Near-infrared Raman spectroscopy to detect anti- Toxoplasma gondii antibodies in blood sera of domestic cats

Duarte, Janaína; Pacheco, Marcos Tadeu Tavares; Silveira, Landulfo; Machado, Rosangela Zacarias; Martins, Rodrigo Alvaro Brandão Lopes; Zângaro, Renato Amaro; Villaverde, Antônio Balbin

doi:10.1117/12.427767

Cited by 9 publications

(14 citation statements)

References 12 publications

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“…[149][150][151] Most of them are, in fact, related to the use of Raman nearinfrared spectroscopy for clinical analysis. 149,150 The pharmaceutical sector shows only one paper concerned with the use of NIR spectroscopy for identification of counterfeit drugs, a problem of social interest in Brazil.…”

Section: Nir Spectroscopy In Brazilmentioning

confidence: 99%

Near Infrared Spectroscopy: fundamentals, practical aspects and analytical applications

Pasquini¹

2003

J. Braz. Chem. Soc.

734

433

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Este trabalho visa prover uma revisão da teoria da Espectroscopia no Infravermelho Próximo (NIR) e das suas aplicações no campo das Ciências Analíticas. Ele é dirigido ao leitor que não possui um conhecimento profundo sobre espectroscopia vibracional mas que gostaria de ser introduzido às potencialidades analíticas desta técnica fascinante e, ao mesmo tempo, se tornar consciente das suas limitações. A teoria fundamental, uma revisão dos instrumentos modernos existentes e aplicações em inúmeros campos, são apresentados. Este trabalho não pretende suprir uma revisão bibliográfica intensiva mas se refere aos materiais recentes mais significativos e representativos encontrados na literatura técnica. Uma vez que este texto foi produzido em conseqüência do primeiro "workshop" em espectroscopia NIR, que ocorreu em Campinas -Brasil, como uma atividade preliminar ao 11º Encontro Nacional de Química Analítica (ENQA), ele também apresenta o estado da arte na Espectroscopia NIR no Brasil, mostrando seus resultados mais recentes e apontando a necessidade de se elevar esta tecnologia a um nível consistente com aquele de suas atividades econômicas. This paper intends to review the basic theory of Near Infrared (NIR) Spectroscopy and its applications in the field of Analytical Science. It is addressed to the reader who does not have a profound knowledge of vibrational spectroscopy but wants to be introduced to the analytical potentialities of this fascinating technique and, at same time, be conscious of its limitations. Essential theory background, an outline of modern instrument design, practical aspects, and applications in a number of different fields are presented. This work does not intend to supply an intensive bibliography but refers to the most recent, significant and representative material found in the technical literature. Because this paper has been produced as consequence of the First Workshop on Near Infrared Spectroscopy, whose venue was Campinas -Brazil, as a pre-conference activity of the XI National Meeting on Analytical Chemistry (ENQA), it also depicts the state of the art of NIR spectroscopy in Brazil, pointing out the current achievements and the need to take the technology to a level consistent with this country's economical activities.

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Section: Nir Spectroscopy In Brazilmentioning

confidence: 99%

Near Infrared Spectroscopy: fundamentals, practical aspects and analytical applications

Pasquini¹

2003

J. Braz. Chem. Soc.

734

433

View full text Add to dashboard Cite

show abstract

“…18,24 Briefly, the excitation source was a Ti:sapphire laser ͑Spectra Physics, model 3900S͒ pumped by a 6-W multiline argon laser ͑Spectra Physics, model 2709S͒, tuned to 830 nm. The Raman scattered signal from the sample was collected and coupled into the spectrometer entrance slit by a set of lenses.…”

Section: Raman Spectroscopymentioning

confidence: 99%

“…The ELISA assay is widely used for serology and was used as a gold standard for detecting IgG in the blood sera included in the PLS model. A preliminary study showing the potential of the dispersive near-IR ͑NIR͒ Raman spectroscopy technique for detecting T. gondii antibodies ͑IgG͒ in cat blood sera was previously reported by our group, 18 indicating feasibility for such optical approach.…”

