ncRNA-mediated low expression of P2RY14 correlates with poor prognosis and tumor immune infiltration in ovarian carcinoma

Abstract

Background: Ovarian cancer (OV) has been puzzling clinicians because of its poor prognosis. More and more evidence show that the G protein coupled receptor P2RY14 plays a key role in the initiation and progression of various types of human cancer. The purpose of our study is to explore the correlation between P2RY14 and the prognosis of ovarian cancer patients and the relevant mechanism.Methods: First, the differentially expressed gene P2RY14 was screened from The Cancer Genome Atlas (TCGA) database. Explored possible P2RY14 related miRNAs and lncRNAs through multiple public databases, predicted and analyzed the expression level of candidate miRNAs and candidate lncRNAs that can bind to candidate miRNAs in OV through StarBase database. The TIMER database was used to comprehensively analyze the expression of tumor infiltrating immune cells, and to analyze the correlation between the expression level of P2RY14 and the level of immune cell infiltration in OV or the expression level of immune checkpoints.Results: Patients with P2RY14 overexpression had better overall survival (OS) and progression-free interval (PFI). In the Targetscan database, 22 upstream miRNAs that may bind to P2RY14 were predicted.According to the regulatory network constructed by the Cytoscape software, correlation analysis and the role of miRNAs in the prognosis of OV, we first determined that the candidate miRNAs were miR-34c-5p.Then, we predicted the upstream lncRNAs of miR-34c-5p in the StarBase database, the expression level of these lncRNAs in OV in the Gene Expression Profiling Interactive Analysis (GEPIA) database, and the role in prognosis. We determined that LINC00665 is the most potential lncRNA upstream of ovarian cancer miRNA (hsa-miR-34c-5p)-P2RY14. Then, we analyzed the results in the Timer database, suggesting that P2RY14 expression was positively correlated with CD8 + T Cell, CD4 + T Cell, Macrophage, Neutral and Dendritic cells, and negatively correlated with B cells. Meanwhile, P2RY14 was positively correlated with CD274 and PDCD1.Conclusions: P2RY14 can be used as a new predictive biomarker of ovarian cancer. Intervention of P2RY14 can affect the prognosis of ovarian cancer by affecting LINC00665-miR-34c-5p-P2RY14 axis.These findings provide a potential target for the development of anti-cancer strategies for ovarian cancer.

Transcriptome characteristics of epithelial cells from advanced non‐small cell lung cancer were revealed by single‐cell RNA sequencing

“…7 Evidence confirmed that P2RY14 may be involved in LUAD immune invasion and inhibit tumor cell immune 下Áescape within LUAD microenvironment. 8 A significant impact of JAML expression on LUAD prognosis and TME was observed, which highlights the importance of TME on LUAD patients. 9 Novel biomarkers related to TME in the OS of LUAD patients are urgently needed for predicting clinical management.…”

“…It performs an integral function in LUAD progression with significant heterogeneity 7 . Evidence confirmed that P2RY14 may be involved in LUAD immune invasion and inhibit tumor cell immune 下·escape within LUAD microenvironment 8 . A significant impact of JAML expression on LUAD prognosis and TME was observed, which highlights the importance of TME on LUAD patients 9 .…”

MicroRNA-34 Family in Cancers: Role, Mechanism, and Therapeutic Potential

“…Interestingly, lncRNA LINC00665 acts as a sponge for miR-34c-5p, preventing its interaction with P2RY14. This leads to the maintenance of low P2RY14 expression, which, in turn, correlates with poor prognosis and increased tumor immune infiltration in ovarian carcinoma [80]. The dysregulation of miR-34 family members and their downstream targets in ovarian cancer provides valuable insights into their significant roles in disease progression.…”