Near-infrared light-activatable siRNA delivery by microcapsules for combined tumour therapy

Abstract

A polyelectrolyte microcapsule-based layer-by-layer (LbL) technique has been widely used as a multifunctional vehicle for combined tumor therapy. Meanwhile, with the rapid development of combined tumour therapy, the challenge for designing multifunctional drug delivery system has attracted much more attention. Herein, we developed a new type of microcapsule (MC) system called MPA@siRNA@DOX@MC, which conjugated with siRNA and DOX as well as ICG-Der-02 (MPA) by electrostatic absorption. MPA as indocyanine green (ICG) fluorescence dye, exhibiting high fluorescence emission and photothermal conversion ability under NIR laser irradiation, was uploaded onto this drug system for realizing the controllable drug release and cancer theranostics. In addition, the results revealed that MPA@siRNA@DOX@MC possessed several ideal properties including high drug-loading capacity, excellent siRNA transfection efficiency, siRNA sequence protection and remarkably improved tumour-targeting capacity. Moreover, the combined therapy based on this drug system displayed improved therapeutic efficacy and negligible side effects both in vivo and in vitro experiment. Ultimately, MPA@siRNA@DOX@MC drug delivery system successfully combined the photothermal therapy and chemotherapy with controlled siRNA sequence silencing may have a promising potential in combined tumor therapy.

Light-Activatable Transfection System Using Hybrid Vectors Composed of Thermosensitive Dendron Lipids and Gold Nanorods

“…Thus, heat generation under NIR light irradiation is useful to control the intracellular distribution of gene. NIR light-activatable other transfection systems such as indocyanine green-introduced layer-by-layer nanoparticles [35], poly(ethylene imine)-modified carbon nanotubes [36], and gold nanostar-incorporated liposomes [37] could also control or promote the transfection by enhanced endosomal escape of gene or liberation of gene from the complexes using light irradiation. Compared with these reports, our transfection system can control cellular internalization and endosomal escape process using dually light-and temperature-responsive moieties, AuNR and EDEG-DL, respectively ( Figures 5 and 6), which would provide more precisely controlled gene transfection using NIR light.…”

A novel magnetically guided, oxygen propelled CoPt/Au nanosheet motor in conjugation with a multilayer hollow microcapsule for effective drug delivery and light triggered drug release

“…42 In this regard, the natural polysaccharides, due to their biocompatibility and biodegradability, are introduced as the best candidates for biomedical applications. [43][44][45] Also, the multilayer microcapsules can load the drug and release it in a controlled manner under a specific external stimulus, such as temperature, 46 electricity, 47,48 ultrasound, 49,50 light or laser irradiation 51,52 using the magnetic fields 53,54 to achieve an improved treatment outcome. Among these, the laser irradiation, particularly in the near-infrared (NIR) light region (800-1200 nm), is more suited for the microcapsule opening, as it can be easily transmitted through the body, due to its lower absorption and scattering in tissues with fine spatial control over intensity.…”

Smart Layer-by-Layer Polymeric Microreactors: pH-Triggered Drug Release and Attenuation of Cellular Oxidative Stress as Prospective Combination Therapy

“…Recent progresses in the field of bioscience and polymer synthesis allow the fabrication of polymer capsules using alternative biodegradable synthetic and natural polymers, proteins, or inorganic nanoparticles, among others, their properties being tunable for specific biomedical requirements. ,,− Thus, taking advantage of this versatility and capability to fabricate polymer capsules with tailor-made properties, capsules have been fabricated for a wide variety of applications, including drug/protein/gene delivery vehicles, − polymer capsules for imaging applications, − or micro- and nanoreactors. ,− The latest contain active entities in their core and allow the diffusion of reagents and byproducts through the polymer shell. The active compounds [ e.g.…”