This study explores the Potaxie, Fifes, and Tilinx subcultures on TikTok, examining their origins, characteristics, and cultural significance. Originating from a viral video in 2020, the Potaxie subculture emerged within the Spanish-speaking LGBTQ+ community and evolved to symbolise inclusivity and gender equality. Potaxies use vibrant aesthetics influenced by Japanese and Korean pop culture to express their identities and resistance. In contrast, Fifes, associated with cisgender heterosexual men, embody traditional patriarchal values, often sexist and homophobic, creating a narrative of resistance between the groups. The Tilinx, symbolic descendants of the Potaxies, are inspired by ballroom culture and drag houses, with “Potaxie mothers” continuing the fight for inclusion and diversity. Using a mixed-methods approach, including quantitative analysis through the TikTok API and qualitative content analysis via MAXQDA and Python, this study provides a comprehensive understanding of the subculture that accumulates over 2.3 billion interactions. The findings highlight how TikTok serves as a platform for identity construction, cultural resistance, and the redefinition of social norms. Additionally, the study examines how digital platforms mediate intersectional experiences, favouring certain types of content through algorithms, and how participants navigate these opportunities and constraints to express their intersecting identities. The implications for communication strategies, youth policies, educational plans, and research on the commercialization of these subcultures are profound, offering insights into the transformative potential of social media in shaping contemporary cultural and social narratives.