Natural variation in iron use efficiency and mineral remobilization in cucumber (Cucumis sativus)

Waters, Brian M.; Troupe, Grace

doi:10.1007/s11104-011-0988-3

Cited by 27 publications

(20 citation statements)

References 78 publications

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“…As low Fe supply caused plants to accumulate additional Cu in leaves (Welch et al ., ; Chaignon et al ., ; Waters & Troupe, ; Waters et al ., ), we hypothesized that under low Cu supply there would be a higher demand for Fe, which should lead to increased Fe uptake and rescue the fefe phenotype. This hypothesis was correct, as fefe plants grown without Cu (+Fe, −Cu) recovered within 9 d and had a green first leaf phenotype, while plants grown on complete solution (+Fe, +Cu) had the typical yellow first leaf (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…In previous work we showed that cucumber cotyledons grow (e.g. increase in DW) and accumulate Fe and certain other minerals over the first few days after germination (Waters & Troupe, 2012). To test whether fefe accumulated Fe or utilized Fe stored in cotyledons, we measured DW and Fe content of cotyledons in the WT and the fefe mutant ( Fig.…”

Section: Physiological Characterization the Fefe Mutantmentioning

confidence: 99%

“…Fe homeostasis interacts with Zn tolerance (Pineau et al ., ), and Cu deficiency interacts with phosphate signaling (Perea‐García et al ., ) and cadmium tolerance (Gayomba et al ., ). Copper concentration was higher in Fe‐deficient leaves (Welch et al ., ; Chaignon et al ., ; Waters & Troupe, ; Waters et al ., ). Several Cu‐responsive genes and microRNAs had altered abundance under Fe deficiency in Arabidopsis thaliana (Stein & Waters, ; Waters et al ., ).…”

Section: Introductionmentioning

confidence: 99%

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Transcriptomic and physiological characterization of the fefe mutant of melon (Cucumis melo) reveals new aspects of iron–copper crosstalk

2014

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Summary Iron (Fe) and copper (Cu) homeostasis are tightly linked across biology. In previous work, Fe deficiency interacted with Cu regulated genes and stimulated Cu accumulation. The C940-fe (fefe) Fe uptake mutant of melon (Cucumis melo) was characterized, and the fefe mutant was used to test whether Cu deficiency could stimulate Fe uptake. Wild type and fefe mutant transcriptomes were determined by RNA-seq under Fe and Cu deficiency. FeFe regulated genes included core Fe uptake, metal homeostasis, and transcription factor genes. Numerous genes were regulated by both Fe and Cu. The fefe mutant was rescued by high Fe or by Cu deficiency, which stimulated ferric-chelate reductase activity, FRO2 expression, and Fe accumulation. Accumulation of Fe in Cu deficient plants was independent of the normal Fe uptake system. One of the four FRO genes in the melon and cucumber (Cucumis sativus) genomes was Fe regulated, and one was Cu regulated. Simultaneous Fe and Cu deficiency synergistically upregulated Fe uptake gene expression. Overlap in Fe and Cu deficiency transcriptomes highlights the importance of Fe– Cu crosstalk in metal homeostasis. The fefe gene is not orthologous to FIT, thus identification of this gene will provide clues to help understand regulation of Fe uptake in plants.

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Section: Resultsmentioning

confidence: 99%

Section: Physiological Characterization the Fefe Mutantmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Transcriptomic and physiological characterization of the fefe mutant of melon (Cucumis melo) reveals new aspects of iron–copper crosstalk

2014

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“…10,11 Cu concentrations in Arabidopsis and other species were higher in Fe-deficient leaves. [11][12][13][14] Together, this suggested that accumulation of Cu under Fe deficiency allows replacement of FeSOD proteins with CuSOD proteins. A physiological role for this switch from FeSODs to CuSODs was demonstrated, because inability to make this switch resulted in decreased ability to counteract oxidative stress.…”

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confidence: 93%

Optimal copper supply is required for normal plant iron deficiency responses

Waters

Armbrust

2013

Plant Signaling & Behavior

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“…In addition, high [HCO3 -] and high pH in calcareous soils are considered the main factors affecting the sensitivity of some Lupinus species to calcareous soils (Coulombe et al 1984;Mengel et al 1984;Romera et al 1992;Tang and Thomson 1996;Waters and Troupe 2012).…”

Section: Introductionmentioning

confidence: 99%

Is pH the key reason why some Lupinus species are sensitive to calcareous soil?

2018

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Aims: Previous studies have shown that pH, rather than calcium (Ca), is the main reason why some Lupinus species are sensitive to nutrient solutions mimicking calcareous soils; however, a hydroponic system is quite different from soil systems, and plants may respond differently to these two growing conditions. Thus, studies with Lupinus species grown in calcareous soils are needed. Methods: Two calcicole and two calcifuge species were grown in river sand with different Ca forms and amounts, pH levels, and [bicarbonate (HCO3-)] (which is produced by calcium carbonate (CaCO3)). Leaf symptoms, leaf area, gas exchange, biomass, and root morphology were recorded; whole leaf and root nutrient concentrations were analysed. Results: We observed leaf chlorosis of the youngest leaves under high pH (adjusted by KOH) and high pH + high Ca (representing high [HCO3-], high pH and high Ca) treatments for all Lupinus species. However, after two weeks, leaf chlorosis of all Lupinus species under high pH started to disappear, with calcicole species fully, and calcifuge species only partly recovering. Leaf chlorosis symptoms of calcicole species under high pH + high Ca partly disappeared as well, while those of calcifuge species did not disappear at all. Conclusions: High pH (resulting from either KOH or HCO3-) inhibited root growth, and subsequently uptake of some nutrients and shoot growth of Lupinus species. However, the strong buffering capacity of HCO3is the key factor determining if Lupinus species can survive in calcareous soils. Among all studied Lupinus species, L. pilosus was the most tolerant to high [HCO3-] and/or high pH, followed by L. cosentinii and L. angustifolius, while L. hispanicus was the most sensitive.

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Natural variation in iron use efficiency and mineral remobilization in cucumber (Cucumis sativus)

Cited by 27 publications

References 78 publications

Transcriptomic and physiological characterization of the fefe mutant of melon (Cucumis melo) reveals new aspects of iron–copper crosstalk

Transcriptomic and physiological characterization of the fefe mutant of melon (Cucumis melo) reveals new aspects of iron–copper crosstalk

Optimal copper supply is required for normal plant iron deficiency responses

Is pH the key reason why some Lupinus species are sensitive to calcareous soil?

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