Nano-technology is being frequently used in several aspect of life. Chemical and physical methods of nanoparticle synthesis generally involve high temperature, vacuum condition and harsh/toxic chemicals that cause adverse impact on environments including humans and are costly. Hence, there is a need of alternative method to synthesize nanoparticles. Biological approach that connects nanotechnology with plant extracts, fungi, honey etc. is the possible alternative of chemical and physical methods of nonparticles synthesis. Bio-tools are adapted in nano-biotechnology to promote the safer and sustainable application. This review focuses on the use of nano-catalysis and green chemistry for development of non-particles and their effective application in various biological and industrial, medical, agriculture etc.