The purpose of this study is to validate GATE 8.1 Radiotherapy Monte Carlo (MC) simulation of a 6 MV Elekta Versa-HD linear accelerator at King Abdulaziz University Hospital (KAUH). The simulation data using GATE 8.1 was benchmarked against measured data at KAUH. The simulation comprises treatment head, Multi-Leaf Collimators (MLCs), and homogeneous water phantom, whereas the measured data was performed using MP3 phantom and PTW 31,010, 0.125 cm 3 Semiflex Chamber. Dose depth distribution and dose profiles were carried out at three different field sizes 10x10, 20x20, and 30 × 30 cm 2. The calculated TPR 20,10 for the simulated data was found to be 0.658, 1.1% less than the measured TPR 20,10 which was found to be 0.666. The obtained results indicate good agreement between the simulated and measured data, where Gamma Index 3%/3 mm criteria reached values of 97% and 90% for relative dose and dose profiles, respectively.