Near-Infrared Dyes: Probe Development and Applications in Optical Molecular Imaging

Nolting, Donald D.; Gore, John C.; Pham, Wellington

doi:10.2174/157017911796117223

Cited by 76 publications

(45 citation statements)

References 79 publications

(143 reference statements)

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“…Thioflavin is classified as a topologically activated dye that binds specifically to Aβ plaques (Nolting et al, 2011). This class of compounds possesses substantial conformational freedom while remaining in free solution and thus exhibits a relatively low fluorescence signal.…”

Section: Resultsmentioning

confidence: 99%

Identification of promethazine as an amyloid-binding molecule using a fluorescence high-throughput assay and MALDI imaging mass spectrometry

McClure

Chumbley

Reyzer

et al. 2013

NeuroImage: Clinical

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The identification of amyloid-binding compounds is a crucial step in the development of imaging probes and therapeutics for the detection and cure of Alzheimer's disease. Unfortunately, the process typically lags during the translation from in vitro to in vivo studies due to the impenetrable nature of the blood brain barrier (BBB). Here, we integrate fluorescence assay with MALDI imaging mass spectrometry to screen known compounds and repurpose their properties to enable the second function of binding to amyloid plaques. Through this approach, we identified an antihistamine compound, promethazine, that can bind to amyloid plaques. Finally, we demonstrate that promethazine is retained in the amyloid-burdened brain compared to a normal brain and that its distribution within the brain corroborates with that of amyloid plaques.

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Section: Resultsmentioning

confidence: 99%

Identification of promethazine as an amyloid-binding molecule using a fluorescence high-throughput assay and MALDI imaging mass spectrometry

McClure

Chumbley

Reyzer

et al. 2013

NeuroImage: Clinical

Self Cite

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“…The problem of poor anatomical imaging can be overcome by the use of MRI with the highest spatial resolution in combination with optical imaging [11]. In addition, limited depth penetration and high background signal can be partly overcome by the use of light in the near-infrared (NIR) range (700-900 nm), which is minimally absorbed by hemoglobin, muscle, and fat tissues, and minimizes autofluorescence of tissues [12]. Therefore, the NIR-fluorophore allows deeper tissue penetration of photons, which results in its potential application to noninvasive whole-body imaging for metastasis, especially peritoneal metastasis.…”

Section: Introductionmentioning

confidence: 99%

New whole-body multimodality imaging of gastric cancer peritoneal metastasis combining fluorescence imaging with ICG-labeled antibody and MRI in mice

Ito¹,

Ito²,

Matsushima³

et al. 2013

Gastric Cancer

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Background Peritoneal metastasis is the most frequent pattern of recurrence after curative surgery for gastric cancer. However, such a recurrence is difficult to detect by conventional computed tomography (CT) and magnetic resonance imaging (MRI) at an early stage. To improve the sensitivity and specificity of diagnostic imaging for peritoneal metastasis, we developed a new type of multimodality imaging combining fluorescence imaging with nearinfrared fluorophore (NIR)-labeled antibodies and MRI. Methods Dual optical imaging of peritoneal metastasis was carried out using luciferase-tagged gastric cancer cell lines and XenoLight CF750 or indocyanine green (ICG)-labeled anti-human epidermal growth factor receptor (EGFR) or CEA antibody as a probe in mice with Ivis in vivo imaging system.Results This whole-body fluorescent imaging system sensitively detected metastatic foci \1 mm in diameter in the peritoneal cavity noninvasively. Fluorescence imaging proved to be specific because the fluorescence signal was abolished by blocking with excess unlabeled antibody. Although this fluorescence imaging had higher sensitivity for detection of small-sized peritoneal metastases than MRI, it proved difficult to accurately determine organ distribution of the metastasis. We thus developed a multimodality imaging system by the fusion of the threedimensional fluorescence image with the MRI image and demonstrated its improved diagnostic accuracy over either method alone. Conclusion The present results suggest that multimodality imaging consisting of fluorescence imaging with NIRlabeled EGFR or CEA antibody and MRI allows sensitive, specific, and anatomically accurate detection of peritoneal metastasis noninvasively at an early stage.

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“…, the absolute optical property distributions) is needed to prevent the reconstruction from being biased. Although the reliable ROI a priori is normally obtainable in phantom experiments thanks to the extremely high target-to-background yield contrast and the preset background fluorescence properties, this condition might not be assured in realistic situations due to the incomplete clearance and non-specific binding effect of the administrated agent [46]. Therefore, further fundamental studies are necessary for developing a “soft-prior” regularization scheme for fluorescence-guided hemoglobin-DOT that is much more robust and unbiased in the presence of uncertainty in prior information [40].…”

Section: Discussionmentioning

confidence: 99%

Combined hemoglobin and fluorescence diffuse optical tomography for breast tumor diagnosis: a pilot study on time-domain methodology

Zhang

et al. 2013

Biomed. Opt. Express

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A combined time-domain fluorescence and hemoglobin diffuse optical tomography (DOT) system and the image reconstruction methods are proposed for enhancing the reliability of breast-dedicated optical measurement. The system equipped with two pulsed laser diodes at wavelengths of 780 nm and 830 nm that are specific to the peak excitation and emission of the FDA-approved ICG agent, and works with a 4-channel time-correlated single photon counting device to acquire the time-resolved distributions of the light re-emissions at 32 boundary sites of tissues in a tandem serial-to-parallel mode. The simultaneous reconstruction of the two optical (absorption and scattering) and two fluorescent (yield and lifetime) properties are achieved with the respective featured-data algorithms based on the generalized pulse spectrum technique. The performances of the methodology are experimentally assessed on breast-mimicking phantoms for hemoglobin- and fluorescence-DOT alone, as well as for fluorescence-guided hemoglobin-DOT. The results demonstrate the efficacy of improving the accuracy of hemoglobin-DOT based on a priori fluorescence localization.

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Near-Infrared Dyes: Probe Development and Applications in Optical Molecular Imaging

Cited by 76 publications

References 79 publications

Identification of promethazine as an amyloid-binding molecule using a fluorescence high-throughput assay and MALDI imaging mass spectrometry

Identification of promethazine as an amyloid-binding molecule using a fluorescence high-throughput assay and MALDI imaging mass spectrometry

New whole-body multimodality imaging of gastric cancer peritoneal metastasis combining fluorescence imaging with ICG-labeled antibody and MRI in mice

Combined hemoglobin and fluorescence diffuse optical tomography for breast tumor diagnosis: a pilot study on time-domain methodology

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