Section: Introductionmentioning

confidence: 99%

“…This study indicated that Raman spectroscopy could be used to detect specific antibodies due to its high sensitivity for molecular detection. Application of Raman spectroscopy to detect the presence of antibodies against T. gondii in cat blood serum 18 and in human colostrum has been reported. 19 To be an effective and sensitive technique for biological material identification and classification, Raman spectroscopy requires an appropriate statistical method that can identify spectral differences that originate from differences in the sample composition that occur within the data set.…”

Section: Introductionmentioning

confidence: 99%

“…18,19 Raman spectroscopy is a useful tool for quantitative biological analysis as well as for clinical diagnosis in situ and ex situ, which can be used to reduce the risk of invasive procedures. [20][21][22] It provides a high specificity to identify and quantify metabolites and pharmaceutical products 23,24 and it has the potential to identify and characterize microorganisms ͑bacteria and fungi͒ rapidly.…”

Section: Introductionmentioning

confidence: 99%

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Near-infrared Raman spectroscopy to detect anti-Toxoplasma gondii antibody in blood sera of domestic cats: quantitative analysis based on partial least-squares multivariate statistics

et al. 2010

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Abstract. Toxoplasmosis is an important zoonosis in public health because domestic cats are the main agents responsible for the transmission of this disease in Brazil. We investigate a method for diagnosing toxoplasmosis based on Raman spectroscopy. Dispersive nearinfrared Raman spectra are used to quantify anti-Toxoplasma gondii ͑IgG͒ antibodies in blood sera from domestic cats. An 830-nm laser is used for sample excitation, and a dispersive spectrometer is used to detect the Raman scattering. A serological test is performed in all serum samples by the enzyme-linked immunosorbent assay ͑ELISA͒ for validation. Raman spectra are taken from 59 blood serum samples and a quantification model is implemented based on partial least squares ͑PLS͒ to quantify the sample's serology by Raman spectra compared to the results provided by the ELISA test. Based on the serological values provided by the Raman/PLS model, diagnostic parameters such as sensitivity, specificity, accuracy, positive prediction values, and negative prediction values are calculated to discriminate negative from positive samples, obtaining 100, 80, 90, 83.3, and 100%, respectively. Raman spectroscopy, associated with the PLS, is promising as a serological assay for toxoplasmosis, enabling fast and sensitive diagnosis.

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Blood Species Identification for Forensic Purposes Using Raman Spectroscopy Combined with Advanced Statistical Analysis

2009

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Forensic analysis has become one of the most growing areas of analytical chemistry in recent years. The ability to determine the species of origin of a body fluid sample is a very important and crucial part of a forensic investigation. We introduce here a new technique which utilizes a modern analytical method based on the combination of Raman spectroscopy and advanced statistics to analyze the composition of blood traces from different species. Near-infrared Raman spectroscopy (NIR) was used to analyze multiple dry samples of human, canine, and feline blood for the ultimate application to forensic species identification. All of the spectra were combined into a single data matrix, and the number of principle components that described the system was determined using multiple statistical methods such as significant factor analysis (SFA), principle component analysis (PCA), and several cross-validation methods. Of the six principle components that were determined to be present, the first three, which contributed over 90% to the spectral data of the system, were used to form a three-dimensional scores plot that clearly showed significant separation between the three groups of species. Ellipsoids representing a 99% confidence interval surrounding each species group showed no overlap. This technique using Raman spectroscopy is nondestructive and quick and can potentially be performed at the scene of a crime.

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Near-infrared Raman spectroscopy to detect anti- Toxoplasma gondii antibodies in blood sera of domestic cats

Cited by 9 publications

References 12 publications

Near Infrared Spectroscopy: fundamentals, practical aspects and analytical applications

Near Infrared Spectroscopy: fundamentals, practical aspects and analytical applications

Near-infrared Raman spectroscopy to detect anti-Toxoplasma gondii antibody in blood sera of domestic cats: quantitative analysis based on partial least-squares multivariate statistics

Blood Species Identification for Forensic Purposes Using Raman Spectroscopy Combined with Advanced Statistical Analysis

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