NDM-5 and OXA-48 Co-producing Uropathogenic <i>Escherichia coli</i> Isolate: First Case in Korea

Jhang, Jinho; Wang, Hye Young; Yoo, Gilsung; Hwang, Gyu Yel; Uh, Young; Yoon, Kyung Joong

doi:10.3343/alm.2018.38.3.277

Cited by 10 publications

(8 citation statements)

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“…However, since 2015, multiple countries have inter-regional spread and bla OXA-48 is endemic in Malta and Turkey [ 147 ]. Further afield, extensively drug resistant (XDR) strains co-harbouring bla NDM and bla OXA-48 have been identified in the Middle East [ 177, 178 ], and bla OXA-48 strains have emerged in Canada [ 173 ], Algeria [ 179 ] and Korea [ 180 ]. Shewanella spp.…”

Section: Cpementioning

confidence: 99%

Extended-spectrum β-lactamase-producing and carbapenemase-producing Enterobacteriaceae

Wilson

Török

2018

Microbial Genomics

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Antimicrobial resistance (AMR) is a global public-health emergency, which threatens the advances made by modern medical care over the past century. The World Health Organization has recently published a global priority list of antibiotic-resistant bacteria, which includes extended-spectrum β-lactamase-producing Enterobacteriaceae and carbapenemase-producing Enterobacteriaceae. In this review, we highlight the mechanisms of resistance and the genomic epidemiology of these organisms, and the impact of AMR.

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Section: Cpementioning

confidence: 99%

Extended-spectrum β-lactamase-producing and carbapenemase-producing Enterobacteriaceae

Wilson

Török

2018

Microbial Genomics

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“…Following this discovery, two carbapenemase-producing Enterobacteriaceae (NDM-5 producing E. coli and NDM-1 producing Klebsiella pneumoniae ) showing distinct hydrolytic activity against imipenem were isolated from a traveler from Bangladesh in 2013 and Indonesia in 2014, respectively ( Nakano et al, 2014 ). Subsequently, in South Korea, NDM-9 and NDM-5 producing Klebsiella variicola and E. coli strains were recovered from a river in 2017 ( Di et al, 2017 ) and patients in 2018 ( Jhang et al, 2018 ), respectively, suggesting that environmental and clinical NDM-producing bacteria are in circulation.…”

Section: Introductionmentioning

confidence: 99%

Emergence of High Level Carbapenem and Extensively Drug Resistant Escherichia coli ST746 Producing NDM-5 in Influent of Wastewater Treatment Plant, Seoul, South Korea

et al. 2021

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High level carbapenem and extensively drug resistant (XDR) Escherichia coli strain N7, which produces a variant of New Delhi metallo-β-lactamase (NDM-5), was isolated from the influent of the Jungnang wastewater treatment plant located on Han River, Seoul, South Korea. Phenotypic and genotypic resistances to carbapenem were tested using agar and broth dilution methods, and polymerase chain reaction. Whole-genome sequencing was performed to characterize the genetic structure of strain N7. E. coli strain N7, which harbors the blaNDM–5 gene, showed high level of carbapenem resistance at concentrations of doripenem (512 mg/L) and meropenem (256 mg/L), and XDR to 15 antibiotics. Based on the genomic sequence analysis, two plasmids, a hybrid IncHI2/N-type and an IncX3 type, were present. The former contains a cluster (blaNDM–5-bleMBL-trpF-dsbD) bracketed by multi-insertional sequences, IS3000, ISAba125, IS5, and IS26. The latter carries the following resistance genes: blaCTX–14, aac(3)-IV, aadA1, aadA2, aph(3′)-Ia, aph(4)-Ia, sul1, sul2, sul3, dfrA12, fosA3, oqxA, oqxB, mph(A), and floR, and cmlA1. The chromosome, contig3, and contig5 also carry blaCTX–64 and mdf(A), tet(A), and erm(B), tet(M) and aadA22, respectively. Strain N7 also harbors virulence factors such as fimH, flu, ecpABCDE, sfmA, hlyE, and gadA. This study demonstrates the emergence of high level carbapenem resistant XDR E. coli strain N7 containing blaNDM–5 in aquatic environment, Seoul, South Korea. Due to the presence of mobile genetic elements, this strain could horizontally transfer resistance genes, including blaNDM–5 to environmental bacteria. Thus, it is necessary to conduct continuous surveillance for carbapenem resistance in various aquatic environments.

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“…Globally, the co-existence of bla NDM-5 and bla OXA-181 genes was first detected in K. pneumoniae, followed by Escherichia coli [2]. Similarly, in Korea, a bla NDM-5 and bla OXA-181 coproducing K. pneumoniae strain was first detected in 2014 [4], and by 2017, our hospital witnessed the first case caused by bla NDM-5 and bla OXA-48 co-producing uropathogenic E. coli [5].…”

mentioning

confidence: 99%

“…Blood samples drawn for determining the bacteremia were handled in the same way, previously [5]. Urine specimen was inoculated onto 5% sheep blood agar plate (KOMED Life Science Co., Seongnam, Korea) and MacConkey agar plate (BD Diagnostic Systems, Sparks, MD, USA), and then incubated overnight at 35°C.…”

mentioning

confidence: 99%

“…All stool swab specimens for CPE screening were inoculated in the selective chromogenic medium (CHROMagar KPC, Hangang, Gunpo, Korea). The CRE isolates obtained from the clinical specimen were tested by the Modified Hodge test, carbapenemase inhibition test, and CarbaNP test (bioMérieux, Durham, NC, USA) [6], and then, the CPE suspects were tested by XpertCarba-R assay (Cepheid, Sunnyvale, CA, USA) [5]. Each time a CPE was isolated, the subcultured colony was delivered to the Korea Centers for Disease and Prevention (KCDC) for the confirmation of the carbapenemase genotype, and KCDC gave feedback about the type of carbapenemase genes present.…”

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confidence: 99%

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Nosocomial Outbreak Caused by NDM-5 and OXA-181 Carbapenemase Co-producing Escherichia coli

et al. 2019

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Carbapenemase-producing Enterobacteriaceae (CPE) is an important and increasing threat to global health. From July to September 2017, 20 inpatients at a tertiary care hospital in Korea were either colonized or infected with carbapenem-resistant Escherichia coli strains. All of E. coli isolates co-produced bla NDM-5 and bla OXA-181 carbapenemase genes and shared ≥88% clonal relatedness on the basis of a cladistic calculation of the distribution of pulsed-field gel electrophoresis patterns. Rapid detection of CPE is one of the most important factors to prevent CPE dissemination because it takes long time for CPE to become negative.

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NDM-5 and OXA-48 Co-producing Uropathogenic Escherichia coli Isolate: First Case in Korea

Cited by 10 publications

References 9 publications

Extended-spectrum β-lactamase-producing and carbapenemase-producing Enterobacteriaceae

Extended-spectrum β-lactamase-producing and carbapenemase-producing Enterobacteriaceae

Emergence of High Level Carbapenem and Extensively Drug Resistant Escherichia coli ST746 Producing NDM-5 in Influent of Wastewater Treatment Plant, Seoul, South Korea

Nosocomial Outbreak Caused by NDM-5 and OXA-181 Carbapenemase Co-producing Escherichia coli

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