Enthusiasm for and investments in nature-based solutions (NBS) as sustainable strategies for climate adaptation and infrastructure development is building among governments, the scientific community, and engineering practitioners. This is particularly true for water security and water-related risks. In a freshwater context, NBS may provide much-needed “win-wins” for society and the environment that could benefit imperiled freshwater biodiversity. Such conservation benefits are urgently needed given the ongoing freshwater biodiversity crisis, with declines in freshwater species and their habitats occurring at more than twice the rate of marine or terrestrial systems. However, for NBS to make meaningful contributions to safeguarding freshwater biodiversity, clear links must be established between NBS applications and priorities for conservation. In this paper, we link common water-related NBS to six priority actions for freshwater life established by the conservation science n community, and highlight priority research and knowledge that will be necessary to bring NBS to bear on the freshwater biodiversity crisis. In particular, we illustrate how NBS can play a direct role in restoring degraded aquatic and floodplain ecosystems, enhancing in-stream water quality, and improving hydrological connectivity among freshwater ecosystems. System-level monitoring is needed to ensure that freshwater NBS deliver on their promised benefits for ecosystems and species.