Near-Infrared Dye-Conjugated Amphiphilic Hyaluronic Acid Derivatives as a Dual Contrast Agent for In Vivo Optical and Photoacoustic Tumor Imaging

Miki, Koji; Inoue, Tomoyoshi; Kobayashi, Yasuhito; Nakano, Katsuya; Matsuoka, Hideki; Yamauchi, Fumio; Yano, Tetsuya; Ohe, Kouichi

doi:10.1021/bm501438e

Cited by 45 publications

(47 citation statements)

References 49 publications

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“…A recent report demonstrates that ICG that was directly conjugated to poly(ethylene glycol) grafted-HLA, where ICG served as the hydrophobic ligand, resulted in tumor signal, both fluorescence and photoacoustic, that was more than double that of femur. 54 However, the dose of ICG used in this study, 50 nmol per mouse, was 5 times greater than the dose used in this study.…”

Section: Resultsmentioning

confidence: 64%

Indocyanine Green-Loaded Nanoparticles for Image-Guided Tumor Surgery

et al. 2015

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Detecting positive tumor margins and local malignant masses during surgery is critical for long-term patient survival. The use of image-guided surgery for tumor removal, particularly with near-infrared fluorescent imaging, is a potential method to facilitate removing all neoplastic tissue at the surgical site. In this study we demonstrate a series of hyaluronic acid (HLA)-derived nanoparticles that entrap the near-infrared dye indocyanine green, termed NanoICG, for improved delivery of the dye to tumors. Self-assembly of the nanoparticles was driven by conjugation of one of three hydrophobic moieties: aminopropyl-1-pyrenebutanamide (PBA), aminopropyl-5β-cholanamide (5βCA), or octadecylamine (ODA). Nanoparticle self-assembly, dye loading, and optical properties were characterized. NanoICG exhibited quenched fluorescence that could be activated by disassembly in a mixed solvent. NanoICG was found to be nontoxic at physiologically relevant concentrations and exposure was not found to inhibit cell growth. Using an MDA-MB-231 tumor xenograft model in mice, strong fluorescence enhancement in tumors was observed with NanoICG using a fluorescence image-guided surgery system and a whole-animal imaging system. Tumor contrast with NanoICG was significantly higher than with ICG alone.

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Section: Resultsmentioning

confidence: 64%

Indocyanine Green-Loaded Nanoparticles for Image-Guided Tumor Surgery

et al. 2015

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“…By utilizing HA derivatives as contrast agents, tumors were clearly visualized by optical imaging as well as by OA tomography. 52 Because skin is composed of alternating lipophilic and hydrophilic layers, amphipathic compounds that possess both properties may be absorbed by the skin more effectively. Hydration is the most widely used and safest method to increase skin penetration for both hydrophilic and lipophilic permeants.…”

Section: Discussionmentioning

confidence: 99%

“…HA derivatives bearing both hydrophobic (indocyanine green) and hydrophilic (PEG) components were developed, but these substances did not possess the capability of enhancing penetration of light through skin. 52 To transport drugs effectively through the cellular membrane and deliver them into the intracellular environment, several smart carrier systems based on HA composites were developed containing both synthetic or natural polymers. 56 We speculate that the method of HA pretreatment described in this work may be also effective for drug delivery.…”

Section: Discussionmentioning

confidence: 99%

“…34,50,51 Amphiphilic HA derivatives bearing hydrophobic indocyanine green dye and hydrophilic PEG were synthesized through the use of condensation and copper-catalyzed click cyclization reactions. 52 The repair effect of HA and vaseline was demonstrated in dermal blood vessel study for peripheral vascular diseases. 53 Two kinds of fluorescent HA analogs, one serving as a normal imaging agent and the other used as a biosensitive contrast agent, were developed for the investigation of HA uptake and degradation.…”

Section: Introductionmentioning

confidence: 99%

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Optical clearing of skin enhanced with hyaluronic acid for increased contrast of optoacoustic imaging

