2005
DOI: 10.1385/nbt:1:1:113
|View full text |Cite
|
Sign up to set email alerts
|

Near-Field Fluorescence Microscopy: An Optical Nanotool to Study Protein Organization at the Cell Membrane

Abstract: The ability to study the structure and function of cell membranes and membrane components is fundamental to understanding cellular processes. This requires the use of methods capable of resolving structures with nanometer-scale resolution in intact or living cells. Although fluorescence microscopy has proven to be an extremely versatile tool in cell biology, its diffraction-limited resolution prevents the investigation of membrane compartmentalization at the nanometer scale. Near-field scanning optical microsc… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
5

Citation Types

0
13
0

Year Published

2008
2008
2022
2022

Publication Types

Select...
5
4

Relationship

0
9

Authors

Journals

citations
Cited by 26 publications
(13 citation statements)
references
References 37 publications
0
13
0
Order By: Relevance
“…They use optical phenomena such as stimulated emission depletion [3,4], and interference fringes in the excitation beam [5]. Other approaches rely on the use of near-field phenomena like total internal reflection [6], near-field fluorescence microscopy [7,8], and surface plasmon coupled emission [9,10], or mechanically confined compartments such as sub-microfluidic channels [11] or nanoholes [12][13][14][15].…”
Section: Introductionmentioning
confidence: 99%
“…They use optical phenomena such as stimulated emission depletion [3,4], and interference fringes in the excitation beam [5]. Other approaches rely on the use of near-field phenomena like total internal reflection [6], near-field fluorescence microscopy [7,8], and surface plasmon coupled emission [9,10], or mechanically confined compartments such as sub-microfluidic channels [11] or nanoholes [12][13][14][15].…”
Section: Introductionmentioning
confidence: 99%
“…Recently, SNOM has been applied in material science and for biological samples measurement, where both the transmission and reflection SNOM configuration can be used . This method seems to be a very efficient for nanometric exploration of tissue sections, chromosomes, erythrocytes, biological membranes as well as cells . Of particular importance in life sciences is the possibility to conduct the SNOM investigations in liquid environments, that gives the opportunity to observe biological samples under physiological conditions.…”
Section: Introductionmentioning
confidence: 99%
“…In such a case, the NSOM microscope can be a very useful tool due to its high-resolution especially when combined with uorescent techniques. The latter one in conjunction with labeling methods could bring information about distribution of molecules of interest like, for example, surface receptors present in a cellular membrane [2,11]. Moreover, the measurements of optical properties in liquid environments will give the opportunity to observe changes of living cells in their natural environment [12].…”
Section: Introductionmentioning
confidence: 99%