Nax channel involved in CNS sodium-level sensing

Hiyama, Takeshi Y.; Watanabe, Eiji; Ono, Kentaro; Inenaga, Kiyotoshi; Tamkun, Michael M.; Yoshida, Shigeru; Noda, Masaharu

doi:10.1038/nn0602-856

Cited by 161 publications

(139 citation statements)

References 11 publications

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“…I.2A; Goldin et al, 2000). elevations in body fluids within the physiological range (Hiyama et al, 2002;Noda and Hiyama, 2005;Noda, 2006;Watanabe et al, 2006;Noda and Sakuta, 2013;Noda and Hiyama, 2015a;Noda and Hiyama, 2015b). The sensitivity of Na x to [Na + ] was revealed to be upregulated by endothelin-3 through activation of endothelin receptor B (Hiyama et al, 2013).…”

Section: Na X Channel Modulates Salt Appetite According To the Body-fmentioning

confidence: 99%

Distinct neural mechanisms for the control of thirst and salt appetite in the subfornical organ

et al. 2016

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Section: Na X Channel Modulates Salt Appetite According To the Body-fmentioning

confidence: 99%

Distinct neural mechanisms for the control of thirst and salt appetite in the subfornical organ

et al. 2016

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“…After blocking with 4% non-fat dry milk and 0.1% Tween 20 in PBS, the cells were incubated with rabbit anti-Na x [2] and mouse anti-SAP97 (Cat. No.…”

Section: Immunofluorescence Cell Stainingmentioning

confidence: 99%

“…Intracellular Na + imaging with sodium-binding benzofuran isophthalate acetoxymethyl ester (SBFI/AM; Molecular Probes) was performed as described [2].…”

Section: Intracellular Na + Imagingmentioning

confidence: 99%

SAP97 promotes the stability of Na_x channels at the plasma membrane

et al. 2012

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a b s t r a c t Na x is a sodium-level sensor for body fluids expressed in the circumventricular organs in the brain. Na x has a putative PSD-95/Disc-large/ZO-1 (PDZ)-binding motif at the carboxyl (C)-terminus. Here we found that several PDZ proteins bind to Na x by PDZ-array overlay assay. Among them, synapse-associated protein 97 (SAP97/DLG1) was coexpressed with Na x in the subfornical organ. In C6 glioblastoma cells, destruction of the PDZ-binding motif of Na x or depletion of SAP97 resulted in a decrease in cell-surface Na x , which was attenuated with inhibitors of endocytosis. These results indicate that SAP97 contributes to the stabilization of Na x channels at the plasma membrane. Structured summary of protein interactions:Nax physically interacts with SAP97 by anti tag coimmunoprecipitation (View interaction) CNRasGEF binds to Nax by protein array (View interaction) Nax and SAP97 colocalize by fluorescence microscopy (View interaction) GIPC1 binds to Nax by protein array (View interaction) ZO-1 binds to Nax by protein array (View interaction) SAP97 binds to Nax by protein array (View interaction) Densin-180 binds to Nax by protein array (View interaction) Beta-1-syntrophin binds to Nax by protein array (View interaction) ERBIN binds to Nax by protein array (View interaction) Nax physically interacts with SAP97 by pull down (View interaction) Lnx1 binds to Nax by protein array (View interaction) nNOS binds to Nax by protein array (View interaction)

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“…The epithelial sodium channel (ENaC ; Table 1), presumed to be the main mechanism of salt taste perception in taste buds, functions as a mechanism for sodium gating in kidney, lung and colon (Hiyama et al 2002). The candidate sour taste receptor Pkd2l1 (Table 1), although expressed in taste buds, is involved in triggering action potentials in response to decreases in extracellular pH in neurons present in the spinal cord (Huang et al 2006).…”

Section: Taste Receptors In Non-taste Tissues and The Diffused Chemosmentioning

confidence: 99%

Unfolding the codes of short-term feed appetence in farm and companion animals. A comparative oronasal nutrient sensing biology review

Roura¹,

Humphrey²,

Tedó³

et al. 2008

Can. J. Anim. Sci.

