Naturally derived biomaterials for addressing inflammation in tissue regeneration

Abstract

Tissue regeneration strategies have traditionally relied on designing biomaterials that closely mimic features of the native extracellular matrix (ECM) as a means to potentially promote site-specific cellular behaviors. However, inflammation, while a necessary component of wound healing, can alter processes associated with successful tissue regeneration following an initial injury. These processes can be further magnified by the implantation of a biomaterial within the wound site. In addition to designing biomaterials to satisfy biocompatibility concerns as well as to replicate elements of the composition, structure, and mechanics of native tissue, we propose that ECM analogs should also include features that modulate the inflammatory response. Indeed, strategies that enhance, reduce, or even change the temporal phenotype of inflammatory processes have unique potential as future pro-regenerative analogs. Here, we review derivatives of three natural materials with intrinsic anti-inflammatory properties and discuss their potential to address the challenges of inflammation in tissue engineering and chronic wounds.

A novel supercritical CO 2 -based decellularization method for maintaining scaffold hydration and mechanical properties

“…In this chamber scCO 2 and the water additive were stirred vigorously until reaching thermodynamic equilibrium (about 15 min). At this point, flow was introduced into the 10 mL treatment chamber (6), which contained the matrix material. Constant flow was maintained by the syringe pump at 1 mL/min for the desired treatment time (usually about 1 h).…”

“…This is a particular strength of naturally-derived biomaterials, which have recently been shown to promote constructive remodeling during tissue growth [4,5]. Decellularized ECM has also been shown to elicit an anti-inflammatory immune response, which may be related to a reduced risk of rejection [6,7]. Decellularization is accomplished using a variety of techniques, including physical [8], chemical [9], and enzymatic treatment methods [10].…”

Tracheal Replacement Using an In-Body Tissue-Engineered Collagenous Tube “BIOTUBE” with a Biodegradable Stent in a Beagle Model: A Preliminary Report on a New Technique

“…Following the proliferation phase, the tissue remodeling phase starts a few months after the injury. 21 This study showed that about 2 months after the implantation, the lumen became stable. This point might be considered as the transitional point from the proliferation phase to the remodeling phase.…”

Decellularized Aortic Scaffold Alleviates H2O2‐Induced Inflammation and Apoptosis in CD34+ Progenitor Cells While Driving Neovasculogenesis

“…Some inflammatory cytokines, including IL-1, IL-4, IL-6, IL-8, IL-18, and TGF-β1, are involved in the development of ischemic heart disease, myocardial infarction, and heart failure, thus, serving as potential targets for the development of some anti-ischemic therapies [24]. It was reported that the decellularized matrix has immunomodulatory properties and the ability to influence the host response in vivo [25]. us, we performed a protein chip assay to detect the expression of proinflammatory cytokines and anti-inflammatory cytokines when a cell or cell-scaffold construct was exposed to H 2 O 2 .…”