Near-infrared fluorescent probe for sensitive detection of Pb(II) ions in living cells

Bi, Jianheng; Fang, Mingxi; Wang, Jianbo; Xia, Shuai; Zhang, Yibin; Zhang, Jingtuo; Vegesna, Giri K.; Zhang, Shuwei; Tanasova, Marina; Luo, Fen‐Tair; Liu, Haiying

doi:10.1016/j.ica.2017.06.044

Cited by 35 publications

(24 citation statements)

References 27 publications

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“…21,38–40 In this paper, we chose near-infrared hemicyanine dyes with both near-infrared absorption and emission as an acceptor in a TBET strategy because of their near-infrared fluorescence emission, high molar absorptivity, and excellent photostability with Rhodamine-like fluorescence on–off switching mechanism by spirocyclization. 38,39,41 We designed three ratiometric fluorescent probes based on TBET and π -conjugation modulation strategies, and investigate the effect of different single and double sp 2 carbon–carbon bond connections between TPE and hemicyanine moieties on ratiometric fluorescence responses of the probes to pH changes in live cells (Scheme 2). In the TBET strategy, we prepared probe A by employing a palladium-catalyzed Suzuki reaction to electronically conjugate TPE donor ( 8 ) to hemicyanine acceptor ( 7a ).…”

Section: Resultsmentioning

confidence: 99%

Ratiometric Near-Infrared Fluorescent Probes Based On Through-Bond Energy Transfer and π-Conjugation Modulation between Tetraphenylethene and Hemicyanine Moieties for Sensitive Detection of pH Changes in Live Cells

Wang

Xia

et al. 2018

Bioconjugate Chem.

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In this paper, we present three ratiometric near-infrared fluorescent probes (A-C) for accurate, ratiometric detection of intracellular pH changes in live cells. Probe A consists of a tetraphenylethene (TPE) donor and near-infrared hemicyanine acceptor in a through-bond energy transfer (TBET) strategy, while probes B and C are composed of TPE and hemicyanine moieties through single and double sp carbon-carbon bond connections in a π-conjugation modulation strategy. The specific targeting of the probes to lysosomes in live cells was achieved by introducing morpholine residues to the hemicyanine moieties to form closed spirolactam ring structures. Probe A shows aggregation-induced emission (AIE) property at neutral or basic pH, while probes B and C lack AIE properties. At basic or neutral pH, the probes only show fluorescence of TPE moieties with closed spirolactam forms of hemicyanine moieties, and effectively avoid blind fluorescence imaging spots, an issue which typical intensity-based pH fluorescent probes encounter. Three probes show ratiometric fluorescence responses to pH changes from 7.0 to 3.0 with TPE fluorescence decreases and hemicyanine fluorescence increases, because acidic pH makes the spirolactam rings open to enhance π-conjugation of hemicyanine moieties. However, probe A shows much more sensitive ratiometric fluorescence responses to pH changes from 7.0 to 3.0 with remarkable ratio increase of TPE fluorescence to hemicyanine fluorescence up to 238-fold than probes B and C because of its high efficiency of energy transfer from TPE donor to the hemicyanine acceptor in the TBET strategy. The probe offers dual Stokes shifts with a large pseudo-Stokes shift of 361 nm and well-defined dual emissions, and allows for colocalization of the imaging readouts of visible and near-infrared fluorescence channels to achieve more precisely double-checked ratiometric fluorescence imaging. These platforms could be employed to develop a variety of novel ratiometric fluorescent probes for accurate detection of different analytes in applications of chemical and biological sensing, imaging, and diagnostics by introducing appropriate sensing ligands to hemicyanine moieties to form on-off spirolactam switches.

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Section: Resultsmentioning

confidence: 99%

Ratiometric Near-Infrared Fluorescent Probes Based On Through-Bond Energy Transfer and π-Conjugation Modulation between Tetraphenylethene and Hemicyanine Moieties for Sensitive Detection of pH Changes in Live Cells

Wang

Xia

et al. 2018

Bioconjugate Chem.

