettsiales are important zoonotic pathogens, causing severe disease in humans globally. Although mosquitoes are an important vector for diverse pathogens, with the exception of members of the genus Wolbachia little is known about their role in the transmission of Rickettsiales. Herein, Rickettsiales were identified by PCR in five species of mosquitoes (Anopheles sinensis, Armigeres subalbatus, Aedes albopictus, Culex quinquefasciatus and Cu. tritaeniorhynchus) collected from three Chinese provinces during 2014–2015. Subsequent phylogenetic analyses of the rrs, groEL and gltA genes revealed the presence of Anaplasma, Ehrlichia, Candidatus Neoehrlichia, and Rickettsia bacteria in mosquitoes, comprising nine documented and five tentative species bacteria, as well as three symbionts/endosybionts. In addition, bacteria were identified in mosquito eggs, larvae, and pupae sampled from aquatic environments. Hence, these data suggest that Rickettsiales circulate widely in mosquitoes in nature. Also of note was that Ehrlichia and Rickettsia bacteria were detected in each life stage of laboratory cultured mosquitoes, suggesting that Rickettsiales may be maintained in mosquitoes through both transstadial and transovarial transmission. In sum, these data indicate that mosquitoes may have played an important role in the transmission and evolution of Rickettsiales in nature.

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“…aegypti larvae, where DENV IV was identified. Following genetic sequencing analysis of DENV, the authors showed similarity to the DENV IV genotype II strain from Manaus [42]. This is an important result because it demonstrates the origin and geographical distribution of the strains that circulate in the country.…”

Section: Discussionmentioning

confidence: 99%

Transovarial transmission of DENV in Aedes aegypti in the Amazon basin: a local model of xenomonitoring

et al. 2017

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BackgroundTransovarial transmission of dengue virus in Aedes spp. mosquitoes is considered an important mechanism for the maintenance of the virus in nature and may be implicated in the occurrence of outbreaks and epidemics of the disease. However, there are few studies involving transovarial transmission and viral vector monitoring as a surveillance tool and control strategy. The present study evaluated transovarial transmission of dengue virus in Aedes aegypti populations as a xenomonitoring strategy in municipalities of the Amazonas state.Results Aedes sp. eggs (13.164) were collected, with 30% viability of third- and fourth-instar larvae. Transovarial transmission of DENV was detected in all municipalities. The transovarial infection rate (TOR) in the municipalities was 46% of the DENV positive samples. The minimum infection rate (MIR) was 17.7 in the state, varying from 11.4 to 24.1 per 1,000 larvae tested in the respective municipalities. Four DENV serotypes were identified, with DENV I and IV being present in all municipalities investigated. The number of reported dengue fever cases varied during this period.ConclusionsOur results suggest that transovarial transmission may be an important mechanism for the maintenance and spreading of the disease in Amazonas municipalities. Using qRT-PCR, it was possible to identify the four DENV serotypes in larval samples. The methodology used in the present study proved suitable as a DENV xenomonitoring model in immature mosquitoes, contributing to the development of systems for early detection of viral circulation and predictive models for the occurrence of outbreaks and epidemics of this disease.Trial registration CAAE34025414200005015.

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Natural transovarial transmission of dengue virus 4 in Aedes aegypti from Cuiabá, State of Mato Grosso, Brazil

Cited by 33 publications

References 41 publications

Prevalence of dengue, Zika and chikungunya viruses in Aedes (Stegomyia) aegypti (Diptera: Culicidae) in a medium-sized city, Amazon, Brazil

Prevalence of dengue, Zika and chikungunya viruses in Aedes (Stegomyia) aegypti (Diptera: Culicidae) in a medium-sized city, Amazon, Brazil

Extensive genetic diversity of Rickettsiales bacteria in multiple mosquito species

Transovarial transmission of DENV in Aedes aegypti in the Amazon basin: a local model of xenomonitoring

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