Natural Variation in Elicitation of Defense-Signaling Associates to Field Resistance Against the Spot Blotch Disease in Bread Wheat (Triticum aestivum L.)

Sharma, Sandeep; Sahu, Ranabir; Navathe, Sudhir; Mishra, Vinod Kumar; Chand, Ramesh; Singh, Pawan K.; Joshi, Arun Kumar; Pandey, Sarvesh

doi:10.3389/fpls.2018.00636

Cited by 27 publications

(16 citation statements)

References 73 publications

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“…This might be due to the elevated endogenous phenolic content. Previous studies showed that logarithmic regression of inverse correlation showed that the disease progression/AUDPC of spot blotch disease in bread wheat, caused by the hemibiotrophic fungus Bipolaris sorokiniana, was strongly negatively correlated with the pathogen-induced content of phenolic acids, syringic acid, chlorogenic acid, 4-hydroxybenzoic acid, and caffeic acid [57,58]. Interestingly, our findings showed that the total soluble phenolic content increased markedly after the treatment with gallic acid and its derivatives.…”

Section: Discussionsupporting

confidence: 50%

The Antifungal Activity of Gallic Acid and Its Derivatives against Alternaria solani, the Causal Agent of Tomato Early Blight

et al. 2020

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Tomato (Solanum lycopersicum L.) is among the most important vegetable crops worldwide. Early blight disease, caused by Alternaria solani, is a destructive foliar disease of tomato and other Solanaceae species. Herein, we investigated the in vitro antifungal properties of gallic acid and two of its derivatives (syringic and pyrogallic acids) against A. solani during 2019 and 2020 seasons. The physiological and biochemical effects of these compounds on infected tomato plants were also investigated using the whole plant bioassay. The in vitro investigation showed that all tested compounds showed fungistatic action and inhibited the mycelial radial growth of A. solani in a dose-dependent manner. In two separate pot-experiments, those compounds efficiently suppressed the development of the disease symptoms and area under disease progress curve (AUDPC), without any phytotoxic effects on the treated tomato plants. Additionally, all tested compounds positively enhanced the biochemical traits of treated plants including the chlorophyll content, the total soluble phenolics, the total soluble flavonoids, and the enzymatic activities of catalase, peroxidase, and polyphenol oxidase during 2019 and 2020 seasons. Moreover, the treatment with gallic acid and its derivatives significantly increased all yield components of A. solani-infected tomato plants such as the total number of flowers and fruits, and the fruit yield for each tomato plant in both experiments. Considering the fungitoxicity of phenolic acids against A. solani with no phytotoxicity on treated tomato plants, we believe that gallic acid and its derivatives might be a sustainable eco-friendly control strategy to reduce the usage of chemical fungicides partially or entirely against A. solani particularly, and fungal diseases in general.

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Section: Discussionsupporting

confidence: 50%

The Antifungal Activity of Gallic Acid and Its Derivatives against Alternaria solani, the Causal Agent of Tomato Early Blight

et al. 2020

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“…We tested whether MBE02 might be effective in controlling the growth of a larger spectrum of fungal pathogens that affect different crop species. Therefore, growth inhibition assays were performed on 3 additional fungal pathogens: Bipolaris sorokiniana is a hemibiotrophic plant pathogen that causes spot blotch disease, and is a major threat to wheat and barley cultivation in south Asia 51 , 52 . Phytopthora drechsleri f. sp.…”

Section: Resultsmentioning

confidence: 99%

A halotolerant growth promoting rhizobacteria triggers induced systemic resistance in plants and defends against fungal infection

