Naturally occurring differential drug sensitivities of cultured plant protoplasts

Cocking, E. C.; Power, J. B.; Evans, P. K.; Safwat, F.; Frearson, E.M.; Hayward, C.; Berry, S.F.; George, D.

doi:10.1016/0304-4211(74)90006-6

Cited by 33 publications

(2 citation statements)

References 11 publications

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“…Accordingly, the optimal nylon mesh size and centrifugation speeds were 300 and 50 G, respectively. Many other methods of purification have also been reported, such as flotation on dense sucrose or Ficoll solutions (Cocking et al 1974). However, the protoplasts purified via such methods are more viscous than those purified by filtration and centrifugation and cannot be used in additional experiments except in protoplast regeneration.…”

Section: Effects Of the Nylon Mesh Size And Centrifugation Speed On Protoplast Purificationmentioning

confidence: 99%

Isolation and Purification of Mesophyll Protoplasts from <i>Ginkgo biloba</i> L.

et al. 2020

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Techniques for protoplast isolation from various plants are widely applied. The purpose of this work is to develop an efficient system for isolating and purifying mesophyll protoplasts from a gymnosperm, Ginkgo biloba L. We tried to optimize main factors influencing the isolation of mesophyll protoplasts from G. biloba, including the enzyme type and concentration, incubation time, enzyme solution pH, osmotic pressure, nylon mesh size, and centrifugation speed. The suitable enzyme digestion system consisted of digestion by 2% (w/v) cellulase R-10, 0.2% (w/v) pectolyase Y-23 and 1.5% (w/v) macerozyme R-10 for 5 h in the dark at 25 C. After filtration through a 300-mesh nylon membrane and centrifugation at 50 G, the yield and viability of the obtained protoplasts reached approximately 5.39 10 6 protoplasts g 1 fresh weight (FW) and 80.23%, respectively, Then, the yield and viability of protoplasts isolated via the same method in two different years were compared under the optimal conditions. It proved that the protocol was repeatable and effective.

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Section: Effects Of the Nylon Mesh Size And Centrifugation Speed On Protoplast Purificationmentioning

confidence: 99%

Isolation and Purification of Mesophyll Protoplasts from <i>Ginkgo biloba</i> L.

et al. 2020

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“…Various workers have experimented with naturally-occurring differential drug sensitivity as a basis for hybrid selection (Cocking et al, 1974;Maliga et al, 1977) and it is likely that more use of this will be made in the future.…”

Section: Protoplast Technology (I) Protoplast Isolationmentioning

confidence: 99%

The uses of isolated protoplasts in plant genetics

Cove

1979

Heredity

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SUMMARYProtoplasts may be isolated from a wide range of fungal and green plant species. In many of these, protoplasts can be induced to regenerate, and to give either plants directly, or cell cultures, which may in turn subsequently give rise to plants. Fusion of protoplasts can be induced by a variety of agents, and it may be possible to select hybrid cell lines or plants following treatment of mixtures of protoplasts. Hybrid selection procedures include direct visual selection using fusion partners which ale morphologicaily distinct, and various procedures which involve the use of complementing mutant strains.Somatic hybridisation following protoplast fusion has led to the selection of novel hybrids, not obtainable by sexual hybridisation, and has made possible the genetic analysis of sexually sterile strains. It also provides a unique method of studying cytoplasmic inheritance in higher plants, and allows the construction of strains having new combinations of nuclear and cytoplasmic genomes.Isolated protoplasts are likely to be excellent material for mutagenic treatment and because they take up a wide range of material, they are also valuable in a variety of genetic techniques, aimed at the transfer of specific genetic information into plant species. Protoplasts have been utilised in the experiments leading to transformation in Saccharomyces cerevisiae.

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Techniques of Somatic Cell Hybridization by Fusion of Protoplasts

Binding¹,

Nehls²

1982

Techniques in Somatic Cell Genetics

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Naturally occurring differential drug sensitivities of cultured plant protoplasts

Cited by 33 publications

References 11 publications

Isolation and Purification of Mesophyll Protoplasts from <i>Ginkgo biloba</i> L.

Isolation and Purification of Mesophyll Protoplasts from <i>Ginkgo biloba</i> L.

The uses of isolated protoplasts in plant genetics

Techniques of Somatic Cell Hybridization by Fusion of Protoplasts

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