Near-chromosomal-level genome of the red palm weevil (Rhynchophorus ferrugineus), a potential resource for genome-based pest control

Eukaryotic genomes exhibit a dynamic interplay between single-copy sequences and repetitive DNA elements, with satellite DNA (satDNA) representing a substantial portion, mainly situated at telomeric and centromeric chromosomal regions. We utilized Illumina next-generation sequencing data from Adalia bipunctata to investigate its satellitome. Cytogenetic mapping via fluorescence in situ hybridization was performed for the most abundant satDNA families. In silico localization of satDNAs was carried out using the CHRISMAPP (Chromosome In Silico Mapping) pipeline on the high-fidelity chromosome-level assembly already available for this species, enabling a meticulous characterization and localization of multiple satDNA families. Additionally, we analyzed the conservation of the satellitome at an interspecific scale. Specifically, we employed the CHRISMAPP pipeline to map the satDNAs of A. bipunctata onto the genome of Adalia decempunctata, which has also been sequenced and assembled at the chromosome level. This analysis, along with the creation of a synteny map between the two species, suggests a rapid turnover of centromeric satDNA between these species and the potential occurrence of chromosomal rearrangements, despite the considerable conservation of their satellitomes. Specific satDNA families in the sex chromosomes of both species suggest a role in sex chromosome differentiation. Our interspecific comparative study can provide a significant advance in the understanding of the repeat genome organization and evolution in beetles.

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Molecular Insights into Red Palm Weevil Resistance Mechanisms of Coconut (Cocos nucifera) Leaves

Liu,

Yan,

Liu

et al. 2024

Plants

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Red palm weevil (RPW) (Rhynchophorus ferrugineus) threatens most palm species worldwide. This study investigated the molecular responses of coconut (Cocos nucifera) leaves to RPW infestation through metabolomics and transcriptomics analysis. An RPW insect attack model was developed by placing different RPW larval densitiesin coconut plants and measuring the relative chlorophyll content of different leaf positions and physiological indicators of dysfunction after RPW infestation. The metabolomic changes were detected in the leaves of 10, 20, 30, 40, and 50 days after infestation (DAI) using GC-MS. Certain metabolites (glycine, D-pinitol, lauric acid, allylmalonic acid, D-glucaro-1, 4-lactone, protocatechuic acid, alpha, and alpha-trehalose) were found to be possible indicators for distinct stages of infestation using metabolomics analysis. The influence on ABC transporters, glutathione, galactose, and glycolipid metabolism was emphasized by pathway analysis. Differentially expressed genes (DEGs) were identified at 5, 10, 15, and 20 DAI through transcriptomics analysis of infested coconut leaves, with altered expression levels under RPW infestation. The KEGG pathway and GO analysis revealed enrichment in pathways related to metabolism, stress response, and plant–pathogen interactions, shedding light on the intricate mechanisms underlying coconut–RPW interactions. The identified genes may serve as potential markers for tracking RPW infestation progression and could inform strategies for pest control and management.

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Near-chromosomal-level genome of the red palm weevil (Rhynchophorus ferrugineus), a potential resource for genome-based pest control

Cited by 4 publications

References 47 publications

Insights of the Rhynchophorus ferrugineus chemosensory system towards controlling its palm infestation problem: Progress from Omics research and technologies

Insights of the Rhynchophorus ferrugineus chemosensory system towards controlling its palm infestation problem: Progress from Omics research and technologies

Satellitome Analysis of Adalia bipunctata (Coleoptera): Revealing Centromeric Turnover and Potential Chromosome Rearrangements in a Comparative Interspecific Study

Molecular Insights into Red Palm Weevil Resistance Mechanisms of Coconut (Cocos nucifera) Leaves

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