Sharma

Chen

Navathe

et al. 2019

Sci Rep

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A halotolerant rhizobacteria, Klebsiella species (referred to MBE02), was identified that had a growth stimulation effect on peanut. To gain mechanistic insights into how molecular components were reprogrammed during the interaction of MBE02 and peanut roots, we performed deep RNAsequencing. In total, 1260 genes were differentially expressed: 979 genes were up-regulated, whereas 281 were down-regulated by MBE02 treatment as compared to uninoculated controls. A large component of the differentially regulated genes were related to phytohormone signalling. This included activation of a significant proportion of genes involved in jasmonic acid, ethylene and pathogendefense signalling, which indicated a role of MBE02 in modulating plant immunity. In vivo and in vitro pathogenesis assays demonstrated that MBE02 treatment indeed provide fitness benefits to peanut against Aspergillus infection under controlled as well as field environment. Further, MBE02 directly reduced the growth of a wide range of fungal pathogens including Aspergillus. We also identified possible molecular components involved in rhizobacteria-mediated plant protection. Our results show the potential of MBE02 as a biocontrol agent in preventing infection against several fungal phytopathogens. Roots not only facilitate plants to uptake minerals and water from the soil but also serve as a major source of nutrients for microorganisms residing in the rhizosphere 1-3. Root exudates are rich in organic compounds, which attract microbial population to colonize the plant roots 4. Most of these microbes are neutral; however, many are pathogenic by nature and negatively affect the plant growth 1,5. On the other hand, there are few microorganisms such as, mycorrhizal fungi, rhizobium and plant growth promoting rhizobacteria (PGPR), which produce beneficiary changes and stimulate plant growth via direct or indirect mechanisms 3,4,6-8. One of these beneficiary changes include improved root architecture by increasing the number of root hairs and lateral root numbers. This may be correlated with the signalling of phytohormones, mainly auxin 2. This enlargement in root surface facilitates plants to uptake more water and nutrient, and in turn leads to improved growth. In addition, colonization of plant roots by few PGPR strains trigger an induced systemic resistance (ISR) response that functions systemically throughout the plant and is often effective against a broad spectrum of plant pathogens 5,9,10. In contrast to the costly defenses that often, upon activation, restricts the plant growth, ISR is associated with a defence priming phenomenon, which simultaneously stimulates plant growth and immunity 5,6,11,12. These beneficial microbes could be an ideal choice for developing them into biocontrol agents that enhances disease resistance in crop plants. Over the past few years, our understandings about molecular mechanisms of PGPR mediated growth promotion and disease resistance in plants has improved significantly as several genetic determinants that regulate these pr...

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“…In order to meet the future demands of food security, the challenge to wheat production is compounded by effectively controlled wide-spread occurrence of plant diseases (Sharma et al 2018).…”

Section: Discussionmentioning

confidence: 99%

Transcriptome analysis reveal the mechanism of susceptible wheat seedlings response to Puccinia striiformis f. sp.

Liu¹,

Chen²,

Zhou³

et al. 2020

Preprint

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Background Wheat (Triticum aestivum L.) is most widely cultivated and a major staple food crops in the world. Stripe rust caused by Puccinia striiformis f. sp. tritici (Pst), which significantly reduce yield and quality of wheat. Although some resistant genes have been successfully used in wheat breeding, large of the regulating networks and the underlying molecular mechanisms of Pst response remains unknown. Therefore, to identify differentially expressed genes (DEGs) and regulate network involved in Pst resistance, we sequenced 15 cDNA libraries constructed from wheat seedlings with CYR34 infection.Results In this study, a highly susceptible cv. Chuanyu12 (CY12) were used to study the transcriptome profiles after inoculated with Pst physiological race CYR34. A total of 13892, 10195, 12268 and 14044 DEGs were investigated at 24h, 48h, 72h and 7days Pst infection, respectively. Certain key genes and pathways responsible for Pst-CYR34 in CY12 were identified. The results revealed that Pst-CYR34 inhibited the DEGs related to energy metabolism, biosynthesis, carbon fixation, phenylalanine metabolism, and plant hormone signaling pathway after Pst inoculation at 24h, 48h, 72h and 7d. These down-regulated DEGs including light-harvesting chlorophyll protein complex in photosystem I and photosystem II; cytochrome b6/f/ complex, F-type ATPase and photosynthetic electron transport; ethylene, jasmonic acid (JA) and salicylic acid (SA); lignin and flavonoids biosynthesis in CY12. Quantitative Real-time PCR analysis verified the expression patterns of these DEGs.Conclusions Our results give insights into the foundation for further exploring the molecular mechanism regulating networks of Pst response and pave the way for durable resistant breeding in bread wheat.

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Natural Variation in Elicitation of Defense-Signaling Associates to Field Resistance Against the Spot Blotch Disease in Bread Wheat (Triticum aestivum L.)

Cited by 27 publications

References 73 publications

The Antifungal Activity of Gallic Acid and Its Derivatives against Alternaria solani, the Causal Agent of Tomato Early Blight

The Antifungal Activity of Gallic Acid and Its Derivatives against Alternaria solani, the Causal Agent of Tomato Early Blight

A halotolerant growth promoting rhizobacteria triggers induced systemic resistance in plants and defends against fungal infection

Transcriptome analysis reveal the mechanism of susceptible wheat seedlings response to Puccinia striiformis f. sp.

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