Objective: Cellular metabolism is a key regulator of CD4 + Foxp3 + regulatory T cell (Treg) homeostasis, but the foundational studies in this area use free fatty acid treatment as a proxy for plasma triglycerides. In vivo, plasma triglyceride is the main source of fatty acids for cells, not free fatty acids. Design/Results: Using apolipoprotein C-III transgenic and LDLr -/mice, we report that the loss of lipoprotein triglycerides transport in these models results in protection from DSS-colitis and accumulation of intestinal Tregs and plasma IL-10. Total loss of apoC-III increases colitis severity. Tregs exposed to apoC-III increase lipolysis and fatty acid oxidation and apoC-III inhibits Bodipytriglyceride uptake. Therapeutic treatment of WT mice with apoC-III-containing lipoproteins protects mice from colitis. Conclusion: Our data suggest that therapies that reduce apoC-III could have negative effects in patients who are at risk of IBD, and conversely, that apoC-III could be a new therapeutic target to stimulate intestinal Tregs and IL-10 for the management of IBD. These data identify apoC-III and lipoprotein metabolism as a novel regulator of tolerance in the intestine.
Summary Box
* What is already known about this subject:§ The relative capacity to use either glucose or FFA to generate acetyl CoA for mitochondrial fatty acid oxidation is a critical driver of Treg and T cell activity and proliferation. § ApoC-III is a known regulator of triglyceride and fatty acid metabolism in cells via LPL and LDLr endocytosis pathways § ApoC-III is reduced in Crohn's and Colitis patients.
* What are the new findings:§ We show that Tregs express triglyceride transporters, and that LDLr expression is enriched in Tregs from the mesenteric lymph nodes. § We show that T cells are capable of endocytosing triglyceride from lipoproteins, and this process is inhibited by apoC-III. § Tregs from apoC-III Tg are metabolically unique from WT Tregs and they upregulate the genes of lipolysis, and have an increase in basal respiration. § The inhibition of TAG endocytosis, using 2 different models (LDLr KO and apoC-IIItransgenic mice), protects mice from colitis and stimulates the accumulation of Tregs and IL-10 in the gut. § Intraperitoneal delivery of apoC-III on chylomicrons protects WT mice from DSS colitis.
* How might it impact on clinical practice in the foreseeable future?§ Due to the protective role apoC-III plays in these mouse models of colitis, IBD risk should be carefully considered before prescribing patient anti-apoC-III lipid-lowering therapies.the TAG for fuel through two processes: the hydrolysis of TAG to free fatty acids (FFA) via membrane-bound lipoprotein lipase (LPL) and the subsequent transport of those FFAs into the cell, or alternatively, endocytosis of lipoprotein TAG via the low-density lipoprotein receptor (LDLr), and the subsequent intracellular hydrolysis, oxidation, or storage of those FFAs. The way that a cell encounters a lipid fuel (either as a FFA bound to albumin or as a holoparticle lipoprotein) as sign...