Near‐IR Metal Enhanced Fluorescence and Controlled Colloidal Aggregation

Anderson, Jocelyn; Griffiths, Mark Z.; Williams, John G.; Grone, Daniel L.; Steffens, Dave L.; Middendorf, Lyle M.

doi:10.1002/9780470642795.ch5

Cited by 2 publications

(3 citation statements)

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“…Fluorescence emission scans of SAv-800CW in 5 mM NaCl with and without AgNP (Figure ) demonstrate the ability to achieve 300-fold signal enhancement in solution. This is consistent with previous work and demonstrates a larger enhancement than immobilizing the dye on surfaces. , We attribute this difference to the ability to locate the fluorophores within and between the aggregating AgNP in solution as opposed to locating on one side of the aggregates on a surface. Further investigation is required to determine the exact mechanism that provides the extra enhancement in solution and is beyond the scope of this study.…”

Section: Resultssupporting

confidence: 91%

“…Third, aggregation has been known to broaden and red-shift the resonant frequency of AgNPs by enabling multipole plasmon modes and thus increases the interaction with NIR light [see Figure b]. Aggregation of AgNP has also been shown to generate small, localized, enhanced electromagnetic fields, known as “hot spots”, thereby enabling significant SEF of appropriately located fluorophores. ,,, Therefore, a combination of enhanced near-field due to aggregation with low far-field losses and the maximized quality factor makes NIR-SEF with AgNPs in solution a powerful technology.…”

Section: Resultsmentioning

confidence: 99%

“…When certain criteria are met, the radiative decay dominates nonradiative processes, which along with the enhanced excitation rate can significantly increase the quantum yield. Enhanced signal intensity from SEF along with lower background via NIR fluorophores has been shown to provide substantial improvement to fluorescence sensitivity. , NIR-SEF has been demonstrated previously with gold nanostructures on surfaces, − gold nanoparticles in solution, − silver nanostructures on surfaces, ,,, and silver nanoparticles in solution. , …”

Section: Introductionmentioning

confidence: 90%

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A Near-Infrared, Surface-Enhanced, Fluorophore-Linked Immunosorbent Assay

et al. 2013

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The enzyme-linked immunosorbent assay is commonly used for research and clinical applications but typically suffers from a limited linear range and is difficult to multiplex. The fluorophore-linked immunosorbent assay is a closely related technique with good linear range and the ability to detect multiple antigens simultaneously but is typically less sensitive. Here, we demonstrate a near-infrared, surface-enhanced fluorophore-linked immunosorbent assay with sensitivity comparable to its enzyme-linked counterpart. A 59-fold enhancement to sensitivity (slope of linear fit) and an 8-fold improvement in LOD are demonstrated on a direct assay with rabbit immunoglobulin-G as a model system. The technique is also tested on a clinically relevant assay to detect alpha-fetoprotein, in which a 42-fold enhancement to sensitivity is demonstrated along with a 16-fold improvement in LOD. The technique enables these accomplishments while maintaining the entire traditional assay protocol and simply adding two steps at the end. This technique may prove superior to current protocols for biomarker research and clinical diagnoses, which require high sensitivity along with quantitation over an extended range.

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Section: Resultssupporting

confidence: 91%