Natural Variation in OsLG3 Increases Drought Tolerance in Rice by Inducing ROS Scavenging

Abstract

Improving the performance of rice () under drought stress has the potential to significantly affect rice productivity. Here, we report that the ERF family transcription factor OsLG3 positively regulates drought tolerance in rice. In our previous work, we found that has a positive effect on rice grain length without affecting grain quality. In this study, we found that was more strongly expressed in upland rice than in lowland rice under drought stress conditions. By performing candidate gene association analysis, we found that natural variation in the promoter of is associated with tolerance to osmotic stress in germinating rice seeds. Overexpression of significantly improved the tolerance of rice plants to simulated drought, whereas suppression of resulted in greater susceptibility. Phylogenetic analysis indicated that the tolerant allele of may improve drought tolerance in cultivated rice. Introgression lines and complementation transgenic lines containing the elite allele of showed increased drought tolerance, demonstrating that natural variation in contributes to drought tolerance in rice. Further investigation suggested that plays a positive role in drought stress tolerance in rice by inducing reactive oxygen species scavenging. Collectively, our findings reveal that natural variation in contributes to rice drought tolerance and that the elite allele of is a promising genetic resource for the development of drought-tolerant rice varieties.

Knockout of the OsNAC006 Transcription Factor Causes Drought and Heat Sensitivity in Rice

“…To measure OsNAC006 expression levels following various abiotic stress and phytohormone treatments, 4-week-old WT seedlings grown in a light incubator at 28 • C in Hoagland solution under 16 h 3000 lux/8 h dark conditions were treated with cold (4 • C), heat (42 • C), PEG 6000 (20%, w/v), NaCl (200 mm), H 2 O 2 (1%), IAA (100 µm), ABA (100 µm) and GA 3 (100 µm) [45]. Leaf tissue was harvested after stress treatment and subjected to RT-qPCR analysis.…”

Natural alleles of GLA for grain length and awn development were differently domesticated in rice subspecies japonica and indica

“…Genomic DNA fragments of GLA from different accessions were amplified using the V1, V2, V3 and V4 primers (Data S1 and S2), sequenced and analysed by DNASTAR software. Variable sites, including 33 SNPs (single nucleotide polymorphisms) and 13 InDels (insertion/deletion polymorphisms), were used for GLA-based association analysis Wang et al, 2016;Xiong et al, 2018). Significant sites based on the -log 10 (P) values were used for haplotype analyses (Data S2).…”

Regulatory changes in TaSNAC8‐6A are associated with drought tolerance in wheat seedlings

“…SNPs) or genetic markers within, or tightly linked to genes contributing to complex traits (Huang et al, 2011;Liu et al, 2017;Wang and Qin, 2017;Xiao et al, 2017). This approach has previously been successfully applied to detect allelic diversity of genes controlling crop drought tolerance, especially in rice and maize (Liu et al, 2013;Mao et al, 2015;Singh et al, 2015;Songyikhangsuthor et al, 2014;Wang et al, 2016;Xiang et al, 2017;Xiong et al, 2018).…”