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“…Pb(II) ion detection in environment is crucial due to its hazardous characteristic which harmful to human and environment [1]- [3]. Short and long-term exposure of Pb(II) ion towards human may cause serious health illness such as fatal blood infection [4], behavioural abnormalities, kidney disorders, and neurological impairment [5]. The existing techniques for detection of Pb(II) ion are by costly, bulky and sophisticated instruments such as atomic absorption spectroscopy (AAS) [6], inductively couple plasma mass spectrometry (ICPMS) [7] and inductively couple plasma optical emission spectroscopy (ICP-OES) [8].…”

Section: Introductionmentioning

confidence: 99%

Localized SPR for Pb(II) ion sensing utilizing chitosan/reduced graphene oxide nanocomposite

Abdullah

Azeman

Kamaruddin

et al. 2021

JOE

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In this work, a simple and highly sensitive localized surface plasmon resonance (LSPR) technique had been developed for detection of Pb(II) utilizing chitosan/reduced graphene oxide (CS/rGO) nanocomposite as the sensing material. Gold nanoparticles (AuNP) had been incorporated with the nanocomposite material to induce strong LSPR responses. CS/rGO nanocomposite was coated on top of AuNP and had been utilized as active layer for Pb(II) ion sensing. The morphology and physical properties of gold nanoparticle-chitosan/reduced graphene oxide (AuNP-CS/rGO) nanocomposite was studied using field-emission scanning electron microscopy (FESEM), atomic force microscopy (AFM) and x-ray diffraction (XRD) analysis. FESEM exhibits a rough and wrinkle surface of AuNP-CS/rGO after the addition of rGO. AFM shows a higher surface roughness for AuNP-CS/rGO due to the presence of oxygen atoms in rGO where these oxygen atoms provide more sites for adsorption of Pb(II), hence enhance the sensitivity of the sensor. A good sensitivity of the LSPR sensor for detection of Pb(II) utilizing AuNP-CS/rGO was obtained which is 1.544 ppm-1 and the linearity of the sensor are 0.99 and 0.97. In stability study, AuNP-CS/rGO exhibits a good repeatability with relative standard deviation of 2% for 0.01 ppm.

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“…With the development of agriculture and industry, the application of heavy and transition metals becomes more and more prevalent, which leads to these kinds of metal ions entering ecological and environmental systems. These metal ions have posed significant damage to living organisms even at per million concentrations due to their toxicity, accumulation, and low rate of clearance, especially to the health of human beings [1,2,3,4]. Among these metal ions, Fe 3+ is the most abundant essential trace element in the organism.…”

Section: Introductionmentioning

confidence: 99%

A Novel Ruthenium(II) Polypyridyl Complex Bearing 1,8-Naphthyridine as a High Selectivity and Sensitivity Fluorescent Chemosensor for Cu2+ and Fe3+ Ions

Shibata

Ma³

et al. 2019

Molecules

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A novel ruthenium(II) polypyridyl complex bearing 1,8-naphthyridine was successfully designed and synthesized. This complex was fully characterized by EI-HRMS, NMR, and elemental analyses. The recognition properties of the complex for various metal ions were investigated. The results suggested that the complex displayed high selectivity and sensitivity for Cu2+ and Fe3+ ions with good anti-interference in the CH3CN/H2O (1:1, v/v) solution. The fluorescent chemosensor showed obvious fluorescence quenching when the Cu2+ and Fe3+ ions were added. The detection limits of Cu2+ and Fe3+ were 39.9 nmol/L and 6.68 nmol/L, respectively. This study suggested that this Ru(II) polypyridyl complex can be used as a high selectivity and sensitivity fluorescent chemosensor for Cu2+ and Fe3+ ions.

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Near-infrared fluorescent probe for sensitive detection of Pb(II) ions in living cells

Cited by 35 publications

References 27 publications

Ratiometric Near-Infrared Fluorescent Probes Based On Through-Bond Energy Transfer and π-Conjugation Modulation between Tetraphenylethene and Hemicyanine Moieties for Sensitive Detection of pH Changes in Live Cells

Ratiometric Near-Infrared Fluorescent Probes Based On Through-Bond Energy Transfer and π-Conjugation Modulation between Tetraphenylethene and Hemicyanine Moieties for Sensitive Detection of pH Changes in Live Cells

Localized SPR for Pb(II) ion sensing utilizing chitosan/reduced graphene oxide nanocomposite

A Novel Ruthenium(II) Polypyridyl Complex Bearing 1,8-Naphthyridine as a High Selectivity and Sensitivity Fluorescent Chemosensor for Cu2+ and Fe3+ Ions

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