Liopo

Tsyboulski

et al. 2016

J. Biomed. Opt

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Abstract. Enhanced delivery of optical clearing agents (OCA) through skin may improve sensitivity of optical and optoacoustic (OA) methods of imaging, sensing, and monitoring. This report describes a two-step method for enhancement of light penetration through skin. Here, we demonstrate that topical application of hyaluronic acid (HA) improves skin penetration of hydrophilic and lipophilic OCA and thus enhances their performance. We examined the OC effect of 100% polyethylene and polypropylene glycols (PPGs) and their mixture after pretreatment by HA, and demonstrated significant increase in efficiency of light penetration through skin. Increased light transmission resulted in a significant increase of OA image contrast in vitro. Topical pretreatment of skin for about 30 min with 0.5% HA in aqueous solution offers effective delivery of low molecular weight OCA such as a mixture of PPG-425 and polyethylene glycol (PEG)-400. The developed approach of pretreatment by HA prior to application of clearing agents (PEG and PPG) resulted in a ∼47-fold increase in transmission of red and near-infrared light and significantly enhanced contrast of OA images.

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“…Fifth, the use of VFR has not been validated in the infrapopliteal artery in the clinical setting, although we have recently reported the ability for dilating the vascular bed exists in the lower limb during increased oxygen demand supply in response to a given hyperemic stimulation and can be assessed in the same manner. 21 Finally, the pressure measurement distal to the lesion immediately after EVT was not attempted because residual dissections could be extended by advancing the pressure sensor into the artery undergoing EVT.…”

Section: Study Limitationsmentioning

confidence: 99%

Vascular Flow Reserve Immediately After Infrapopliteal Intervention as a Predictor of Wound Healing in Patients With Foot Tissue Loss

Fukunaga¹,

Fujii²,

Kawasaki³

et al. 2015

Circ: Cardiovascular Interventions

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Background-The purpose of this study was to verify whether the concept of coronary blood flow reserve can be applied to patients with critical limb ischemia who are undergoing endovascular treatment (EVT) for isolated infrapopliteal lesions. Methods and Results-Forty patients diagnosed with critical limb ischemia (Rutherford category 5) who were undergoing EVT for isolated infrapopliteal lesions were prospectively enrolled. All lesions were treated with conventional balloon angioplasty without stent placement. After successful EVT, a pressure/temperature sensor-tipped guidewire was positioned in the proximal popliteal artery. Using the thermodilution technique, the mean transit time (Tmn) was determined after bolus injections of 3-mL saline at baseline and at the onset of intra-arterial papaverine induced maximum hyperemia. Vascular flow reserve (VFR) was calculated as resting Tmn divided by hyperemic Tmn. Complete epithelialization of the reference wound (wound healing) was completely closed by either surgical or secondary intervention within 3 months after EVT. Wound healing was achieved in 22 patients after EVT (healing group) but was not achieved in 18 patients (nonhealing group). Postprocedural VFR was significantly lower in the nonhealing group than in the healing group (2.40; interquartile range, 2.00-3.08 versus 4.05; interquartile range, 3.60-4.60; P<0.0001). Receiver operating characteristic analysis revealed postprocedural VFR >3.6 as the best threshold value for complete wound healing after EVT. Univariate analysis revealed that postprocedural VFR >3.6 was a predictor of wound healing (P=0.0002). Conclusions-Advanced lower limb clinical setting may be caused by a poor capability of microvasculature. VFR, which is easily assessable, is useful in clinical risk stratification for patients with critical limb ischemia after EVT in the catheterization laboratory. Clinical Trial Registration

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Near-Infrared Dye-Conjugated Amphiphilic Hyaluronic Acid Derivatives as a Dual Contrast Agent for In Vivo Optical and Photoacoustic Tumor Imaging

Cited by 45 publications

References 49 publications

Indocyanine Green-Loaded Nanoparticles for Image-Guided Tumor Surgery

Indocyanine Green-Loaded Nanoparticles for Image-Guided Tumor Surgery

Optical clearing of skin enhanced with hyaluronic acid for increased contrast of optoacoustic imaging

Vascular Flow Reserve Immediately After Infrapopliteal Intervention as a Predictor of Wound Healing in Patients With Foot Tissue Loss

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