Natural zeranol (.alpha.-zearalanol) in the urine of pasture-fed animals

Erasmuson, Anton Francis; Scahill, B.G.; West, D.M.

doi:10.1021/jf00048a014

Cited by 74 publications

(62 citation statements)

References 6 publications

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“…The authors suggest that the formation of α-ZAL may occur primarily via reduction of α-ZOL, probably in the reductive environment of the rumen. The results are in agreement with earlier investigations which implied that α-ZAL can occur in domestic animals without deliberate α-ZAL treatment (Erasmuson et al, 1994;Kennedy et al, 1995;Miles et al, 1996; Figure 2). Moreover, Kennedy et al (1998) found significantly higher α-ZOL (12-fold) and β-ZOL (9-fold) concentrations in the α-ZAL positive samples than in the α-ZAL negative samples with an α-ZAL: α-ZOL ratio of at least 1:5.…”

Section: Deoxynivalenolsupporting

confidence: 93%

Relevance of the <i>Fusarium</i> toxins deoxynivalenol and zearalenone in ruminant nutrition. A review

Seeling¹,

Dänicke²

2005

J. Anim. Feed Sci.

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Deoxynivalenol (DON) and zearalenone (ZON) produced by Fusarium species are agriculturally important mycotoxins of relevance to livestock health. While ZON is known to cause oestrogenic syndromes in animals, a moderate ingestion of DON is associated with reduced performance and immune function. Among farm animals, ruminants appear to have a higher tolerance towards the effects of DON and ZON. As a consequence, feed producers may allocate cereals and roughages which appear contaminated with Fusarium toxins to ruminants. In combination with enhanced toxin concentrations during so-called Fusarium years, the possible effects in ruminants cannot be overlooked. However, only limited and inconsistent literature data are available about the effects of DON and ZON on ruminants. On the basis of the literature, the kinetics, biotransformation and carry over, as well as the effects of DON and ZON on ruminants, are reviewed. Furthermore, dosage and duration of toxin exposure as well as genetic and physiological factors of ruminants which could influence the variability of the toxin effects are considered and discussed. It is concluded that additional research is needed to study the effects of DON and ZON on ruminants, especially in lactating dairy cows.

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Section: Deoxynivalenolsupporting

confidence: 93%

Relevance of the <i>Fusarium</i> toxins deoxynivalenol and zearalenone in ruminant nutrition. A review

Seeling¹,

Dänicke²

2005

J. Anim. Feed Sci.

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“…Erasmuson et al [17] demonstrated that α-Zol and β-Zol can be reduced further, resulting in significant amounts of α-zearalanol or zeranol (α-Zal) and β-zearalanol or taleranol (β-Zal) in mammal urine.…”

Section: Introductionmentioning

confidence: 99%

Comparative study of toxic effects of zearalenone and its two major metabolites α‐zearalenol and β‐zearalenol on cultured human Caco‐2 cells

Abid‐Essefi

Bouaziz

Golli-Bennour

et al. 2009

J Biochem & Molecular Tox

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Zearalenone (ZEN) is a fusarotoxin converted predominantly into alpha-zearalenol (alpha-Zol) and beta-zearalenol (beta-Zol) by hepatic hydroxysteroid dehydrogenases. The feeding of naturally contaminated grains with ZEN was associated with hyperestrogenic and adverse effects on humans and animals. There is a lack of information on the attribution of the toxic effects of these toxins. One wonders if these effects are due to the parent molecule (ZEN) or to its major metabolites (alpha-Zol and beta-Zol). Using human Caco-2 cells, we looked for the molecular mechanisms of toxicity of ZEN, alpha-Zol, and beta-Zol. Toxicity effects were studied by MTT viability assay and oxidative stress induction by measuring malondialdehyde (MDA) generation. To check whether the oxidative stress induction was associated to DNA lesions, we looked for DNA fragmentation by means of the Comet and the diphenylamine assays. To specify cell death pathway, we investigated caspase-3 activation, confirmed by poly(ADP-ribose) polymerase cleavage and by Bcl-2 depletion. Our results clearly demonstrated that ZEN as well as its two metabolites presented variable toxic effects. They induced cell death and an increase in MDA generation. These effects were associated to DNA fragmentation as well as caspase-3 activation. The observed toxic effects seem to be relieved by the metabolism of ZEN into alpha-Zol and beta-Zol.

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“…The usefulness of the method developed was demonstrated via several contaminated cow and pig urine samples. the mycotoxins zearalenone (ZON) and e-zearalenol (7-ZOL) that can be carried over from contaminated feedstuffto animals [2][3][4]. ZON, e-ZOL and its epimer/%zearalenol (fl-ZOL) are produced by several Fusarium species that colonize grains [5].…”

mentioning

confidence: 99%

Determination of zeranol, taleranol, zearalenone, α- and β-zearalenol in urine and tissue by high-performance liquid chromatography-tandem mass spectrometry

2000

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Key WordsColumn liquid chromatography Tandem mass spectrometry Zeranol, zearalenone and zearalenol SummaryAn LC-MS/MS method has been developed for the sensitive determination of the anabolic compound zeranol, its epimer taleranol and the mycotoxins zearalenone, azearalenol and fl-zearalenol in animal urine and meat samples. Sample preparation included extraction of meat samples and enzymatic digest of urine samples followed by solid phase extraction with RP-18 columns for sample clean-up. Mass spectrometric determination was carried out with an atmospheric pressure chemical ionisation interface (APCI) in the multi-reaction monitoring mode (MRM). Using the negative ion mode, a limit of detection between 0.1 and 0.5 ppb and a limit of determination between 0.5 and 1 ppb could be achieved. With zearalanone (ZAN) as internal standard, a linear range between 0.5 (1.0) and 100ppb in urine samples (cow, pig) and between 1 and 100 ppb in meat samples (cow, calf, pig) could be established. Depending on the biological matrix and analyte, standard deviations were below 8.5 %, with average recovery rates around 86 % to 102 % in spiked samples. The usefulness of the method developed was demonstrated via several contaminated cow and pig urine samples. the mycotoxins zearalenone (ZON) and e-zearalenol (7-ZOL) that can be carried over from contaminated feedstuffto animals [2][3][4]. ZON, e-ZOL and its epimer/%zearalenol (fl-ZOL) are produced by several Fusarium species that colonize grains [5]. High amounts can be found on maize, wheat, barley and oats. All three compounds exhibit distinct HC

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Natural zeranol (.alpha.-zearalanol) in the urine of pasture-fed animals

Cited by 74 publications

References 6 publications

Relevance of the <i>Fusarium</i> toxins deoxynivalenol and zearalenone in ruminant nutrition. A review

Relevance of the <i>Fusarium</i> toxins deoxynivalenol and zearalenone in ruminant nutrition. A review

Comparative study of toxic effects of zearalenone and its two major metabolites α‐zearalenol and β‐zearalenol on cultured human Caco‐2 cells

Determination of zeranol, taleranol, zearalenone, α- and β-zearalenol in urine and tissue by high-performance liquid chromatography-tandem mass spectrometry

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