Near infrared spectroscopic assessment of loosely and tightly bound cortical bone water

Ailavajhala, Ramyasri; Querido, William; Rajapakse, Chamith S.; Pleshko, Nancy

doi:10.1039/c9an02491c

Cited by 13 publications

(24 citation statements)

References 38 publications

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“…Analysis of specific second derivative NIR peak intensities and ratios allows quantification of several parameters directly associated with water and protein content 40 . Spectral absorbance peaks of water are observed at 5300, 7100 and 8600 cm -1 , with each peak related to water molecules present in different tissue environments, such as free and hydrogen-bound tissue water 20,22,[40][41][42][43][44][45][46] . Their relative intensities reflect differences in tissue type and composition 22 , and thus, the empirical evaluation of the water peak ratios may inform changes in molecular environments within the TEC, and correlations to TEC construct quality and related in vivo repair tissue quality.…”

Section: Discussionmentioning

confidence: 99%

Near infrared spectroscopic assessment of engineered cartilage for implantation in a pre-clinical model

Falcon

Kandel

Querido

et al. 2022

Journal of Cartilage & Joint Preservation

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This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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Section: Discussionmentioning

confidence: 99%

Near infrared spectroscopic assessment of engineered cartilage for implantation in a pre-clinical model

Falcon

Kandel

Querido

et al. 2022

Journal of Cartilage & Joint Preservation

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“…Research has largely been focused on the material level property changes directly associated with the altered collagen followed by a focus on the poor mineral quality ( Camacho et al, 2003 ; Surowiec et al, 2020 ). Water, particularly loosely and tightly bound collagen-related water, is hypothesized to play a role in governing bone health in OI through supporting formation and maintenance of collagen structure and possibly mineralization ( Ailavajhala et al, 2020 ; Kramer et al, 2000 ). Despite this link, little is known about how bone water is altered under OI conditions.…”

Section: Bone Water With Age and Diseasementioning

confidence: 99%

Bone hydration: How we can evaluate it, what can it tell us, and is it an effective therapeutic target?

2022

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Water constitutes roughly a quarter of the cortical bone by volume yet can greatly influence mechanical properties and tissue quality. There is a growing appreciation for how water can dynamically change due to age, disease, and treatment. A key emerging area related to bone mechanical and tissue properties lies in differentiating the role of water in its four different compartments, including free/pore water, water loosely bound at the collagen/mineral interfaces, water tightly bound within collagen triple helices, and structural water within the mineral. This review summarizes our current knowledge of bone water across the four functional compartments and discusses how alterations in each compartment relate to mechanical changes. It provides an overview on the advent of- and improvements to- imaging and spectroscopic techniques able to probe nano-and molecular scales of bone water. These technical advances have led to an emerging understanding of how bone water changes in various conditions, of which aging, chronic kidney disease, diabetes, osteoporosis, and osteogenesis imperfecta are reviewed. Finally, it summarizes work focused on therapeutically targeting water to improve mechanical properties.

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“…In bone, water molecules can occupy different compartments, such as within pores (free water), loosely bound to protein and mineral surfaces or tightly bound between collagen chains and within the mineral crystal lattice. Recently, Ailavajhala et al [ 47 ] showed that NIR spectroscopy can be applied to differently assess both loosely and tightly bound water in bone. They used a variety of sample preparation procedures (dehydration, deuteration, demineralization, denaturing) to regulate composition and molecular environments of cortical human bone samples.…”

Section: Application Of Vibrational Spectroscopy For Connective Timentioning

confidence: 99%

“…Typical absorbance bands of relevance to the analysis of NIR spectra of connective tissues [41][42][43][44][45][46][47][48][49]…”

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Applications of Vibrational Spectroscopy for Analysis of Connective Tissues

2021

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Advances in vibrational spectroscopy have propelled new insights into the molecular composition and structure of biological tissues. In this review, we discuss common modalities and techniques of vibrational spectroscopy, and present key examples to illustrate how they have been applied to enrich the assessment of connective tissues. In particular, we focus on applications of Fourier transform infrared (FTIR), near infrared (NIR) and Raman spectroscopy to assess cartilage and bone properties. We present strengths and limitations of each approach and discuss how the combination of spectrometers with microscopes (hyperspectral imaging) and fiber optic probes have greatly advanced their biomedical applications. We show how these modalities may be used to evaluate virtually any type of sample (ex vivo, in situ or in vivo) and how “spectral fingerprints” can be interpreted to quantify outcomes related to tissue composition and quality. We highlight the unparalleled advantage of vibrational spectroscopy as a label-free and often nondestructive approach to assess properties of the extracellular matrix (ECM) associated with normal, developing, aging, pathological and treated tissues. We believe this review will assist readers not only in better understanding applications of FTIR, NIR and Raman spectroscopy, but also in implementing these approaches for their own research projects.

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Near infrared spectroscopic assessment of loosely and tightly bound cortical bone water

Abstract: NIR spectroscopy can differentiate water loosely bound to bone tissue, and tightly bound to either collagen or mineral.

Cited by 13 publications

References 38 publications

Near infrared spectroscopic assessment of engineered cartilage for implantation in a pre-clinical model

Near infrared spectroscopic assessment of engineered cartilage for implantation in a pre-clinical model

Bone hydration: How we can evaluate it, what can it tell us, and is it an effective therapeutic target?

Applications of Vibrational Spectroscopy for Analysis of Connective Tissues

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