Nature’s Own Pharmacy: Mushroom-Based Chemical Scaffolds and Their Therapeutic Implications

Hassan, Mubashir; Shahzadi, Saba; Ransom, Richard F.; Kloczkowski, Andrzej

doi:10.3390/ijms242115596

Cited by 5 publications

(1 citation statement)

References 143 publications

(161 reference statements)

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“…Considering the high nutritional and nutraceutical properties and their peculiar structural compositions, mushrooms could be a promising scaffold for growing disease-free alternative animal protein in the laboratory. Mushrooms are a significant source of essential nutrients, including carbohydrates, dietary fibers, vitamins (B1, B2, B12, C, D, and E), minerals, and proteins [ 24 ]. Also, mushrooms exhibit antibacterial, immune system enhancer, and cholesterol-lowering properties and are thus useful for promoting human health [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

Derivation and Characterization of Novel Cytocompatible Decellularized Tissue Scaffold for Myoblast Growth and Differentiation

Singh,

Kumar

et al. 2023

Cells

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The selection of an appropriate scaffold is imperative for the successful development of alternative animal protein in the form of cultured meat or lab-grown meat. Decellularized tissues have been suggested as a potential scaffold for cultured meat production owing to their capacity to support an optimal environment and niche conducive to cell proliferation and growth. This approach facilitates the systematic development of 3D tissues in the laboratory. Decellularized scaffold biomaterials have characteristics of high biocompatibility, biodegradation, and various bioactivities, which could potentially address the limitations associated with synthetic bio-scaffold materials. The present study involved the derivation and characterization of a decellularized scaffold from mushroom tissue following subsequent assessment of the scaffold’s capacity to support myogenic differentiation. Mushroom sections were soaked in nuclease and detergent solution for 4 days. Furthermore, decellularization was confirmed by histology and DAPI staining, which showed the removal of cellular components and nuclei. Myoblast cells were seeded onto decellularized tissue, which exhibited excellent cytocompatibility and promoted myogenic growth and differentiation. The study’s findings can serve as a foreground for the generation of an edible and natural scaffold for producing a safe and disease-free source of alternative animal protein, potentially reducing the burden on the health sector caused by conventional animal protein production and consumption.

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Section: Introductionmentioning

confidence: 99%