This research presents the influence of manufacturing processes and heat treatments on the resulting microstructures and mechanical properties of an API 5L PSL2 seamless steel pipe. Three different conditions are considered -as rolled, normalized and quenched and tempered -to obtain the grades X42R, X42N and X70Q, respectively. Scanning electron microscopy techniques was used to characterize the resultant microstructures. Tensile, hardness, impact, fracture toughness (J integral) and fatigue crack growth tests (da/dN x ΔK) were used to study the materials mechanical behavior. The results show the possibility of achieving API grades for a seamless pipeline steel, through suitable heat treatments. The microstructural modifications and mechanical properties changes observed showed a remarkable structure-properties relationship of the steel, and attempt to a proper selection as required by the structural design. The quenching and tempering process increased tensile mechanical properties and fracture toughness, but combined to a significant decrease in fatigue crack growth resistance.