NEAR ULTRAVIOLET INDUCTION OF GROWTH DELAY STUDIED IN A MENAQUINONE‐DEFICIENT MUTANT OF <i>BACILLUS SUBTILIS</i>

Taber, Harry; Pomerantz, B J; Halfenger, G. M.

doi:10.1111/j.1751-1097.1978.tb07694.x

Cited by 19 publications

(5 citation statements)

References 25 publications

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“…Accordingly, rates of menaquinone synthesis from [l4Clshikimic acid (Taber et al, 1978) were measured for cultures of strain RB 163(men) growing with various shikimic acid concentrations in the presence and absence of either kanarnycin or gentamkin. The results for kanamycin are shown in Fig.…”

Section: Effect Of Kanamycin On Menaquinone Biosynthesismentioning

confidence: 99%

Involvement of Menaquinone in the Active Accumulation of Aminoglycosides by Bacillus subtilis

Taber¹,

Sugarman²,

Halfenger³

1981

Microbiology

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Accumulation of aminoglycoside antibiotics by bacteria requires energy, and it appears that this must be derived from electron transport occurring within the cytoplasmic membrane. Dependence of aminoglycoside accumulation on cellular menaquinone content was examined using a menaquinone auxotroph of bacillus subtilis. This dependence manifested itself only when the menaquinone concentration was decreased to less than 10% of normal. The restricted aminoglycoside accumulation observed under these conditions was closely correlated with susceptibility to growth inhibition by the antibiotics. Evidence of saturation of the accumulation system was observed at low menaquinone concentrations, an effect not seen when menaquinone deficiency was relieved by supplying adequate shikimic acid (a menaquinone precursor) to the auxotroph. Lipophilic quinones may play two roles in aminoglycoside accumulation by bacteria: (i) as a binding site or part of a carrier complex: and (ii) as a crucial component of the electron transport system in maintaining the proton electrochemical gradient.

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Section: Effect Of Kanamycin On Menaquinone Biosynthesismentioning

confidence: 99%

Involvement of Menaquinone in the Active Accumulation of Aminoglycosides by Bacillus subtilis

Taber¹,

Sugarman²,

Halfenger³

1981

Microbiology

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“…Finally, recent work of Madden et al (1977) shows that MQ-8 is extremely sensitive to near UV in E. coli. However, evidence of a more resistant form lies in the recent finding (Taber et al, 1978) that menaquinone appears to be a target molecule for near-UV-induced growth delay in Bacillus subtilis, a system requiring much higher fluences (several hundred kJ/m2), and probably utilizing a different mechanism, than in E. coli. wbiquinone-8 inactivation in E. coli not only has the wrbng action spectrum (Werbin et al, 1974), but also shows far too high an F,, (about 420 kJ/mz at 334 nm).]…”

Section: Action Spectrummentioning

confidence: 99%

ULTRAVIOLET ACTION SPECTRUM (238–405 nm) FOR INHIBITION OF GLYCINE UPTAKE IN E. COLI*

Ascenzi

Jagger

1979

Photochem & Photobiology

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Abstract— Initial rate of uptake of 3H‐glycine by Escherichia coli B/r was measured immediately after irradiation with monochromatic light. Uptake was proportional to time for at least 2 min in both control and irradiated samples. Inhibition of uptake is an exponential function of fluence to about 20% remaining activity, beyond which it is much more resistant to irradiation, suggesting two different uptake systems. The principal (sensitive) system shows an F37 of 2.2 kJ/m2 at 280 nm and 110 kJ/m2 at 334 nm. The response is independent of cell killing and of presence of the rel gene. The chromophore remains unidentified, although an action spectrum suggests a protein chromophore in the far‐UV (below 300 nm) region and a menaquinone chromophore in the near‐ultraviolet (above 300 nm). A 10–20%, stimulation of uptake rate, which we cannot account for, is observed at low fluences (generally below 100 kJ/m2) at 313, 366 and 405 nm, but not at 334 nm.

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“…Recent studies concerning the effects of UVA on P. aeruginosa demonstrate a correlation between catalase levels and UVA sensitivity [39]. On the other hand, sub-lethal UVA exposures induce a reduction in growth rate without loss of viability in several bacterial species [40][41][42][43], including P. aeruginosa [39]. The growth delay effect induced by UVA under the conditions used during pre-irradiations in this study is shown in Figure 5.…”

Section: Effect Of Previous Exposure To Low Uva Fluencies On Hp Efficmentioning

confidence: 90%

Factors Capable of Modifying the Response of Pseudomonas aeruginosa to the Inactivation Induced by Heterogeneous Photocatalysis

Araujo

Oppezzo

Ibáñez

et al. 2013

International Journal of Chemical Reactor Engineering

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The heterogeneous photocatalysis (HP) procedure has been demonstrated to be an interesting method for disinfecting water. It is effective for inactivating Pseudomonas aeruginosa, but its effectiveness may be reduced due to the action of several factors which are able to affect bacterial radio-sensitivity. The results reported here show the influence of nutritional stress and pre-exposure to sub-lethal UVA doses on the efficiency of the inactivation of P. aeruginosa by HP. Both previous exposures to low UVA fluencies and nutrient deprivation induce bacterial resistance to this process, in concordance with previous observations about the lethal effect of direct UVA irradiation. Starvation plus pre-irradiation did not have synergistic or antagonist effects. Kinetic parameters are presented for all three cases. These factors should be taken into account in the design of a water treatment process.

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NEAR ULTRAVIOLET INDUCTION OF GROWTH DELAY STUDIED IN A MENAQUINONE‐DEFICIENT MUTANT OF BACILLUS SUBTILIS

Cited by 19 publications

References 25 publications

Involvement of Menaquinone in the Active Accumulation of Aminoglycosides by Bacillus subtilis

Involvement of Menaquinone in the Active Accumulation of Aminoglycosides by Bacillus subtilis

ULTRAVIOLET ACTION SPECTRUM (238–405 nm) FOR INHIBITION OF GLYCINE UPTAKE IN E. COLI*

Factors Capable of Modifying the Response of Pseudomonas aeruginosa to the Inactivation Induced by Heterogeneous Photocatalysis

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