ND(1), a New Neutrophil Granulocyte Antigen

Verheugt, F.W.A.; Borne, A.E.G.Kr. v. d.; Noord-Bokhorst, J.G. v.; Nijenhuis, L.E.; Engelfriet, C.P.

doi:10.1159/000465192

Cited by 5 publications

(4 citation statements)

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“…should improve our understanding of the polymorphism, chemical composition, serologic reactivity, immunogenicity, and inheritance of this antigenic system. Recently, two new We feel that this granulocyte immunofluorescent procedure has easily been adapted Increased numbers of genetic studies concerning antigens limited to granulocytes antigenic determinants, called ND1 and NE1, also restricted to the surface of granulocytes, have been described [29,30] . Such investigations are beginning t o suggest that the granulocyte-specific antigen system is more polyallelic and polymorphic than formally believed.…”

Section: Discussionmentioning

confidence: 99%

A microimmunofluorescent assay to detect human granulocyte antigens and antibodies

Zaroulis

Jaramillo

1981

American J Hematol

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Granulocyte antibodies are important in the pathogenesis of neonatal granulocytopenia, in some varieties of idiopathic granulocytopenia, and in transfusion reactions, particularly since the development of leukocyte transfusion therapy for granulocytopenic patients. Previous assays for the detection of granulocyte-specific antigens and antibodies were cumbersome, complex, and unreliable. Thus we have now described a new microimmunofluorescent method to assay human granulocyte antigens and their antibodies. This sensitive and reliable technique allows for the preservation of human granulocyte antigenicity for at least 24 hours. The granulocyte-specific antigens of a Caucasian population, residing in metropolitan New York, were studied by this microimmunofluorescent method. We report the distribution of granulocyte-specific antigens in this population, together with the corresponding gene frequency. This microimmunofluorescent test should improve our understanding of antigenic systems restricted to single cell populations.

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Section: Discussionmentioning

confidence: 99%

A microimmunofluorescent assay to detect human granulocyte antigens and antibodies

Zaroulis

Jaramillo

1981

American J Hematol

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“…The cognate antigens are expressed on glycoproteins located on the neutrophil membrane. The first granulocyte antigen HNA-1a (formerly known as NA1) was discovered by Lalezari et al [2][3][4][5][6][7][8][9] during their investigation of a neonate with transient NAN and since then, many other antigens have been identified from sera of patients with autoantibodies or alloantibodies directed against these granulocyte antigens. The Granulocyte Immunobiology Working Party of the International Society of Blood Transfusion (ISBT) has now recognized nine polymorphic antigens (HNA-1a, -1b, -1c, -1d, -2, -3a, -3b, -4a, -4b, and 5a) assigned into five HNA systems and encoded by fourteen different alleles [10].…”

Section: Introductionmentioning

confidence: 99%

Phenotyping and Genotyping of HNA: Prevalence, Risk of Alloimmunization, and HNA Incompatibilities in Indians

Gogri

Parihar

Kulkarni

et al. 2022

Transfus Med Hemother

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Background: Antibodies to human neutrophil alloantigens (HNA) are involved in the pathophysiology of several clinical conditions including transfusion-related acute lung injury (TRALI), alloimmune and autoimmune neutropenia, and febrile nonhemolytic transfusion reactions leading to neutropenia. The cognate antigens are polymorphic structures expressed on several glycoproteins on the neutrophils, i.e., antigens HNA-1a, -1b, -1c, and -1d on Fc-γ-receptor IIIb; HNA-2 on CD177; HNA-3a and -3b on choline transporter-like protein 2; HNA-4a and -4b on CD11b/αM subunit of the αMβ2-integrin (CD11b/CD18, Mac-1, CR3); and HNA-5a and -5b on αL-subunit (CD11a) of the αLβ2 integrin (CD11a/CD18), leukocyte function associated molecule (LFA)-1. Currently, there is a lacuna of diagnostic methods for detection of HNA in India. This study aimed to determine the HNA frequencies in Indians, estimate the risk of alloimmunization, and prepare typed neutrophil panels, which can be used to detect HNA antibodies in neutropenia cases. Material and Methods: EDTA blood samples were collected from random 1,054 blood donors. HNA-2 was phenotyped on fresh EDTA samples using FITC labelled monoclonal anti-CD177 by flowcytometry. HNA-1 (FCGR3B) genotyping was carried out by DNA sequencing and PCR-RFLP. Antigens of HNA-3 (SLC44A2) and HNA-5 (ITGAL) were genotyped by PCR-RFLP using TaqαI and Bsp1286I restriction enzymes, respectively, while HNA-4 (ITGAM) was genotyped by PCR-SSP. Results: Allele frequencies of FCGR3B*01, FCGR3B*02, and FCGR3B*03 were found to be 0.433, 0.444, and 0.087, respectively. FCGR3B*01+*02+*03− was the most common genotype (33.78%). Ten individuals showed deficiency of FCGR3B individuals, while 23 showed hyperexpression, i.e., FCGR3B*01+*02+*03+. FCGR3B*04and *05 occurred with a frequency of 0.002 and 0.024. HNA-2 was found to be a high frequency antigen occurring in 98.8% population. Four percent individuals showed atypical expression of CD177 on their neutrophils. Allele frequencies of SLC44A2*01 and SLC44A2*02were 0.812 and 0.188, respectively, and that of ITGAM*01, ITGAM*02, ITGAL*01, and ITGAL*02 were 0.9546, 0.0454, 0.2372, and 0.7628, respectively. Conclusion: This is the first study in India to report the frequencies of HNA among blood donors. Typed neutrophil panels identified in the present study will enable us to investigate suspected cases of immune neutropenia in future.

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“…gen was tentatively called NE,, but the possibility that it is a product of an allele at the N D locus (Verheugt et al 1978) was not excluded. This is the more important since the gene frequency of NE, was complementary to the gene frequency of ND, (0.12 versus 0.88 in the Dutch population).…”

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confidence: 99%

“…Therefore, we tested this serum using the above techniques, with the granulocytes of two ND,-negative members of the family, described by Verheugt et al (1978) (i.e. donors 1 and 2).…”

mentioning

confidence: 99%

Neutrophil‐specific antigen NE₁ is not the antithetical allele of ND₁

Helmerhorst

Claas²,

Dalen

et al. 1981

Tissue Antigens

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ND(1), a New Neutrophil Granulocyte Antigen

Cited by 5 publications

References 1 publication

A microimmunofluorescent assay to detect human granulocyte antigens and antibodies

A microimmunofluorescent assay to detect human granulocyte antigens and antibodies

Phenotyping and Genotyping of HNA: Prevalence, Risk of Alloimmunization, and HNA Incompatibilities in Indians

Neutrophil‐specific antigen NE₁ is not the antithetical allele of ND₁

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ND(1), a New Neutrophil Granulocyte Antigen

Cited by 5 publications

References 1 publication

A microimmunofluorescent assay to detect human granulocyte antigens and antibodies

A microimmunofluorescent assay to detect human granulocyte antigens and antibodies

Phenotyping and Genotyping of HNA: Prevalence, Risk of Alloimmunization, and HNA Incompatibilities in Indians

Neutrophil‐specific antigen NE1 is not the antithetical allele of ND1

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Neutrophil‐specific antigen NE₁ is not the antithetical allele of ND₁