Near-infrared in vivo fluorescence sensor with integrated dielectric emission filter

O’Sullivan, Thomas D.; Munro, Elizabeth A.; Conca, Christopher; Parashurama, Natesh; Zerda, Adam de la; Gambhir, Sanjiv S.; Harris, James S.; Levi, Ofer

doi:10.1364/cleo.2009.jwa49

Cited by 4 publications

(3 citation statements)

References 2 publications

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“…The final processing step is to deposit the dielectric emission filter that will block backscattered VCSEL light from reaching the top of the detector, yet allow fluorescence light to pass through. The emission filter is patterned on the detector mesa using a bilayer liftoff process [33] (Fig. 2f), and deposited with electron-beam evaporation.…”

Section: Semiconductor Processingmentioning

confidence: 99%

“…We utilize a lock-in technique to reduce the electrical noise in the system: the VCSEL is powered with a 1mA sinusoidal peak-to-peak current waveform at 23Hz overlaid on a 7mA current offset while the photodetector signal is recorded with a lockin amplifier using a 300ms time constant. [12,26,33]. Concentrations below 5nM cannot be reliably sensed since the detected photocurrent values vary due to changes in backscattered excitation light.…”

Section: In Vitro Sensitivitymentioning

confidence: 99%

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Implantable semiconductor biosensor for continuous in vivo sensing of far-red fluorescent molecules

O’Sullivan¹,

Munro²,

Parashurama³

et al. 2010

Opt. Express

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We have fabricated miniature implantable fluorescence sensors for continuous fluorescence sensing applications in living subjects. These monolithically integrated GaAs-based sensors incorporate a 675 nm vertical-cavity surface-emitting laser (VCSEL), a GaAs PIN photodiode, and a fluorescence emission filter. We demonstrate high detection sensitivity for Cy5.5 far-red dye (50 nanoMolar) in living tissue, limited by the intrinsic background autofluorescence. These low cost, sensitive and scalable sensors are promising for long-term continuous monitoring of molecular dynamics for biomedical studies in freely moving animals.

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Section: Semiconductor Processingmentioning

confidence: 99%

Section: In Vitro Sensitivitymentioning

confidence: 99%

Implantable semiconductor biosensor for continuous in vivo sensing of far-red fluorescent molecules

O’Sullivan¹,

Munro²,

Parashurama³

et al. 2010

Opt. Express

Self Cite

View full text Add to dashboard Cite

show abstract

“…A key component in fluorescence analysis is the optical filter that separates the excitation light from the fluorescence emission. Ideally, the optical filter component should be monolithically integrated to the lab-on-a-chip device, as well as simple to fabricate at low cost [ 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

Low Light CMOS Contact Imager with an Integrated Poly-Acrylic Emission Filter for Fluorescence Detection

Dattner

Yadid-Pecht

2010

Sensors

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This study presents the fabrication of a low cost poly-acrylic acid (PAA) based emission filter integrated with a low light CMOS contact imager for fluorescence detection. The process involves the use of PAA as an adhesive for the emission filter. The poly-acrylic solution was chosen due its optical transparent properties, adhesive properties, miscibility with polar protic solvents and most importantly its bio-compatibility with a biological environment. The emission filter, also known as an absorption filter, involves dissolving an absorbing specimen in a polar protic solvent and mixing it with the PAA to uniformly bond the absorbing specimen and harden the filter. The PAA is optically transparent in solid form and therefore does not contribute to the absorbance of light in the visible spectrum. Many combinations of absorbing specimen and polar protic solvents can be derived, yielding different filter characteristics in different parts of the spectrum. We report a specific combination as a first example of implementation of our technology. The filter reported has excitation in the green spectrum and emission in the red spectrum, utilizing the increased quantum efficiency of the photo sensitive sensor array. The thickness of the filter (20 μm) was chosen by calculating the desired SNR using Beer-Lambert’s law for liquids, Quantum Yield of the fluorophore and the Quantum Efficiency of the sensor array. The filters promising characteristics make it suitable for low light fluorescence detection. The filter was integrated with a fully functional low noise, low light CMOS contact imager and experimental results using fluorescence polystyrene micro-spheres are presented.

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Fabrication of an integrated 670nm VCSEL-based sensor for miniaturized fluorescence sensing

et al. 2010

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Near-infrared in vivo fluorescence sensor with integrated dielectric emission filter

Cited by 4 publications

References 2 publications

Implantable semiconductor biosensor for continuous in vivo sensing of far-red fluorescent molecules

Implantable semiconductor biosensor for continuous in vivo sensing of far-red fluorescent molecules

Low Light CMOS Contact Imager with an Integrated Poly-Acrylic Emission Filter for Fluorescence Detection

Fabrication of an integrated 670nm VCSEL-based sensor for miniaturized fluorescence sensing

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