Near infrared diffuse reflection and laser-induced fluorescence spectroscopy for myocardial tissue characterisation

Nilsson, Amk; Heinrich, D.; Olajos, Janos; Andersson‐Engels, Stefan

doi:10.1016/s1386-1425(97)00106-6

Cited by 43 publications

(37 citation statements)

References 29 publications

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“…The dominant differences of the spectra between lipids in plaque and in peri-adventitial tissue are shifts in the locations of the peaks, and these differences make it possible to differentiate between the lipids in plaques and in peri-adventitial tissue. The spectra of elastin, collagen water, and calcium obtained from refs [25][26][27]29]. All spectra are normalized to their respective maxima.…”

Section: Lipid Spectroscopymentioning

confidence: 99%

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Specific imaging of atherosclerotic plaque lipids with two-wavelength intravascular photoacoustics

Jansen

Steen

et al. 2015

Biomed. Opt. Express

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Abstract:The lipid content in plaques is an important marker for identifying atherosclerotic lesions and disease states. Intravascular photoacoustic (IVPA) imaging can be used to visualize lipids in the artery. In this study, we further investigated lipid detection in the 1.7-µm spectral range. By exploiting the relative difference between the IVPA signal strengths at 1718 and 1734 nm, we could successfully detect and differentiate between the plaque lipids and peri-adventitial fat in human coronary arteries ex vivo. Our study demonstrates that IVPA imaging can positively identify atherosclerotic plaques using only two wavelengths, which could enable rapid data acquisition in vivo. References and links1. E. Falk, P. K. Shah, and V. Fuster, "Coronary Plaque Disruption," Circulation 92(3), 657-671 (1995 Caplan, A. P. Burke, R. Virmani, J. Goldstein, and J. E. Muller, "Detection of lipid core coronary plaques in autopsy specimens with a novel catheter-based near-infrared spectroscopy system," JACC Cardiovasc. Imaging 1(5), 638-648 (2008 93-110 (1985).

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Section: Lipid Spectroscopymentioning

confidence: 99%

“…Normalized PA spectra for these non-lipid tissues are shown in Fig. 2(b) [26][27][28][29]. The relative PA difference ΔPA is defined as follows: PA λ are the PA amplitudes at the two wavelengths.…”

Section: Criteria For Lipid Detection and Differentiationmentioning

confidence: 99%

Specific imaging of atherosclerotic plaque lipids with two-wavelength intravascular photoacoustics

Jansen

Steen

et al. 2015

Biomed. Opt. Express

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“…The significance of the difference between averaged lifetimes of HCS and other tissues was evaluated using a paired t-test for the null hypothesis τ HCS -τ CT = 0 and τ HCS -τ MC = 0. The main fluorophores in the heart tissue were shown to be collagen and elastin [17,[19][20][21][22]; however, collagen is found in the heart tissues in several forms. The cardiac collagen of extracellular matrix consists of 85% of collagen type I [23].…”

Section: Resultsmentioning

confidence: 99%

“…More recently, the application of steady state fluorescence techniques to the ex vivo identification of different types of the heart tissues, including HCS, has been reported [18,19]. The main fluorophores in the heart were identified as collagen and elastin [17,[19][20][21][22], and it was inferred that the observed differences in fluorescence intensity are primarily determined by uneven amounts and the specific organisation of structural proteins rather than by the presence of specific fluorophores [19]. Since none of the types of the heart tissues possess distinct fluorescence spectra, the discrimination between them directly depends on the measurement conditions.…”

Section: Introductionmentioning

confidence: 99%

Time-resolved fluorescence spectroscopy of the heart tissues

Venius¹,

Bagdonas²,

Žurauskas³

et al. 2011

Lith. J. Phys.

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During the heart surgery there is a possibility to harm the conduction system of the heart (HCS), which may cause dangerous obstruction of the heart functionality. The muscular origin makes it complicated to distinguish HCS from the surrounding tissues; therefore, there is an immense necessity to visualise HCS during the operation time. Optical methods carry information about intrinsic properties of the tissue and provide the unique possibility to study the objects non-invasively. The experiments were performed on the human heart tissue specimens ex vivo. HCS, myocardium (MC), and connective tissue (CT) were preliminary marked by a pathologist and histologically approved after the spectral measurements. The spectrometer FLS920 (Edinburgh Instruments) was used for steady state and time-resolved fluorescence registration. Fluorescence was exited using a 405 nm pulsed laser. Spectral analysis revealed that at least three fluorophores are responsible for the emission in the region of 430-550 nm. According to the lifetimes, the fluorescing constituents in all tissues should be the same. The fractional components of fluorescence intensity revealed a similar composition of MC and HCS; however, quantitative differences were observed between HCS and CT.

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“…28 PCA is a powerful tool to get discriminate information that can differentiate various pathological situations in cell and tissue investigations. 29,30 The advantage of PCA over univariate methods is that it can look for the maximum variance in a large data set by comparing all the absorption wavelengths. In order to see the discrimination between control and amphetamine treated tissue in relation to protein bsheet formation, we used the amide II band for the chemometric evaluation through PCA.…”

mentioning

confidence: 99%

Amphetamine effects on brain protein structure and oxidative stress as revealed by FTIR microspectroscopy

et al. 2007

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Amphetamines are psychostimulants abused by man, that eventually leads to drug dependence. Amphetamine administration to rodents has been shown to provoke significant neurotoxicity involving dopaminergic nerve terminal degeneration. However, little information related to the effect of amphetamines on reactive oxygen species (ROS) production and neurotoxicity in brain is currently available. Herein we report the biochemical alterations of lipids and proteins in brain sections from amphetamine‐treated rodents using infrared microspectroscopy, immunohistochemistry, and immunoblotting. The spectroscopic changes reveal for the first time the formation of β‐sheet‐rich proteins in the cortex, but no significant protein alterations are visible in hippocampus region where hydroperoxide concentration is found to be lower relative to cortex. These result suggest that ROS generated by amphetamine‐mediated oxidative stress induce formation β‐sheet‐rich proteins which can be of amyloid β‐like character. © 2007 Wiley Periodicals, Inc. Biopolymers 86: 437–446, 2007.This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com

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Near infrared diffuse reflection and laser-induced fluorescence spectroscopy for myocardial tissue characterisation

Cited by 43 publications

References 29 publications

Specific imaging of atherosclerotic plaque lipids with two-wavelength intravascular photoacoustics

Specific imaging of atherosclerotic plaque lipids with two-wavelength intravascular photoacoustics

Time-resolved fluorescence spectroscopy of the heart tissues

Amphetamine effects on brain protein structure and oxidative stress as revealed by FTIR microspectroscopy

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