Near-infrared fluorescent protein and bioluminescence-based probes for high-resolution <i>in vivo</i> optical imaging

Tiwari, Dhermendra K.; Tiwari, Manisha

doi:10.1039/d0ma00273a

Cited by 25 publications

(20 citation statements)

References 162 publications

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“…Furthermore, the AkaLumine-AkaLuc bioluminescence imaging system (Aka-BLI) at NIR wavelengths can detect resolution at the single-cell level in several live animals [11]. NIR imaging techniques using luciferin analogues are used in oncology, neuroscience and ethology [2,[12][13][14].…”

Section: Introductionmentioning

confidence: 99%

High Sensitivity In Vivo Imaging of Cancer Metastasis Using a Near-Infrared Luciferin Analogue seMpai

Nakayama

Saito

Hayashi

et al. 2020

IJMS

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Bioluminescence imaging (BLI) is useful to monitor cell movement and gene expression in live animals. However, D-luciferin has a short wavelength (560 nm) which is absorbed by tissues and the use of near-infrared (NIR) luciferin analogues enable high sensitivity in vivo BLI. The AkaLumine-AkaLuc BLI system (Aka-BLI) can detect resolution at the single-cell level; however, it has a clear hepatic background signal. Here, to enable the highly sensitive detection of bioluminescence from the surrounding liver tissues, we focused on seMpai (C15H16N3O2S) which has been synthesized as a luciferin analogue and has high luminescent abilities as same as AkaLumine. We demonstrated that seMpai BLI could detect micro-signals near the liver without any background signal. The solution of seMpai was neutral; therefore, seMpai imaging did not cause any adverse effect in mice. seMpai enabled a highly sensitive in vivo BLI as compared to previous techniques. Our findings suggest that the development of a novel mutated luciferase against seMpai may enable a highly sensitive BLI at the single-cell level without any background signal. Novel seMpai BLI system can be used for in vivo imaging in the fields of life sciences and medicine.

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Section: Introductionmentioning

confidence: 99%

High Sensitivity In Vivo Imaging of Cancer Metastasis Using a Near-Infrared Luciferin Analogue seMpai

Nakayama

Saito

Hayashi

et al. 2020

IJMS

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“…Since fluorescent proteins (FPs) and bioluminescent reporters are extremely useful to tag live biotherapeutic bacterial strains during in vivo preclinical assays (Cronin et al ., 2012 ; Gahan, 2012 ; Tiwari and Tiwari, 2020 ), in addition to GFP‐expressing EcN, we constructed EcN strains expressing a monomeric far‐red FP (mKATE2) (Shcherbo et al ., 2009 ; Chudakov et al ., 2010 ) and the bioluminescence luxCDABE operon from Photorhabdus luminescens (Winson et al ., 1998 ; Gahan, 2012 ). We followed the conjugation, integration and resolution strategy described previously with Ptac ‐gfp fusions.…”

Section: Resultsmentioning

confidence: 99%

Efficient markerless integration of genes in the chromosome of probiotic E. coli Nissle 1917 by bacterial conjugation

Seco

Fernández

2021

Microbial Biotechnology

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Summary The probiotic strain Escherichia coli Nissle 1917 (EcN) is a common bacterial chassis in synthetic biology developments for therapeutic applications given its long track record of safe administration in humans. Chromosomal integration of the genes of interest (GOIs) in the engineered bacterium offers significant advantages in genetic stability and to control gene dose, but common methodologies relying on the transformation of EcN are inefficient. In this work, we implement in EcN the use of bacterial conjugation in combination with markerless genome engineering to efficiently insert multiple GOIs at different loci of EcN chromosome, leaving no antibiotic resistance genes, vector sequences or scars in the modified bacterium. The resolution of cointegrants that leads to markerless insertion of the GOIs requires expression of I‐ Sce I endonuclease and its efficiency is enhanced by λ Red proteins. We show the potential of this strategy by integrating different genes encoding fluorescent and bioluminescent reporters (i.e. GFP, mKate2, luxCDABE ) both individually and sequentially. We also demonstrate its application for gene deletions in EcN (Δ flhDC ) and to replace the endogenous regulation of chromosomal locus (i.e. flhDC ) by heterologous regulatory elements (e.g. tetR ‐Ptet) in order to have an ectopic control of gene expression in EcN with an external inducer to alter bacterial behaviour (e.g. flagellar motility). Whole‐genome sequencing confirmed the introduction of the designed modifications without off‐target alterations in the genome. This straightforward approach accelerates the generation of multiple modifications in EcN chromosome for the generation of living bacterial therapeutics.

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“…Another sample‐based approach to deal with absorption and scattering is the usage of fluorophores that are redshifted. Due to less scattering and absorption of biological tissue in the longer wavelength range dyes and fluorophores that can be used in the near‐infrared and infrared range are increasingly adopted 84,85 …”

Section: Dealing With Absorption and Scatteringmentioning

confidence: 99%

“…Due to less scattering and absorption of biological tissue in the longer wavelength range dyes and fluorophores that can be used in the near-infrared and infrared range are increasingly adopted. 84,85…”

Section: Dealing With Absorption and Scatteringmentioning

confidence: 99%

Challenges and advances in optical 3D mesoscale imaging

Munck

Cawthorne

Escamilla-Ayala

et al. 2022

Journal of Microscopy

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Optical mesoscale imaging is a rapidly developing field that allows the visualisation of larger samples than is possible with standard light microscopy, and fills a gap between cell and organism resolution. It spans from advanced fluorescence imaging of micrometric cell clusters to centimetre-size complete organisms.However, with larger volume specimens, new problems arise. Imaging deeper into tissues at high resolution poses challenges ranging from optical distortions to shadowing from opaque structures. This manuscript discusses the latest developments in mesoscale imaging and highlights limitations, namely labelling, clearing, absorption, scattering, and also sample handling. We then focus on approaches that seek to turn mesoscale imaging into a more quantitative technique, analogous to quantitative tomography in medical imaging, highlighting a future role for digital and physical phantoms as well as artificial intelligence.

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Near-infrared fluorescent protein and bioluminescence-based probes for high-resolution in vivo optical imaging

Abstract:
This review presents the recent progress on NIR fluorescent protein and bioluminescence-based probes with high-resolution in vivo imaging techniques.

Cited by 25 publications

References 162 publications

High Sensitivity In Vivo Imaging of Cancer Metastasis Using a Near-Infrared Luciferin Analogue seMpai

High Sensitivity In Vivo Imaging of Cancer Metastasis Using a Near-Infrared Luciferin Analogue seMpai

Efficient markerless integration of genes in the chromosome of probiotic E. coli Nissle 1917 by bacterial conjugation

Challenges and advances in optical 3D mesoscale imaging

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Near-infrared fluorescent protein and bioluminescence-based probes for high-resolution in vivo optical imaging

Abstract: This review presents the recent progress on NIR fluorescent protein and bioluminescence-based probes with high-resolution in vivo imaging techniques.

Cited by 25 publications

References 162 publications

High Sensitivity In Vivo Imaging of Cancer Metastasis Using a Near-Infrared Luciferin Analogue seMpai

High Sensitivity In Vivo Imaging of Cancer Metastasis Using a Near-Infrared Luciferin Analogue seMpai

Efficient markerless integration of genes in the chromosome of probiotic E. coli Nissle 1917 by bacterial conjugation

Challenges and advances in optical 3D mesoscale imaging

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Abstract:
This review presents the recent progress on NIR fluorescent protein and bioluminescence-based probes with high-resolution in vivo imaging techniques.