Naturally Occurring SLE Anti‐DNA Antibodies Recognize Unique Conformation on DNA‐Lysine Photoadduct

Abstract

Native calf thymus DNA has been covalently modified with lysine under

Polyamine induced Z‐conformation of native calf thymus DNA

“…Immunoglobulins were fractionated by 40% saturated ammonium sulphate precipitation of the antiserum. The crude immunoglobulins after extensive dialysis in PBS were subjected to DEAE Sephacel chromatography followed by affinity purification through a Sepharose 4B column linked with immunogen [18,19]. The homogeneity of the isolated anti-ZDNA IgG was confirmed by the observation of a single band in a polyacrylamide gel corresponding to a molecular mass of 150,000.…”

Binding of SLE Autoantibodies to Native poly(I), ROS-poly(I) and Native DNA: A Comparative Study

“…Systemic lupus erythematosus (SLE) is a prototype autoimmune and multifactorial disease characterized by the presence of autoantibodies to a diverse array of nuclear antigens including DNA, RNA and histones [1][2][3]. These naturally occurring autoantibodies are heterogeneous, exhibiting a wide heterogeneity in the recognition of nucleosides and nucleotides [4].…”

Cross‐Reactions of Human Lupus Autoantibodies with 8‐Methoxypsoralen Photomodified DNA Fragments

“…It has been postulated that immune response against host antigens could result from genetic predisposition, exaggerated random B-cell activity, cross-reactivity between foreign and host antigens or modification of host antigen as a consequence of infection, inflammation, drug administration, hydroxyl/free radicals, environment, etc. (2,6,7,9). Sera derived from SLE individuals have been shown to possess anti-DNA antibodies of diverse antigenic specificity (1,4,16).…”

“…The origin of autoantibodies in systemic lupus erythematosus is unknown, and the mechanism that accounts for the diversity of serological abnormalities in this disease is poorly understood (2). Patients with SLE manifest a variety of immune abnormalities;…”