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Roura, E., Humphrey, B., Tedo´, G. and Ipharraguerre, I. 2008. Unfolding the codes of short-term feed appetence in farm and companion animals. A comparative oronasal nutrient sensing biology review. Can. J. Anim. Sci. 88: 535Á558. The evolution of the chemical senses has resulted in a sensory apparatus for high taste and smell acuity in mammals and birds to ensure self-nourishment. Such peripheral chemosensory systems function as a code to unfold the nutritional value of feedstuffs. Food ingestion simultaneously evokes odor, taste and thermo-mechanical (somatosensing) sensations. Olfaction represents the capacity to identify feed volatiles that are predominantly derived from essential nutrients in plants.Comparative biology of olfaction shows that primates and chickens have a smaller olfactory epithelium and fewer olfactory receptor (OR) genes than non-primate mammals studied to date including farm and companion animals, such as the pig, the cow, the dog, the cat and the horse. A significant proportion of the total OR genes in mammals and birds have lost their functionality (pseudogenes) in a process that seems to reflect a decrease in the animal's reliance on the sense of smell, particularly in humans and cows. The taste system allows animals to recognize a diverse repertoire of nutrient (sugars, amino acids, salts, acids and fats) or toxic related chemical entities that provide valuable information about the quality of food. Taste senses non-volatile molecules in the oral cavity through taste receptors (TR). The TR are expressed in the sensory cells forming the taste buds of the tongue's papillae. Taste cells are linked to a network of solitary chemosensory cells diffused through many non-taste tissues involved in metabolic homeostasis. The number of functional taste receptor genes (TASR) in humans is equivalent to that in other mammals and superior to that in chickens. The TASR family 1 (TAS1R coding for umami and sweet TR) is conserved, in number and type, across the species evaluated, with the exception of the sweet receptor in chicken and feline species. The TASR family 2 (TAS2R coding for bitter TR) shows a strong adaptive capacity to dietary sources and digestive physiology across vertebrates. Pseudogenization (loss of gene functionality) in the TAS2R family seems to be a frequent strategy. The implications of oronasal nutrient sensing related to comparative animal feeding strategies and behaviors such as neophobia, feed refusal and hedonic preferences are discussed. Feed palatability and appetence might be one of the main driving forces in short-term feed consumption. Finally, practical applications relevant to animal production are outlined.Key words: Nutrient sensing, taste, olfaction, somatosensing, feed intake, farm, companion animals Roura, E., Humphrey, B., Tedo´, G. et Ipharraguerre, I. 2008. Le code de l'appe´tence a`court terme chez les animaux domestiques et de compagnie. Biologie comparative de la de´tection bucco-nasale des e´le´ments nutritifs. Can. J. Anim. Sci. 88: 535Á558. En e´voluant, ...

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Nax channel involved in CNS sodium-level sensing

Cited by 161 publications

References 11 publications

Distinct neural mechanisms for the control of thirst and salt appetite in the subfornical organ

Distinct neural mechanisms for the control of thirst and salt appetite in the subfornical organ

SAP97 promotes the stability of Na_x channels at the plasma membrane

Unfolding the codes of short-term feed appetence in farm and companion animals. A comparative oronasal nutrient sensing biology review

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Nax channel involved in CNS sodium-level sensing

Cited by 161 publications

References 11 publications

Distinct neural mechanisms for the control of thirst and salt appetite in the subfornical organ

Distinct neural mechanisms for the control of thirst and salt appetite in the subfornical organ

SAP97 promotes the stability of Nax channels at the plasma membrane

Unfolding the codes of short-term feed appetence in farm and companion animals. A comparative oronasal nutrient sensing biology review

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SAP97 promotes the stability of Na_x channels at the plasma membrane